4,124 results

1. pCA24N-p50-ligase

   Plasmid

   #87742

   Purpose

   IPTG-inducible expression of p50-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and human p50/NF-kB (amino acids 40-366)

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTagBFP2-Lysosomes-20

   Plasmid

   #55308

   Purpose

   Localization: Lysosome Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   LAMP1
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDD162 (Peft-3::Cas9 + Empty sgRNA)

   Plasmid

   #47549

   Purpose

   Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   Empty sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EGFP-C4-Nrf2

   Plasmid

   #21549

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. ptf-Galectin3

   Plasmid

   #64149

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin3 (LGALS3 Human)
   

   Use

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Kir2.1-T2A-tdTomato

   Plasmid

   #60598

   Purpose

   Expresses Kir2.1-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiPGK Blast DEST JNKKTRmRuby2

   Plasmid

   #59154

   Purpose

   Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T1 SHP2 WT

   Plasmid

   #8322

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHIV-iRFP720-E2A-Luc

    Plasmid

    #104587

    Purpose

    Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

    Depositor

    Antonius Plagge

    Article

    Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

    Insert

    iRFP720-E2A-Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    bi-cistronic

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCS2 Flag-JunB

    Plasmid

    #29687

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    jun B oncogene (JUNB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-elavl3-H2B-GCaMP6s

    Plasmid

    #59530

    Purpose

    Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    H2B
    GCaMP6s
    

    Use

    Transposable element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Flag-IDH1

    Plasmid

    #62906

    Purpose

    Expresses wildtype IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPRyl

    Plasmid

    #70007

    Purpose

    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2015 Dec 29.

    Inserts

    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid

    #44362

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Adeno associated viral vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3/er-(n2)oxStayGold(c4)v2.0

    Plasmid

    #186296

    Purpose

    A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)v2.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge