163,606 results
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Plasmid#204497PurposeExpresses rtTA and Neomycin resistance genes in mammalian cellsDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA hEZH2
Plasmid#24230PurposeMammalian expression of human EZH2 with HA tagDepositorAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Neurospora crassa, Zebrafish, Budding Yeast)
UseZebrafish expressionTagsExpressionMutationPromoterhsp70lAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-LiLac
Plasmid#184570PurposeFluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAVDepositorInsertLiLac fluorescent sensor of lactate
UseAAVTagsHis7ExpressionMammalianMutationPromoterCAGAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti_DCLK1
Plasmid#163625PurposeExpresses DCLK1DepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV HDM2(C464A)
Plasmid#12086DepositorInsertHDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationC464A: RING-finger-domain mutantPromoterAvailable SinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
WT GBA pcDNA3.1
Plasmid#188580PurposeEctopic expression of wild-type GBADepositorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …ExpressionMutationNonePromoterEF1aAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHt2G-RFP
Plasmid#107162PurposeGolden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorTypeEmpty backboneUseFungal grobacterium transformation plasmid, teste…TagsExpressionMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1
Plasmid#59776PurposeExpresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused proteinDepositorInsertOptoFGFR1 (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-DLL1
Plasmid#205192PurposeThis vector encodes of a synCAM (DLL1) and GFPDepositorInsertsynCAM DLL1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SAC1deltaTMD-FKBP-mCherry
Plasmid#108123PurposeRecruitable cytosolic SAC1DepositorInsertSACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520)PromoterCMVAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Annexin-12
Plasmid#56583DepositorAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTT3-SP-6xHis-NTRK1(FL)-FLAG
Plasmid#157620PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorAvailable SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
Plasmid#131463PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53DepositorInsertH3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTrp53-V5, H3F3A-EGFP-AU1ExpressionMammalianMutationH3F3A G34R, Pdgfra D842VPromoternone (4x polyA to mitigate episomal expression)Available SinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hIBA1a-GFP-miR124T
Plasmid#214147PurposeAAV vector to restrict GFP expression in microglia.DepositorHas ServiceAAV5InsertGFP
UseAAVTagsExpressionMutationPromoterAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCasPA
Plasmid#113347PurposeBacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
TMPRSS15_pCSdest
Plasmid#53806DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-ZnGreen1
Plasmid#169470Purposecytosolic ZnGreen1 zinc sensor with 633 nM KdDepositorInsertZnGreen1
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPET-PKR/PPase
Plasmid#42934DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available SinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYL156 (TRV RNA2)
Plasmid#148969PurposeModified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)DepositorInsertModified TRV RNA2
UseT-dna vectorTagsExpressionMutationsee comments section belowPromoterAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG_FBXL7
Plasmid#171848PurposepcDNA3-FLAG_FBXL7DepositorInsertF-box and leucine rich repeat protein 7 (FBXL7 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBDK
Plasmid#135190PurposeIntegration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selectionDepositorInsertKanR
UseSynthetic BiologyTagsExpressionBacterialMutationPromotercatAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCNA
Plasmid#135191PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-CreI.DepositorInsertI-CreI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 Sense
Plasmid#60631PurposeExpresses the human ZKSCAN1 exon 2/3 circular RNADepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
gh38
Plasmid#106712Purposeexpression of gRNA targeting STT3ADepositorAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNK
Plasmid#135193PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Gh
Plasmid#61648PurposeGateway vector for generating green fluorescent protein fusions in fungiDepositorInsertenhanced green fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDawn
Plasmid#43796PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDOC-GG
Plasmid#149377PurposeChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene DoctoringDepositorInsertsFragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-Bsu
Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-EYFP
Plasmid#26973PurposeAAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationH134RPromoterhSynAvailable SinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV0-SMAC-HA-eGFP
Plasmid#67486PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEF-VSV-G
Plasmid#41792PurposeVSV-G-expressing envelope plasmidDepositorInsertVesicular stomatitis virus G glycoprotein, Indiana strain
UseTagsExpressionMammalianMutationPromoterCEFAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_MFSD14A
Plasmid#131869PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorAvailable SinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
plRL58
Plasmid#166886PurposeOptimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ngn1-Trpa1b-2A-mCherry
Plasmid#106425PurposeFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.DepositorInsertsUseGatewayTags2A-mCherryExpressionMutationPromoterAvailable SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNA
Plasmid#135192PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only