We narrowed to 4,284 results for: TIL

1. pLenti-PGK-flag-RAP1B-N17-neo

   Plasmid

   #156176

   Purpose

   Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BE3(E63Q)-P2A-EGFP (pRZ189)

   Plasmid

   #123613

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(E63Q)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E63Q in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-PGK-RAP2A-N17-neo

   Plasmid

   #156179

   Purpose

   Lentiviral vector for expression of RAP2A-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

   Plasmid

   #105803

   Purpose

   Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-FLAG; ORF2: TEV-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. nCas9-UGI control for Target-AID (pRZ1047)

   Plasmid

   #131299

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

   Plasmid

   #113162

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

   Plasmid

   #136659

   Purpose

   Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:TH1477 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCMVblast_SLC38A2_N82A

   Plasmid

   #156181

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   CMV

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pInducer20_SLC38A2_N82A

   Plasmid

   #156183

   Purpose

   Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   TRE

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IL2-AHDC2-Cherry in pmCherryN1

    Plasmid

    #187291

    Purpose

    Express pmCherry-IL2-AH-deltaC2

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    AH-deltaC2

    Promoter

    CMV

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLL5.0_shRNAHsCavin1_MmCavin1_EGFP

    Plasmid

    #229689

    Purpose

    Lentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)

    Depositor

    Alpha Yap

    Article

    Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

    Inserts

    shRNA for hCavin1 (CAVIN1 Human)
    mCavin1 (Cavin1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    LTR viral promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IL2-mCherry-AHA740D, E758K in pmCherryN1

    Plasmid

    #187285

    Purpose

    Express IL2-AH A740D, E758K-mCherry

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    AH A740D, E758K

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

    Plasmid

    #187647

    Purpose

    Encodes gRNA to correct the SFTPC I73T mutation

    Depositor

    Darrell Kotton

    Article

    Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

    Insert

    I73T gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDR111_mannose_Klf4Gata-3

    Plasmid

    #188401

    Purpose

    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Inserts

    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SITS-Cavin4b-EGFP

    Plasmid

    #176019

    Purpose

    Leishmania cell free expression of zebrafish Cavin4b-EGFP. Parton lab clone GRJ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SITS-Cavin4b-mCherry

    Plasmid

    #176023

    Purpose

    Leishmania cell free expression of zebrafish Cavin4b-mCherry. Parton lab clone GWR

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cavin4b-mCherry

    Plasmid

    #176008

    Purpose

    Mammalian expression of zebrafish Cavin4b-mCherry. Parton lab clone HYO

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cavin4b(PRDmt)-mCherry

    Plasmid

    #176009

    Purpose

    Mammalian expression of zebrafish Cavin4b(PRDmt)-mCherry. Parton lab clone JMD

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cavin4b-EGFP

    Plasmid

    #176011

    Purpose

    Mammalian expression of zebrafish Cavin4b-EGFP. Parton lab clone HXM

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pME-Cavin4b_PRDmt

    Plasmid

    #176017

    Purpose

    Multisite gateway middle entry clone containing zebrafish Cavin4b_PRDmt with no stop codon. Parton lab clone JLY.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (PRDmt) (cavin4b Zebrafish)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cavin4b in pBluescript(SK+)

    Plasmid

    #126566

    Purpose

    For generating RNA probes for in situ hybridisation. Parton lab clone IRZ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3E-BIN1_tv8

    Plasmid

    #126574

    Purpose

    Multisite gateway middle entry vector containing human BIN1 transcriptional variant 8 (muscle specific) with no ATG. Parton lab clone DVA.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    BIN1 (BIN1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-PGK-flag-RAP1B-N17-puro

    Plasmid

    #156169

    Purpose

    Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-PGK-RAP2A-WT-puro

    Plasmid

    #156170

    Purpose

    Lentiviral vector for expression of RAP2A-WT, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-PGK-RAP2A-V12-puro

    Plasmid

    #156171

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-PGK-RAP2A-N17-puro

    Plasmid

    #156172

    Purpose

    Lentiviral vector for expression of RAP2A-N17, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid

    #136657

    Purpose

    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid

    #136654

    Purpose

    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRK5-EGFP-Tau E14 P301L

    Plasmid

    #46909

    Purpose

    expresses EGFP tagged Tau E14 P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau E14 P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L AND all 14 S/P or T/P amino acid residues (…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRK5-EGFP-Tau AP P301L

    Plasmid

    #46906

    Purpose

    expresses EGFP tagged Tau AP P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau AP P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L AND all 14 S/P or T/P amino acid residues (…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK5-EGFP-Tau E14

    Plasmid

    #46907

    Purpose

    expresses EGFP tagged Tau E14 in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau E14 (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    all 14 S/P or T/P amino acid residues (T111, T153…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

    Plasmid

    #196223

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

    Plasmid

    #196224

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro

    Plasmid

    #176850

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 overexpression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full length YAP1

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

    Plasmid

    #176851

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1-5SA (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid

    #175181

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSUMO3

    Plasmid

    #52260

    Purpose

    Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-3 (SUMO3 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLKO-Tet-On-YAP1-shRNA1

    Plasmid

    #176846

    Purpose

    lentiviral vector for doxycycline-inducible human YAP1 knockdown

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    YAP1-Sh1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pINDUCER20 MEF2C

    Plasmid

    #89717

    Purpose

    Lentiviral gene expression vector for doxycycline inducible MEF2C expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLKO-Tet-On-YAP1-shRNA2

    Plasmid

    #176847

    Purpose

    lentiviral vector for doxycycline-inducible human YAP1 knockdown

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    YAP1-Sh2 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt

    Plasmid

    #175184

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v82.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt

    Plasmid

    #175180

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSUMO2

    Plasmid

    #52259

    Purpose

    Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt

    Plasmid

    #175182

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pSA1

    Plasmid

    #52261

    Purpose

    Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-1 (SUMO1 Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge