4,172 results

1. pLBH531_MBP-Cas14a1 expression

   Plasmid

   #112500

   Purpose

   Expresses MBP fused to Cas14a1

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 IKKe flag

   Plasmid

   #26201

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon (IKBKE Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBPp65ADZpUw

   Plasmid

   #26234

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   p65AD-Zip (RELA Human)
   

   Expression

   Insect

   Available Since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD104 (Peft-3::Cre)

   Plasmid

   #47551

   Purpose

   Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Insert

   Cre Recombinase
   

   Use

   Cre/Lox

   Expression

   Worm

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMVHA EED wt

   Plasmid

   #24231

   Purpose

   Mammalian expression of human EED with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EED (EED Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHaMDRwt

   Plasmid

   #10957

   Depositor

   Michael Gottesman

   Article

   Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

   Insert

   MDR1 (ABCB1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-myc3-RBBP4

   Plasmid

   #20715

   Depositor

   Yue Xiong

   Article

   He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

   Insert

   retinoblastoma binding protein 4 (RBBP4 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-fDIO EYFP

    Plasmid

    #55641

    Purpose

    Flp-Dependent EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE GFP

    Plasmid

    #10668

    Purpose

    Empty backbone for retroviral gene expression. Select recipient cells using GFP.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-U6-gRNA-CBh-mCherry

    Plasmid

    #91947

    Purpose

    Expression of empty gRNA cassette and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

    Plasmid

    #153006

    Purpose

    Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    OMM (Tom20)-sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1 Full length Importin beta

    Plasmid

    #106941

    Purpose

    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

    Insert

    human Importin beta-1 (KPNB1) (KPNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-TLR4-YFP

    Plasmid

    #13018

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 4 (TLR4 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-IRES-mCherry FP

    Plasmid

    #52114

    Purpose

    a bicistronic IRES-mCherry containing retroviral vector

    Depositor

    Dario Vignali

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. HypoxCR

    Plasmid

    #59946

    Purpose

    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

    Depositor

    Anne Le

    Article

    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

    Inserts

    GFP-Pest
    mCherry-Geminin
    

    Use

    Lentiviral

    Tags

    FLAG and PEST

    Expression

    Mammalian

    Promoter

    CMV and min CMV

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP GR

    Plasmid

    #47504

    Purpose

    Expresses glucocorticoid receptor in mammalian cells

    Depositor

    Alice Wong

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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