159,238 results

1. pHR_PGK_antiCD19_synNotch_Gal4VP64

   Plasmid

   #79125

   Purpose

   Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   antiCD19_synNotch_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-TRIF-CFP

   Plasmid

   #13644

   Depositor

   Doug Golenbock

   Insert

   TRIF (TICAM1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBA559

   Plasmid

   #186235

   Purpose

   pTet-LbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28 His6-mEGFP-D4H

   Plasmid

   #226373

   Purpose

   Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4H (pfoA Clostridium perfringens)
   

   Use

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. kiCAP-AAV-BI30

   Plasmid

   #183749

   Purpose

   AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   K449R, and NNSTRGG inserted between 588 and 589 o…

   Promoter

   p41

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV GFP1-9

   Plasmid

   #182244

   Purpose

   Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP 1-9 OPT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRRLsin-SV40 T antigen-IRES-mCherry

   Plasmid

   #58993

   Purpose

   lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

   Depositor

   Snorri Thorgeirsson

   Article

   Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

   Inserts

   CMV enhancer-chicken beta actin promoter
   SV40 large T antigen
   IRES-mCherry red fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   EcoRV site in MCS converted to BamHI site

   Promoter

   CMV enhancer-chicken beta actin

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Promoter

    Available since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHTN_HaloTag_KIF5B

    Plasmid

    #213404

    Purpose

    Full-length human kinesin-1 with HaloTag at the N-terminus

    Depositor

    Jonas Ries

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    HaloTag-KIF5B (KIF5B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA GFP10-ZIP

    Plasmid

    #182241

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP
    

    Use

    Tags

    split-GFP10 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA ZIP-GFP11

    Plasmid

    #182242

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP11

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    ZIP-GFP11
    

    Use

    Tags

    split-GFP11 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEM415-ldhL-mRFP1

    Plasmid

    #99842

    Purpose

    Expression of RFP in Lactobacillus

    Depositor

    Sujin Bao

    Article

    Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    ldhl

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTight-9-124-BclxL

    Plasmid

    #60857

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-MMLVgag-3xNES-ABE7.10-NG

    Plasmid

    #181753

    Purpose

    MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLVgag-3xNES-ABE7.10-NG
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BFP-KDEL

    Plasmid

    #49150

    Purpose

    visualization of the ER

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    BiP ER signal sequence (HSPA5 Human)
    

    Use

    Tags

    BFP and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, Nicholas Levinson, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge