4,098 results

1. pLKO-p53-shRNA-427

   Plasmid

   #25636

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   p53-shRNA-427 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BE4-Gam

   Plasmid

   #100806

   Purpose

   Expresses BE4-Gam in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   BE4-Gam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pscAAV-GFP

   Plasmid

   #32396

   Depositor

   John T Gray

   Article

   Gray et al Methods Mol Biol. 2011;807:25-46.

   Insert

   GFP
   

   Use

   AAV; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmScarlet-H_C1

   Plasmid

   #85043

   Purpose

   Mammalian expression of mScarlet-H

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   mScarlet-H
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3-HA-HA-humanCMYC

   Plasmid

   #74164

   Purpose

   Mammalian expression of human cMyc

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMyc (MYC Human)
   

   Use

   Tags

   2x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-EGFP-RhoA-Q63L

   Plasmid

   #12968

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA constitutively active (RHOA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q63L Constitutively active

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-DFCP1

   Plasmid

   #86746

   Purpose

   N-terminal mCherry-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human DFCP1 (ZFYVE1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LAMP1-mRFP-FLAG

    Plasmid

    #34611

    Purpose

    3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

    Depositor

    David Sabatini

    Article

    Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    2xFlag and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag-TRIM24

    Plasmid

    #28138

    Depositor

    Michelle Barton

    Article

    Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-C1-CAMKIIalpha

    Plasmid

    #21226

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIalpha (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0001.

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC019 - LwCas13a from Leptotrichia wadei

    Plasmid

    #91909

    Purpose

    Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a and guide expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLKO.1-TSC2

    Plasmid

    #15478

    Depositor

    Do-Hyung Kim

    Article

    Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

    Insert

    TSC2 shRNA (TSC2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCytERM_mScarlet_N1

    Plasmid

    #85066

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIS2

    Plasmid

    #12177

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flag-HA-USP2

    Plasmid

    #22577

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP2 (USP2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS513 EF1alpha-cre

    Plasmid

    #11918

    Purpose

    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-HP1gamma

    Plasmid

    #17650

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 gamma (CBX3 Human)
    

    Use

    Tags

    GFP and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTriEx4-Rac1-2G

    Plasmid

    #66110

    Purpose

    Second generation Rac1 FRET biosensor

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cav1-GFP

    Plasmid

    #14433

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Caveolin-1 (CAV1 Canis familalis)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Flag-Keap1

    Plasmid

    #28023

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SIRT1 Flag

    Plasmid

    #13812

    Purpose

    Mammalian expression of human SIRT1 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    N-terminus truncated

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV H-Ras V12 IRES GFP

    Plasmid

    #18780

    Depositor

    Scott Lowe

    Insert

    H-Ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    V12

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3/Myc-DNMT3A

    Plasmid

    #35521

    Purpose

    Mammalian expression of human DNMT3A with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC034 - LwCas13a-msfGFP-2A-Blast

    Plasmid

    #91924

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFlagCMV2-EFP

    Plasmid

    #12449

    Depositor

    Dong-Er Zhang

    Article

    Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

    Insert

    EFP (TRIM25 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge