We narrowed to 6,909 results for: CAD
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Plasmid#19748DepositorInsertoligo targeting p53 (Trp53 Mouse)
UseCre/Lox, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAEL003877-Cas9
Plasmid#100581PurposeThe promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL003877-hspcas9-GFP
Opie-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL003877 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Zeo-TTF-1
Plasmid#75089Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorInsertTTF-1 (NKX2-1 Human)
UseRetroviralTagsnoneExpressionMammalianMutationwild-type, full-length, without native 3' UTRPromoterAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Casp8
Plasmid#48172Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of CASP8 (CASP8 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7a1 (ala)
Plasmid#14539DepositorInsertMKK7a1(Ala) (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationSer-198Ala Thr-202AlaPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
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3579 pcDNA3 Bad S136A
Plasmid#8798DepositorInsertBad (Bad Mouse)
UseTagsExpressionMammalianMutationS136APromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMyc1ElbLuc,
Plasmid#53243Purposereporter plasmid containing 1 myc cDNA binding sitesDepositorInsertpMyc1ElbLuc (MYC Human)
UseTagsExpressionMutationTo prepare plasmids with one, two, three, and fou…PromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197966PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVp and pUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIG-Flag-Sox9-IRES-nls-GFP
Plasmid#44281DepositorInsertFlag-Sox9 (SOX9 Chicken)
UseTagsFlagExpressionMammalianMutationPromoterCMV-IE enhancer and Chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-puro-3xFLAG-RUNX3 R122C
Plasmid#203439PurposeMammalian expression of RUNX4DepositorInsertRUNX3 (RUNX3 Human)
UseTags3 x FLAGExpressionMammalianMutationR122CPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308A
Plasmid#49189PurposecDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationT308APromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-SLC35B2-sgRNA
Plasmid#154860PurposeLentiviral expression of Cas9 and sgRNA targeting SLC35B2DepositorInsertSLC35B2 sgRNA (SLC35B2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBC1-PuroIII
Plasmid#153419PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(TIAM1)
Plasmid#85156Purposeacute activation of Rac through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsHisExpressionBacterialMutationE147Q, E148QPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinVenus-S1033A
Plasmid#211896PurposeVinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.DepositorInsertVinculinVenus-S1033A (VCL Chicken)
UseLentiviralTagsVenusA206KExpressionMutationmutated vinculin serine 1033 to alanine (S1033A),…PromoterCMVAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinVenus-S1033D
Plasmid#211897PurposeVinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.DepositorInsertVinculinVenus-S1033D (VCL Chicken)
UseLentiviralTagsVenusA206KExpressionMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD27h
Plasmid#73066Purposeexpression clone for human SNORD27 (C/D box snoRNA U27)DepositorInsertSNORD27 (SNORD27 Human)
UseTagsno tagExpressionMammalianMutationexpression cassette for SNORD27PromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-Luc
Plasmid#25739PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsVenusExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-GFPi
Plasmid#31848DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
SNAP-MIDAS
Plasmid#171604PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
UseTagsHis6 tag and SNAP tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
LAP2-beta-actin in modified pEGFP
Plasmid#34839DepositorInsertLAP2-beta-Actin (ACTB Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHisAlix deltaPRD
Plasmid#42577DepositorInsertdelta-PRD ALIX (residues 1-702) (PDCD6IP Human)
UseTagsHIS-TEVExpressionBacterialMutationsilent mutation A1617G (numbering in ALIX gene)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10
Plasmid#197981PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.DepositorInsertspco-nCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2-RacE
Plasmid#188966PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
racE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS1-mEGFP-NLS
Plasmid#205013PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mCherry-NLS
Plasmid#205009PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1899)
Plasmid#170143PurposeExpresses residues 1-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1900-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1547)
Plasmid#170144PurposeExpresses residues 1-1547 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1548-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (K999R)
Plasmid#170142PurposeExpresses CHD7 (K999R) in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTags6xHis and FLAGExpressionInsectMutationK999RPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
3578 pcDNA3 Bad S112A
Plasmid#8797DepositorInsertBad (Bad Mouse)
UseTagsExpressionMammalianMutationS112APromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p7612 MSCV-P C-FlagHA 16E7 E10K
Plasmid#163303PurposeExpresses HPV16 E7 E10KDepositorInsertHPV16 E7 E10K
UseRetroviralTagsFlagHAExpressionMutationE10KPromoterMSCV LTRAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-hSyn-mSyp-RT1-24xGCN4
Plasmid#106300PurposeThe plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.DepositorInsertmSyp-RT1-24xGCN4 (Syp Mouse)
UseTags24xGCN4ExpressionMammalianMutationPromoterhuman Synapsin1Available sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 (1-242)
Plasmid#24401DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 1-242 only.PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-myc-Rbx1
Plasmid#29506DepositorInsertRbx1 (Rbx1 Mouse)
UseTagsHis and mycExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/CGA-mEGFP-IRES-mCherry
Plasmid#49233PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1617 pAAV SYN1 Nuc-EYFP
Plasmid#135567PurposeAn AAV vector expressing a neuronally expressed nuclear EYFP reporterDepositorInsertsempty
Nuc-EYFP
UseAAVTags3xNLSExpressionMammalianMutationPromoterhSYN1Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIP p53
Plasmid#19745DepositorInsertoligo targeting p53 (Trp53 Mouse)
UseCre/Lox, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCsy3-VPR-Csy4
Plasmid#153943PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cellsDepositorInsertCsy3-VPR, Csy4
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCsy1-Csy2
Plasmid#153942PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cellsDepositorInsertCsy1, Csy2
UseAAV and CRISPRTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TmiR-Luc
Plasmid#25743PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S65D+T69D+S70D
Plasmid#13617DepositorUseTagsFLAGExpressionMammalianMutationchanged Serine 65, Thr 69 and Ser70 to AspPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.sgKras.9
Plasmid#91894PurposesgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.DepositorInsertKras sgRNA
UseLentiviralTagsExpressionMutationPromoterU6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/CGA-mEGFP-IRES-mCherry
Plasmid#49224PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only