159,258 results

1. pCRISPR-PE-bacteria

   Plasmid

   #172715

   Purpose

   Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   Cas9n-linker-M-MLV2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H840A of Cas9

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Eco

   Plasmid

   #35617

   Depositor

   Patrick Salmon, Arthur Nienhuis

   Insert

   Ecotropic MLV envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLNCX2 ER:ras

   Plasmid

   #67844

   Purpose

   4-hydroxytamoxifen inducible ER:ras fusion protein

   Depositor

   Masashi Narita

   Article

   Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

   Insert

   ER1a H-RasG12V fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G525R renders the HBD largely insensitive to 17ß‐…

   Promoter

   CMV

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EZ-Tet-pLKO-Blast

   Plasmid

   #85973

   Purpose

   Tet/Dox inducible shRNA lentivirus with blasticidin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1 / TetO

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW-FIRE-pHLy

   Plasmid

   #170774

   Purpose

   Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   hUbC

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-mito-mTagBFP2

   Plasmid

   #216130

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS1C

   Plasmid

   #55168

   Purpose

   Empty vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV0286

   Plasmid

   #149333

   Purpose

   Bacterial expression of SENP_EuB protease

   Depositor

   Dirk Görlich

   Article

   Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

   Insert

   SENP_EuB protease
   

   Use

   Tags

   His14 and Tev protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid

    #180484

    Purpose

    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

    Depositor

    Julie Welburn

    Article

    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

    Insert

    CENP-E (CENPE Human, Synthetic, human)
    

    Use

    Tags

    mNeon-6xHis

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LV-RFP

    Plasmid

    #26001

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-RFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWPXL

    Plasmid

    #12257

    Purpose

    2nd generation lentiviral transfer plasmid

    Depositor

    Didier Trono

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZL-Blast FAPwt

    Plasmid

    #210643

    Purpose

    Expresses the FAP (Fibroblast Activation Protein) protein.

    Depositor

    Jing Yang

    Article

    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

    Insert

    Fibroblast Activation Protein Alpha (FAP Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOG44_FLP_recombinase (pEHA1338)

    Plasmid

    #209087

    Purpose

    FLP recombinase for FRT-mediated integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Insert

    FLP recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA GSK3 beta wt pcDNA3

    Plasmid

    #14753

    Purpose

    Mammalian expression of human GSK3 beta with HA tag

    Depositor

    Jim Woodgett

    Article

    He et al Nature. 1995 Apr 13. 374(6523):617-22.

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. LZRS-HOTAIR

    Plasmid

    #26110

    Depositor

    Howard Chang

    Article

    Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

    Insert

    HOTAIR (HOTAIR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pORANGE GFP-Camk2a KI

    Plasmid

    #131484

    Purpose

    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Use

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AiP13563 - pAAV-AiE0170h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3563)

    Plasmid

    #224029

    Purpose

    AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

    Plasmid

    #159807

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHelper_TS_V2_ampR

    Plasmid

    #214467

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    XylS / AraC

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSL1-GFP

    Plasmid

    #180420

    Purpose

    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

    Depositor

    Nancy Moran

    Article

    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-GAL9

    Plasmid

    #166689

    Purpose

    For creating a stable mCherry-GAL9 cell line

    Depositor

    Alan Sabirsh

    Article

    Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

    Insert

    LGALS9 (LGALS9 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpCas9(BB)-2A-mCherry

    Plasmid

    #197420

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge