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We narrowed to 705 results for: Bacillus

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Showing: 401 - 450 of 705 results

  4. pSP6-RNAP

    Plasmid
    #48143
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase SP6
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    V314A (numbering based on NCBI Sequence ID: NP_85…
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48143-plasmid-map-sequence-id-373044

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  7. pSLiP-G2-RacE

    Plasmid
    #188966
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA: gttagacgctgattacatggactagg
    racE
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaBAD
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188966-plasmid-map-sequence-id-371637

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  8. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Article
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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  9. SBC015869

    Plasmid
    #226281
    Purpose
    Expresses MsCAD from trc promoter; expresses BsSfp and SrCAR from a separate trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.
    Depositor
    Rainer Breitling
    Article
    Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.
    Inserts
    4'-phosphopantetheinyl transferase Sfp
    Cinnamyl alcohol dehydrogenase
    Carboxylic acid reductase
    Expression
    Bacterial
    Available Since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226281-plasmid-map-sequence-id-452183

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  12. p3R112C/T180CHFm

    Plasmid
    #85091
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G in heme-FMN domain of …
    Promoter
    T7 promoter
    Available Since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85091-plasmid-map-sequence-id-164485

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  13. pOpen-Bstpol

    Plasmid
    #165543
    Purpose
    Full length DNA polymerase from Bacillus stearothermophilus.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst DNA Polymerase, Full Length
    Use
    Synthetic Biology
    Available Since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165543-plasmid-map-sequence-id-339279

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  26. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131300-plasmid-map-sequence-id-254651

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  29. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available Since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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  37. pDR111_mannose_Stat1Klf6

    Plasmid
    #188400
    Purpose
    Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Stat-1 (Stat1 Mouse, Escherichia coli)
    Klf6 (Klf6 Mouse)
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Promoter
    D-mannose
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188400-plasmid-map-sequence-id-370468

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  38. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  39. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

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  40. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    P43
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

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  41. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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Showing: 401 - 450 of 705 results