We narrowed to 6,573 results for: PROC

1. pLostfoX-Kan

   Plasmid

   #99865

   Purpose

   TfoX induction vector for chitin-pathway transformation (KanR)

   Depositor

   Mark Mandel

   Article

   Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

   Insert

   tfoXVF
   

   Use

   V. fischeri suicide cloning vector, cmr, tcr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3

   Plasmid

   #48743

   Purpose

   Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   truncated mPer2 promoter/enhancer region (-1128 to +1060)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   truncated promoter region (see pGL6)

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

3. mCherry-Tubulin-C-18

   Plasmid

   #55148

   Purpose

   Localization: Microtubules, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. retro-gRNA-eGFP

   Plasmid

   #116926

   Purpose

   Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

   Depositor

   Diane Mathis, Christophe Benoist

   Article

   Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS_366

   Plasmid

   #182131

   Purpose

   expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

   Depositor

   George Church

   Article

   Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

   Inserts

   retron RNA
   ec.86 RT
   CspRecT
   

   Use

   Tags

   Expression

   Mutation

   incorporated rifampicin-resistance-conferring don…

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET41a iGluu

   Plasmid

   #119834

   Purpose

   Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluu
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S72T

   Promoter

   T7

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAEL005635-Cas9

   Plasmid

   #100608

   Purpose

   The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL005635-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Mutation

   Promoter

   AAEL005635 and Opie2

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-CYLD-STOP

   Plasmid

   #60077

   Purpose

   N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. En1

   Plasmid

   #34993

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Homeobox protein engrailed-1 (En1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Orai1-jGCaMP8f V203Y

    Plasmid

    #231631

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

    Insert

    Orai1-jGCaMP8f V203Y (ORAI1 Human, Synthetic)
    

    Use

    Tags

    jGCaMP8f V203Y

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEGFP-S727A-STAT3

    Plasmid

    #71446

    Purpose

    Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    S727A

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMKO.1 puro hTERT shRNA

    Plasmid

    #10688

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    hTERT shRNA (TERT Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-FLAG-KD LKB1

    Plasmid

    #8591

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K78I: lysine 78 mutated to isoleucine; kinase dead

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVT/CamKII gamma CA

    Plasmid

    #122142

    Purpose

    For making constitutively active CamKII gamma RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    constitutively active CamKII gamma (Camk2g Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    First 291 amino acid residues of CaMKII gamma

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. hMEF2A WT

    Plasmid

    #118354

    Purpose

    This plasmid expresses human MEF2A-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMKO.1 puro GFP shRNA

    Plasmid

    #10675

    Purpose

    Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    GFP shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV CMV GCGR-V5-sTurboID (N)

    Plasmid

    #153009

    Purpose

    Expresses split-TuboID N-terminal fragment fused to GCGR

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    GCGR-sTurboID (N) (L73)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. retro-gRNA-mRFP1

    Plasmid

    #112914

    Purpose

    Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

    Depositor

    Diane Mathis, Christophe Benoist

    Article

    Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

    Insert

    mRFP1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLIK-Zeo

    Plasmid

    #25736

    Purpose

    Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-nef-CIAO2-Flp

    Plasmid

    #149296

    Purpose

    Cre-dependent expression of Flp recombinase.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    Flp recombinase (flp Budding Yeast)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Derived from the mouse codon-optomized FlpO. The…

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-REVERBA-mScarlet-NLS-PEST

    Plasmid

    #190658

    Purpose

    Fluorescent reporter for circadian rhythms

    Depositor

    Mary Teruel

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

    Insert

    Reverba-mScarlet (Nr1d1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCasTet-λ

    Plasmid

    #128318

    Purpose

    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

    Depositor

    Christopher Johnston

    Article

    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

    Insert

    TetR and pTetR/TetO
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Constitutive expression of cas9 and anhydro-tetra…

    Promoter

    tet promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pet151D-TOPO ALIX Bro1-V (1-702)

    Plasmid

    #80642

    Purpose

    Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    ALIX Bro1-V (PDCD6IP Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Residues 1-702

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR-c-Met

    Plasmid

    #31786

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDACR3882 [rab-3p::HYlight::let-858utr]

    Plasmid

    #207666

    Purpose

    Expresses worm-optimized HYlight in C. elegans neurons

    Depositor

    Daniel Colón-Ramos

    Article

    Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    HYlight Biosensor
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

    Plasmid

    #187113

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSG00000101199 (ARFGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLIC-myc-GBAi W211A

    Plasmid

    #171754

    Purpose

    For the mammalian expression of myc-GBAi W211A

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pDS-RED-C1-Beclin1

    Plasmid

    #24405

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-EGFP-C1-HsDCP2

    Plasmid

    #25031

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DCP2 (DCP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge