161,273 results

1. pDONR223_DNASE2_WT

   Plasmid

   #81815

   Purpose

   Gateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DNASE2 (DNASE2 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mito-V5-APEX2

   Plasmid

   #72480

   Purpose

   Expresses APEX2 in mitochondria

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ10871

   Plasmid

   #183962

   Purpose

   U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   dCas12a(wildtype)
   poly-crRNA array targeting Sox2-Klf4-Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG and U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSF11-wmCardinal

   Plasmid

   #197251

   Purpose

   Expresses the protein of wmCardinal in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmCardinal
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCEP4-NET-myc

   Plasmid

   #107458

   Purpose

   Mammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Norepinephrine Transporter (NET) (SLC6A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR- flag-Lag16-MUC4

   Plasmid

   #205198

   Purpose

   This vector encodes of the synCAM (MUC4) and GFP nanobody LAG16

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM MUC4 and Lag16 anti-GFP nanobody
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LV_EF1a_CCNB1-P2A-Hygro_Barcode

   Plasmid

   #170218

   Purpose

   Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CCNB1 (CCNB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTHSSd_8

   Plasmid

   #59948

   Purpose

   PT7 GFP reporter

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PT7

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CL20_mEGFP_NCOA4_RET

   Plasmid

   #205865

   Purpose

   Express mEGFP-tagged fusion protein, NCOA4_RET from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NCOA4_RET (NCOA4, RET Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pN7HELIX

    Plasmid

    #190662

    Purpose

    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS4083

    Plasmid

    #131739

    Purpose

    Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

    Depositor

    Christina Smolke

    Article

    Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

    Insert

    sTRSV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AttB _EGFP

    Plasmid

    #171597

    Purpose

    Strong EGFP fluorescence from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Insert

    EGFP
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBBi-Pur GFP-TM

    Plasmid

    #139185

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.

    Depositor

    Neal Devaraj

    Insert

    GFP-TM
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    fused to membrane anchor

    Promoter

    EF1α

    Available since

    March 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-Sen1

    Plasmid

    #99994

    Purpose

    Shuttle yeast Sen1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Sen1 (SEN1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGWT35S

    Plasmid

    #182790

    Purpose

    Destination vector for transient expression in plant

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKC518 pBABE EGFP-LIN52

    Plasmid

    #192697

    Purpose

    Retroviral transgene expression of human LIN52

    Depositor

    Iain Cheeseman

    Article

    Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

    Insert

    LIN52 (LIN52 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDB-His-MBP-SARM1

    Plasmid

    #195762

    Purpose

    Purification of SARM1 from E.coli

    Depositor

    Hao Wu

    Insert

    SARM1 (SARM1 Human)
    

    Use

    Tags

    HIS-MBP-TEV

    Expression

    Bacterial

    Mutation

    truncation of first 19 residues

    Promoter

    T7

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 5aa SunTag VP64 CLV3

    Plasmid

    #115485

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTHSSd_34

    Plasmid

    #59960

    Purpose

    PTac GFP expression

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PTac

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAV hH1-ARRET

    Plasmid

    #111374

    Purpose

    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Insert

    ARRET w/o polyA
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    hH1

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAV10.F3

    Plasmid

    #63199

    Purpose

    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PLKO.1-SMG6-CDS-1

    Plasmid

    #136046

    Purpose

    SMG6 shRNA (Targeting CDS #1) inserted into the PLKO.1 plasmid (AACTTGTAAGTAACCTGCAGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    SMG6 (SMG6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PLKO.1-SMG6-CDS-2

    Plasmid

    #136047

    Purpose

    SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    SMG6 (SMG6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

    Plasmid

    #60906

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 5aa SunTag VP64 AP3

    Plasmid

    #115484

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KSR1-NanoLuc Fusion Vector

    Plasmid

    #237175

    Purpose

    Express KSR1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KSR1 (KSR1 Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. NanoBRET BiBRET Vector HaloTag-HRAS WT-BRAF RBD-NanoLuc

    Plasmid

    #236850

    Purpose

    Express HaloTag(R)-HRAS WT-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    HRAS (HRAS Human)
    BRAF (RBD) (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. GLP-1R-SmBiT Fusion Vector

    Plasmid

    #236845

    Purpose

    Express GLP-1R-SmBiT in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    GLP-1R (GLP1R Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. KSR2-HaloTag Fusion Vector

    Plasmid

    #236793

    Purpose

    Express KSR2-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KSR2 (KSR2 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. KSR1-HaloTag Fusion Vector

    Plasmid

    #236794

    Purpose

    Express KSR1-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KSR1 (KSR1 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pIDTsmart-RC2-delVP1-VP1-588TAG

    Plasmid

    #240122

    Purpose

    Express AAV2 capsid with ncAA mutant at site 588 of VP1

    Depositor

    Abhishek Chatterjee

    Article

    Pham et al bioRxiv 2024.07.20.604406

    Inserts

    RC2-DelVP1
    VP1-588-TAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Cyclin B1-Venus ND

    Plasmid

    #234673

    Purpose

    expresses a non-degradable cyclin B1 in fusion with Venus

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Inserts

    Cyclin B1 (CCNB1 Human)
    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    double mutation R42A and L45A

    Promoter

    CMV

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRCA892 - TFORF Empty Vector Puro-T2A-GFP (pRCA886 backbone)

    Plasmid

    #238180

    Purpose

    Lentiviral empty vector

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. makeTCR_Lentiviral_Backbone

    Plasmid

    #233943

    Purpose

    backbone for makeTCR assembly

    Depositor

    Michael Platten

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a (long)

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pQE-meGFP-DLC1

    Plasmid

    #192048

    Purpose

    Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

    Depositor

    Ionel Popa

    Article

    Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

    Insert

    DLC1 (DLC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHAGE-RFP-SKL

    Plasmid

    #183726

    Purpose

    For expression of RFP targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    mRFP1
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid

    #198539

    Purpose

    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTetOne-FLAG-human cGASdeltaN-HA

    Plasmid

    #186899

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pUC57 mini-ZsGreen

    Plasmid

    #222648

    Purpose

    Vector for cloning gene of interest for in vitro transcription

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    ZsGreen
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    mutated stop codon

    Promoter

    T7

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRSETA-qmTQ2-ATP

    Plasmid

    #225247

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

    Plasmid

    #194217

    Purpose

    Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    UAS-ybTATA-tagBFP; PGK-mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GAL4 UAS-ybTATA; PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pPB-cT3G-cERP2-PRDM1

    Plasmid

    #222924

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    PRDM1 (PRDM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBiT3.1-N_DCAF13:1-445

    Plasmid

    #211199

    Purpose

    Mammalian expression of full-length human DCAF13. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF13:M1-K445 (DCAF13 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    A86S annotated sequence conflict

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. mpeg:lamp2-mCherry-stop; cmlc2:GFP

    Plasmid

    #213740

    Purpose

    Expresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

    Depositor

    William Talbot

    Article

    Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

    Insert

    lamp2-mCherry
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    mpeg1.1

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. ABE-RA4.2

    Plasmid

    #208307

    Purpose

    Expresses ABE-RA4.2 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA4.2-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. ABE-RA2.0

    Plasmid

    #208299

    Purpose

    Expresses ABE-RA2.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA2.0-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. ABE-RA2.1

    Plasmid

    #208300

    Purpose

    Expresses ABE-RA2.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA2.1-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, D108G, K110R, H122R, M126I, N127K

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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