Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 4,284 results for: TIL

Showing: 3851 - 3900 of 4284 results

  2. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid
    #135766
    Purpose
    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.
    Depositor
    Nadia Mercader Huber
    Article
    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6
    Insert
    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    UAS
    Available since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135766-plasmid-map-sequence-id-270202

    Enlarge

  3. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Article
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

    Enlarge

  16. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-260007

    Enlarge

  17. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

    Plasmid
    #73164
    Purpose
    multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells
    Depositor
    Bruce Edgar
    Article
    Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:
    Insert
    mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
    Use
    Tags
    mRFP1, GFP
    Expression
    Insect
    Mutation
    Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…
    Promoter
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    73164-plasmid-map-sequence-id-134544

    Enlarge

  21. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Samie Jaffrey
    Article
    Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    102277-plasmid-map-sequence-id-197159

    Enlarge

  23. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

    Enlarge

  26. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Tags
    Expression
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

    Enlarge

  28. pETDest

    Plasmid
    #124245
    Purpose
    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124245-plasmid-map-sequence-id-257540

    Enlarge

  29. pDOE20 mC VN

    Plasmid
    #124246
    Purpose
    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in Agrobacterium- transformed plant cells.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124246-plasmid-map-sequence-id-239323

    Enlarge

  30. pET28b_NOSER

    Plasmid
    #124244
    Purpose
    Bacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124244-plasmid-map-sequence-id-240376

    Enlarge

  31. pDOE20 mC VN transient

    Plasmid
    #124247
    Purpose
    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124247-plasmid-map-sequence-id-239326

    Enlarge

  32. pwA-ple34

    Plasmid
    #227337
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    227337-plasmid-map-sequence-id-458564

    Enlarge

  33. hOlig1-myc/pCDNA3.1

    Plasmid
    #197337
    Purpose
    Mammalian expression of an epitope-tagged hOLIG1
    Depositor
    Charles Stiles
    Insert
    OLIG1(pSM1-3) (OLIG1 Human)
    Use
    Tags
    Myc-his
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197337-plasmid-map-sequence-id-388452

    Enlarge

  34. ppG-ple12

    Plasmid
    #227339
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    227339-plasmid-map-sequence-id-458622

    Enlarge

  35. pLSU1/t35s[]:mCherry:NosT

    Plasmid
    #212177
    Purpose
    This binary vector expresses mCherry with the truncated 35sCAMV promoter and no UTR
    Depositor
    Wayne Curtis
    Insert
    mCherry
    Use
    Tags
    Expression
    Plant
    Mutation
    Truncated mCherry, missing the first 9 amino acid…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212177-plasmid-map-sequence-id-422821

    Enlarge

  36. pANY4

    Plasmid
    #164897
    Purpose
    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164897-plasmid-map-sequence-id-327986

    Enlarge

  37. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

    Enlarge

  38. StarShip01

    Plasmid
    #164505
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164505-plasmid-map-sequence-id-324722

    Enlarge

  39. StarShip02

    Plasmid
    #164506
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164506-plasmid-map-sequence-id-324723

    Enlarge

  40. StarShip03

    Plasmid
    #164507
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164507-plasmid-map-sequence-id-324724

    Enlarge

  41. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

    Enlarge

  42. pCambia-dLB-kanR-GFP

    Plasmid
    #223177
    Purpose
    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border
    Depositor
    Andreas Stuermer
    Type
    Empty backbone
    Use
    Ti plasmid for agrobacterium
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    223177-plasmid-map-sequence-id-443110

    Enlarge

  43. pLSU1/t35s[TMV]:mCherry:NosT

    Plasmid
    #212178
    Purpose
    This binary vector expresses mCherry with the truncated 35sCAMV promoter and Tomato Mosaic Virus UTR
    Depositor
    Wayne Curtis
    Insert
    mCherry
    Use
    Tags
    Expression
    Plant
    Mutation
    Truncated mCherry, missing the first 9 amino acid…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212178-plasmid-map-sequence-id-422856

    Enlarge

  44. hTLR2 flag

    Plasmid
    #13082
    Depositor
    Ruslan Medzhitov
    Insert
    TLR2 (TLR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-16 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13082-plasmid-map-sequence-id-142444

    Enlarge

  45. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

    Enlarge

  46. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P43
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

    Enlarge

  47. hTLR5 flag

    Plasmid
    #13088
    Depositor
    Ruslan Medzhitov
    Insert
    TLR5 (TLR5 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-19 have been replaced by a mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13088-plasmid-map-sequence-id-147761

    Enlarge

  50. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

    Enlarge

Showing: 3851 - 3900 of 4284 results