165,550 results

1. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GSN-bio-His

   Plasmid

   #52067

   Purpose

   Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   GSN (GSN Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest-676 (attR4-attR3)

   Plasmid

   #161888

   Purpose

   Multisite Gateway (attR4-attR3) vector for lentivirus production

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

   Plasmid

   #44095

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   3xFlag, 6xGly linker, ADH1 transcription terminat…

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. V72 ELP

   Plasmid

   #68938

   Purpose

   Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

   Depositor

   Andrew MacKay

   Article

   Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

   Insert

   V72 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-nSpCas9-NoABE

   Plasmid

   #209043

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS

   Plasmid

   #12158

   Depositor

   Inder Verma

   Article

   Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJiaDEST-APEX2-GAL9

   Plasmid

   #149520

   Purpose

   Express APEX2-Galectin-9 in mammalian cells

   Depositor

   Vojo Deretic

   Article

   Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

   Insert

   LGALS9 (LGALS9 Human)
   

   Tags

   APEX2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. IFI16-FL-(1-729)

   Plasmid

   #35051

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16 (IFI16 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 6, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid

    #162877

    Purpose

    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Insert consists of AA1-170

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT206

    Plasmid

    #134883

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQC V5 MCS IRES Puro

    Plasmid

    #110342

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ-CBE-SpRYn-Act3.0

    Plasmid

    #178958

    Purpose

    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE-Tn7-143

    Plasmid

    #118559

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tubp-Gal4 in pCaSpeR4

    Plasmid

    #17747

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Tags

    Tubulin 1alpha promoter and hsp70 PolyA

    Expression

    Insect

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pCMV_BE4max_P2A_GFP

    Plasmid

    #112099

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_P2A_GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGG>nls-hCas9-nls

    Plasmid

    #99138

    Purpose

    Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    humanized Cas9
    

    Use

    CRISPR

    Promoter

    Chicken beta actin (CAG)

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFL-EGFP-3xFlag-DEST

    Plasmid

    #134903

    Purpose

    Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Type

    Empty backbone

    Tags

    3x-Flag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHiUGE smFP-V5 donor (all-in-one) ORF0

    Plasmid

    #200386

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. tdTomato-Integrin-Beta1-N-18

    Plasmid

    #58105

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    Integrin-Beta1 (ITGB1 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pHCMV_JEV_prME

    Plasmid

    #207283

    Purpose

    Mammalian expression of Japanese encephalitis virus PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    JEV_PrME (POLY Japanese encephalitis virus)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHAGE-RET

    Plasmid

    #116787

    Purpose

    Lentiviral expression of RET

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RET (RET Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti-nSpCas9-APOBEC3A

    Plasmid

    #209040

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC3A-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHiUGE smFP-V5 donor (all-in-one) ORF1

    Plasmid

    #200387

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. p2CT-His-MBP-Hhe_Cas13a_WT

    Plasmid

    #91871

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    HheCas13a (HheC2c2) WT
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. mscv2.2-NLRC4

    Plasmid

    #60199

    Purpose

    Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pENTR223 CD147, open

    Plasmid

    #149720

    Purpose

    for Gateway cloning of CD147 into destination vector

    Depositor

    Anne-Claude Gingras

    Insert

    CD147 (BSG Human)
    

    Use

    Entry vector

    Mutation

    wt, without stop codon

    Available Since

    June 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pCMV GFP1-9

    Plasmid

    #182244

    Purpose

    Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP 1-9 OPT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. pET15b-17X-PTDH

    Plasmid

    #166786

    Purpose

    Phosphite dehydrogenase for NAD(P)H regeneration

    Depositor

    Wilfred van der Donk

    Article

    Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

    Insert

    Phosphite dehydroenase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A196R, T201S, A328T, E352N, and C356D- please see…

    Promoter

    T7

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. non-targeting control gRNA (BRDN0001487130)

    Plasmid

    #80262

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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33. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FLAG-D1-IRES-SS-D2-IRES-HA-D3

    Plasmid

    #215119

    Purpose

    Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Inserts

    CCND1 (CCND1 Human)
    CCND2 (CCND2 Human)
    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-tag, HA-tag, and SS (STREP-STREP)

    Expression

    Mammalian

    Promoter

    IRES and TREG3

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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35. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-TetON-CD4

    Plasmid

    #217537

    Purpose

    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    CD4 receptor (CD4 Human)
    

    Use

    AAV

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    TetOperon (TetO)

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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37. pLEX GFP-HA

    Plasmid

    #229501

    Purpose

    Control HA-tagged protein for immunoprecipitation experiments.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-TDP-43

    Plasmid

    #235487

    Purpose

    Expression of human TDP-43-EGFP

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. FUGW

    Plasmid

    #14883

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-GFP-WRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLVX-Tet3G blasticidin

    Plasmid

    #128061

    Purpose

    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge