164,577 results

1. peGFP C3-SPG20

   Plasmid

   #218578

   Purpose

   Expresses GFP-tagged human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. JD171

   Plasmid

   #229483

   Purpose

   Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E2A, VA RNA I, L4-22k/33k
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV4-ApoE2

   Plasmid

   #87085

   Purpose

   Expresses ApoE2 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E2 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PiggyBac-FLAG-hTET2-IRES-Blast

   Plasmid

   #239819

   Purpose

   PiggyBac vector to express full length human TET2 with 2X N-terminal FLAG tags

   Depositor

   Kasper Rasmussen

   Article

   Knatko et al Blood Adv. 2025 Jun 24:bloodadvances.202

   Insert

   FLAG-tagged human TET2
   

   Use

   Tags

   2x FLAG tag on TET2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xHA-TurboID-NLS_pCDNA3

   Plasmid

   #107171

   Purpose

   expresses 3xHA-tagged TurboID in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pA-His6-Tn5

   Plasmid

   #171871

   Purpose

   Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

   Depositor

   Kaiwei Liang

   Article

   Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

   Insert

   Protein A and hyperactive Tn5 transposase
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET3a-hUBA1

   Plasmid

   #63571

   Purpose

   Expression of human UBA1 tagged with His

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBA1 (UBA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Isoform 2 (1018 amino acids)

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-puro SV40 LT

   Plasmid

   #13970

   Depositor

   Thomas Roberts

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   SV40 LT (SV40gp6 )
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. SV40 1: pBSSVD2005

    Plasmid

    #21826

    Depositor

    David Ron

    Insert

    SV40 Large T (SV40gp6 simian virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    likely expresses aa109-708 of the large T antigen

    Promoter

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. hprtSAGFP

    Plasmid

    #41594

    Purpose

    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

    Insert

    SA-GFP reporter
    

    Use

    Mouse Targeting; Dsb repair reporter substrate

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_SFFV

    Plasmid

    #79121

    Purpose

    Lentiviral vector for constitutive expression of genes (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pT2/HB

    Plasmid

    #26557

    Depositor

    Perry Hackett

    Insert

    pT2/HB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV0286

    Plasmid

    #149333

    Purpose

    Bacterial expression of SENP_EuB protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuB protease
    

    Use

    Tags

    His14 and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. OpenCRISPR-1

    Plasmid

    #221565

    Purpose

    Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCas

    Plasmid

    #62225

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgR

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Has Service

    Cloning Grade DNA

    Insert

    cas9
    

    Use

    CRISPR; Lambda red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native cas9 promoter

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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