4,146 results

1. Fuw-dCas9-Tet1CD_IM

   Plasmid

   #84479

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD_IM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-HA-USP22

   Plasmid

   #22575

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP22 (USP22 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDESTmycYBX1

   Plasmid

   #19878

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Y box binding protein 1 (YBX1 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBIS

   Plasmid

   #17817

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

   Plasmid

   #29722

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mC…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-HA-COPS5

   Plasmid

   #22541

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   COPS5 (COPS5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP1

   Plasmid

   #20060

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of deletion mutant (1-43) of human…

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-Sumo1

   Plasmid

   #21154

   Purpose

   Mammalian expression of human Sumo1 with HA tag

   Depositor

   Junying Yuan

   Article

   Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

   Insert

   pcDNA3-HA-Sumo1 (SUMO1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK290 (mAID-mClover-Hygro)

   Plasmid

   #72828

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mKO2-N1

    Plasmid

    #54625

    Purpose

    Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe bleo human PPAR gamma 2

    Plasmid

    #11439

    Depositor

    Ronald Kahn

    Insert

    PPAR gamma 2 (PPARG Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-CaMPARI

    Plasmid

    #60421

    Purpose

    Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Loren Looger, Eric Schreiter

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Neo-Bam p53 R248W

    Plasmid

    #16437

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R248W (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R248W

    Promoter

    Available since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

    Plasmid

    #129719

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mEGFP

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. DsRed-rab11 DN

    Plasmid

    #12680

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    changed serine 25 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX304-Flag-APEX2-NES

    Plasmid

    #92158

    Purpose

    Cytosolic APEX2 construct

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    Flag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. moxGFP

    Plasmid

    #68070

    Purpose

    C-terminal fusion to moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Fuw-dCas9-Dnmt3a

    Plasmid

    #84476

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAX FLAG APP

    Plasmid

    #30154

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-C1-FLAG-Ku80

    Plasmid

    #46958

    Purpose

    Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku80 (XRCC5 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. IRE1 alpha-pcDNA3.EGFP

    Plasmid

    #13009

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Rictor_1 shRNA

    Plasmid

    #1853

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ChR2-2A-Halo

    Plasmid

    #22047

    Purpose

    Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

    Depositor

    Edward Boyden

    Article

    Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

    Insert

    ChR2-GFP-2A-Halo-YFP
    

    Use

    Tags

    GFP and YFP

    Expression

    Mammalian

    Mutation

    Note that there is a S147R mut in YFP. This mutat…

    Promoter

    CMV

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Tcf/Lef:H2B-GFP

    Plasmid

    #32610

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

    Insert

    Tcf/Lef:H2B-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    hsp68

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-DN-hCUL3-FLAG

    Plasmid

    #15820

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin3 (CUL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N418

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-Sec16A

    Plasmid

    #36155

    Purpose

    Expresses Sec16A as a GFP fusion

    Depositor

    David Stephens

    Article

    Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

    Insert

    Sec16A (SEC16A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mEmerald-TOMM20-N-10

    Plasmid

    #54282

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. MSCV Luciferase PGK-hygro

    Plasmid

    #18782

    Depositor

    Scott Lowe

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge