We narrowed to 10,500 results for: fus
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pRK603
Plasmid#8831DepositorInsertTEV protease, S219D mutant
ExpressionBacterialMutationS219D mutantAvailable SinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pN1 Lck-GCaMP5G
Plasmid#34924PurposeExpresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membraneDepositorInsertLck-GCaMP5G
ExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterCMVAvailable SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAIDA1
Plasmid#79180PurposeA construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tagsDepositorInsertAdhesin Involved in Diffuse Adherence
TagsHis and MycExpressionBacterialPromoterlacUV5Available SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL-SaKKH-BE3
Plasmid#85170PurposeExpresses SaKKH-BE3 in mammalian cells [Edit]DepositorInsertSaKKH-BE3
ExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PP7_mCherry
Plasmid#61763PurposeExpresses PP7 coat protein fused to mCherryDepositorInsertPP7 coat protein fused to mCherry
UseLentiviralTagsmCherryExpressionMammalianPromoterUbc promoterAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR p53
Plasmid#12090PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression.DepositorInsertp53 shRNA (Trp53 Mouse)
UseCre/Lox, Lentiviral, and RNAiExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTH
Plasmid#131636PurposeExpresses APOBEC1 fused to the YTH domainDepositorAvailable SinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
BIC-Gag-CAS9
Plasmid#119942Purposeencodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.DepositorInsertGAG FMLV fused to spCAS9
TagsFLAGExpressionMammalianPromoterhCMVAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Stx17
Plasmid#45911DepositorAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (2M-T)
Plasmid#29708DepositorTypeEmpty backboneTags6xHis, MBP, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…PromoterEF1aAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ClopHensor
Plasmid#25938DepositorInsertClopHensor
TagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YAvailable SinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/F-tractin=mStayGold
Plasmid#212019PurposeFor filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertF-tractin
TagsmStayGoldExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-DTR-GFP
Plasmid#124364Purposeconditional expression of a diphtheria toxin receptor (DTR)–GFP fusion proteinDepositorHas ServiceAAV2InsertDTR-GFP
UseAAVTagsGFPPromoterChicken B-actinAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-scFvGCN4sfGFPTET1CD
Plasmid#82561PurposeExpression vector for antibody-sfGFP-TET1CD.DepositorInsertscFvGCN4sfGFPTET1CD
ExpressionMammalianAvailable SinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2 ER:ras
Plasmid#67844Purpose4-hydroxytamoxifen inducible ER:ras fusion proteinDepositorInsertER1a H-RasG12V fusion
ExpressionMammalianMutationG525R renders the HBD largely insensitive to 17ß‐…PromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-miniTurbo
Plasmid#124647PurposeExpresses Flag-miniTurbo fusion for proximity labelingDepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FCK-Halo-GFP
Plasmid#14750PurposeLentiviral expression of Halo-GFP for neuronal inhibitionDepositorInsertMammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
UseLentiviralTagsGFPExpressionMammalianMutationN. pharaonis halorhodopsin is mammalian codon-opt…PromoterCaMKIIaAvailable SinceApril 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AE9a
Plasmid#12433DepositorUseRetroviralTagsHAExpressionMammalianMutationAML-ETO protein with ETO exon 9a.Available SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBK-VQR-BE3
Plasmid#85171PurposeExpresses VQR-BE3 in mammalian cellsDepositorInsertVQR-BE3
ExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mito-mEosEM
Plasmid#132706Purposeapplication in Correlative light-electron microscopyDepositorInsertmitochondria targeting sequence
TagsmEosEMExpressionMammalianPromoterCMVAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
Tags6xHis, EGFP, and FLAGExpressionBacterialPromoterT7Available SinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
P210 pcDNA3
Plasmid#27481DepositorExpressionMammalianMutationContains the complete bcr/abl fusionPromoterCMVAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
TagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable SinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG153 FKBP-CFAST11
Plasmid#130813PurposeExpresses FKBP-CFAST11 in mammalian cellsDepositorInsertFKBP-CFAST11
TagsMyc-tagExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-slpGFP
Plasmid#27168DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
ExpressionBacterialAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 NusA TEV LIC cloning vector (1N)
Plasmid#29657DepositorTypeEmpty backboneTags6xHis, NusA, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-hRad51
Plasmid#125570PurposeExpresses the Rad51 protein in mammalian cells under CMV promoterDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST/LIfeAct-mCherry-N1
Plasmid#40908DepositorInsertLifeAct
TagsmCherryExpressionMammalianMutationNo stop codon to create c-terminal fusionPromoterCMVAvailable SinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianPromoterCBhAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBKCg
Plasmid#20162DepositorTypeEmpty backboneTagsGal4 DBDExpressionYeastAvailable SinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-dCas9-5xPlat2AflD
Plasmid#82560PurposeExpression vector for Cas9 peptide array (linker length: 22aa).DepositorInsertdCas9-5xPlat2AflD
ExpressionMammalianAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
psfaRb-Tn5
Plasmid#183638PurposeExpresses anti-rabbit nanobody fused with Tn5DepositorInsertanti rabbit secondary nanobody fused to Tn5
ExpressionBacterialAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc
Plasmid#21850DepositorInsertATF4 (Atf4 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationATF4 5' UTR and AUG fused to Luciferase driv…Available SinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-Stx17 WT
Plasmid#45909DepositorAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVU/RED
Plasmid#24178DepositorInsertChloramphenicol resistance gene (CmR) - ccdB gene
UseGateway Cloning and LentiviralTagsDsREDExpressionMammalianAvailable SinceSept. 23, 2010AvailabilityAcademic Institutions and Nonprofits only
