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Showing: 2731 - 2760 of 167150 results

  7. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  15. pCW57.1_blasti

    Plasmid
    #194067
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194067-plasmid-map-sequence-id-380706

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  21. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid
    #160389
    Purpose
    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection
    Depositor
    John McCusker
    Insert
    autophagy-related 8 (ATG8 Budding Yeast)
    Use
    Cen/ars
    Expression
    Yeast
    Promoter
    Endogenous ATG8 promoter
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160389-plasmid-map-sequence-id-314524

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  29. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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Showing: 2731 - 2760 of 167150 results