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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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19,171 results

Showing: 3801 - 3850 of 19171 results

  1. FNpCDNA3

    Plasmid
    #45346
    Depositor
    Robert Oshima
    Type
    Empty backbone
    Use
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45346-plasmid-map-sequence-id-63229

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  5. pSC101-Donor

    Plasmid
    #140630
    Purpose
    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140630-plasmid-map-sequence-id-306011

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  6. pGD-P1/HC-Pro

    Plasmid
    #196328
    Purpose
    35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    tobacco etch virus P1/HC-Pro
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196328-plasmid-map-sequence-id-390012

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  14. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46826-plasmid-map-sequence-id-246440

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  17. pMF440

    Plasmid
    #62550
    Purpose
    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
    Depositor
    Michael Franklin
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    62550-plasmid-map-sequence-id-397042

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  20. pCM66T: trimmed pCM66 backbone

    Plasmid
    #74738
    Purpose
    Small IncP-based broad host range replicating vector
    Depositor
    Mary Lidstrom
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74738-plasmid-map-sequence-id-141453

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  21. ATG-2460

    Plasmid
    #108713
    Purpose
    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt-copGFP fusion
    Use
    Lentiviral
    Tags
    CBR2opt fused to copGFP
    Expression
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    elongation factor-1
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108713-plasmid-map-sequence-id-224540

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  27. pGL3 Promoter Vector

    Plasmid
    #212939
    Purpose
    Control vector with constitutive firefly luciferase expression
    Depositor
    Debrya Groskreutz
    Insert
    luc+
    Use
    Luciferase
    Tags
    Expression
    Mutation
    WT
    Promoter
    SV40
    Available since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212939-plasmid-map-sequence-id-419192

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  28. pShuttle-CMV

    Plasmid
    #16403
    Purpose
    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only
    16403-plasmid-map-sequence-id-174093

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  35. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177939-plasmid-map-sequence-id-348111

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  40. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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  43. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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  46. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid
    #82327
    Purpose
    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)
    Depositor
    Elizabeth Stoll
    Insert
    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82327-plasmid-map-sequence-id-155713

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  49. pcDNA3/er-(n2)oxStayGold(c4)v2.0

    Plasmid
    #186296
    Purpose
    A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.
    Depositor
    Atsushi Miyawaki
    Article
    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103
    Insert
    er-(n2)oxStayGold(c4)v2.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186296-plasmid-map-sequence-id-366145

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Showing: 3801 - 3850 of 19171 results