163,606 results

1. RabV CVS-N2c(deltaG)-APEX2-Venus

   Plasmid

   #73467

   Purpose

   Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   APEX2-Venus
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQTEV-PFDN2

   Plasmid

   #31329

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   prefoldin 2 (PFDN2 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1GFP/RNase H1 D210N

   Plasmid

   #174448

   Purpose

   Express GFP-tagged RNase H1 D210N in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Use

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   D210N

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3037 GST-HPV-6 E6

   Plasmid

   #10851

   Depositor

   Peter Howley

   Article

   Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

   Insert

   HPV6b E6 (E6 Human papillomavirus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

5. IronFistMUT.

   Plasmid

   #243008

   Purpose

   Iron binding deficient IronFist

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   hemerythrin-like-mNeonGreen (FBXL5 Human)
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   H15A, H57A, E58A, E61A, H126A, E130A

   Promoter

   human phosphoglycerate kinase (hPGK)

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Iron Insensitive Control Construct 2

   Plasmid

   #243007

   Purpose

   Iron Insensitive Control Construct under the same expression promoter used in IronFist

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   mNeonGreen
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. IronFist

   Plasmid

   #243006

   Purpose

   Genetically encoded fluorescent iron reporter

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   Hemerythrin-like (FBXL5 Human)
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   human phosphoglycerate kinase (hPGK)

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-myc-RIG-I

   Plasmid

   #240814

   Purpose

   Expresses myc-tagged RIG-I in mammalian cells

   Depositor

   AJ te Velthuis

   Insert

   RIG-I (RIGI Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX208-cytoFLARE1.0-TEV

   Plasmid

   #234512

   Purpose

   Transcriptional reporter for detecting calcium increase in HEK293T (TEVp); the same TEVp involved in FLiCRE2

   Depositor

   Wenjing Wang

   Article

   Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

   Insert

   EGFP-P2A-CaM-V5-TEVp
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CCLMPC_HA-NUP98::SETBP1

    Plasmid

    #237491

    Purpose

    Express HA-tagged NUP98::SETBP1 in dual promoter lentiviral vector

    Depositor

    Charles Mullighan

    Article

    Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

    Insert

    HA-NUP98::SETBP1 (NUP98, SETBP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MND/PGK

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF.DEST51-mTurquoise2

    Plasmid

    #215539

    Purpose

    Expression vector with the mTurquoise2 fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV-hSyn-mCherry-VGAT sesRNA-2a-msFlag-2a-tTA2-WPRE

    Plasmid

    #239030

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse VGAT (Slc32a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBOBI-hPLD4-flag

    Plasmid

    #222641

    Purpose

    lentiviral vector for full length human PLD4 expression (with flag tag)

    Depositor

    David Nemazee

    Insert

    PLD4 (PLD4 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. PGK-HA-PGRN

    Plasmid

    #234872

    Purpose

    Lentiviral plasmid expressing HA-tagged human progranulin.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    Progranulin (GRN Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX208-FLiCRE2-TF

    Plasmid

    #234523

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    CD4-HA-CIBN-MK2-hLOV-TEVcs-GAL4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pIA123

    Plasmid

    #234038

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

    Depositor

    Craig Ellermeier

    Article

    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pgdh and Pxyl

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. peGFP-N1-Cofilin-S41E

    Plasmid

    #186748

    Purpose

    Expresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed serine 41 to glutamic acid

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. peGFP-N1-Cofilin-S41A

    Plasmid

    #186749

    Purpose

    Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed serine 41 to alanine

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-N1-Cofilin-S41E

    Plasmid

    #186751

    Purpose

    Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed serine 41 to glutamic acid

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNLF1-N_DCAF15:1-600

    Plasmid

    #210958

    Purpose

    Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF15:M1-L600 (DCAF15 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. Pancortin scFv [K96/7]

    Plasmid

    #190497

    Purpose

    Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-mitoX4-mVenus

    Plasmid

    #185808

    Purpose

    Mitochondrial targetted (COX8) mVenus

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    mito-mVenus
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. H2A-mTurquoise-CDC42-G12V-deltaCAAX

    Plasmid

    #176094

    Purpose

    Constitutively active, nuclear localized Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    CDC42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL4.R_TRE_minCMV

    Plasmid

    #211518

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SV40 and minCMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRE-mClover3-TDP-43ΔNLS

    Plasmid

    #214917

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Promoter

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAS9-mCherry-Frame +1

    Plasmid

    #66940

    Purpose

    CRISPaint frame selector +1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEVOL-pBpF

    Plasmid

    #31190

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

    Depositor

    Peter Schultz

    Article

    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

    Insert

    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G, E107P, D158T, I159S

    Promoter

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge