We narrowed to 30,796 results for: Eng
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Plasmid#50295DepositorInsert5'UTR, omega (Tobacco Mosaic Virus) + mitochondrial localisation signal ScCoxIV (Saccharomyces cerevisiae)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH45195
Plasmid#50275DepositorInsertpromoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH85281
Plasmid#50272DepositorInsertPromoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM5343
Plasmid#50297DepositorInsert5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT08
Plasmid#223380PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT09
Plasmid#223381PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT10
Plasmid#223382PurposeT-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpRY-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT01
Plasmid#223373PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT02
Plasmid#223374PurposeT-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT04
Plasmid#223376PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT07
Plasmid#223379PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-mir1-8mer4x (pEZ184)
Plasmid#229058PurposeDual-luciferase reporter containing 4 copies of the 8mer site of human miR-1DepositorInsertHuman miR-1 binding sites
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-HA (pKW88)
Plasmid#229046PurposeMammalian expression of CasRx-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29_anti-MERS-Nanobody-VHH-55
Plasmid#209417PurposeExpress anti-MERS-Nanobody-VHH-55 in E. coliDepositorInsertanti-mers-nanobody-vhh-55
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-MERS-Nanobody-VHH-83
Plasmid#209418Purposeexpress anti-MERS-Nanobody-VHH-83 in E. coliDepositorInsertanti-mers-nanobody-vhh-83
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-MERS-Nanobody-VHH-84
Plasmid#209419PurposeExpress anti-MERS-Nanobody-VHH-84 in E. coliDepositorInsertanti-mers-nanobody-vhh-83
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_anti-MERS-Nanobody-VHH-1
Plasmid#206890PurposeExpress anti-MERS-Nanobody-VHH-1 in E. coliDepositorInsertanti-mers-nanobody-vhh-1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOSV00157
Plasmid#222937PurposeKill switch plasmid for DNA sensor construction using Bacillus subtilisDepositorInsertTxpA-RatA toxin-antitoxin
UseTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP H2B SL2
Plasmid#227718PurposePlasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp ResistantDepositorInsertGFP H2B SL2 (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SL2 GFP H2B
Plasmid#227717PurposePlasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertSL2 GFP H2B (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SEC18-L1374
Plasmid#226275PurposePlasmid expressing the SEC18 allele from L-1374, under control of its native promoterDepositorInsertSEC18 (SEC18 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SEC18-S288C
Plasmid#226274PurposePlasmid expressing the SEC18 allele from S288C, under control of its native promoterDepositorInsertSEC18 (SEC18 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SEC18-NCYC110
Plasmid#226277PurposePlasmid expressing the SEC18 allele from NCYC110, under control of its native promoterDepositorInsertSEC18 (SEC18 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SEC18-UWOPS872421
Plasmid#226276PurposePlasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoterDepositorInsertSEC18 (SEC18 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS313-SCT1-S288C
Plasmid#226266PurposePlasmid expressing the SCT1 allele from S288C, under control of its native promoterDepositorInsertSCT1 (SCT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS313-SCT1-L1374
Plasmid#226267PurposePlasmid expressing the SCT1 allele from L-1374, under control of its native promoterDepositorInsertSCT1 (SCT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pML107-LUG1
Plasmid#226265PurposePlasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 geneDepositorInsertLUG1 gRNA (YLR352W Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pML104-LEU2-2
Plasmid#226263PurposePlasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 geneDepositorInsertLEU2 gRNA (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS313-SCT1-NCYC110
Plasmid#226269PurposePlasmid expressing the SCT1 allele from NCYC110, under control of its native promoterDepositorInsertSCT1 (SCT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS313-SCT1-UWOPS872421
Plasmid#226268PurposePlasmid expressing the SCT1 allele from UWOPS87-2421, under control of its native promoterDepositorInsertSCT1 (SCT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AraC/pBad-Tnpase (camR)
Plasmid#213909PurposeArabinose-inducible himar1C9 transposase expression - cam resistanceDepositorInsertAraC/pBad ATc-inducible himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-blast
Plasmid#210221PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_Q1310A_right
Plasmid#187849Purposeexpresses the right-side half ND6-Q1310A in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_Q1310A_left
Plasmid#187848Purposeexpresses the left-side half ND6-Q1310A in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N(Q1310A)–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
C9Y9E7
Plasmid#163230PurposeInducible expression of UniProt identifier C9Y9E7DepositorInsertC9Y9E7
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
E6SCX5
Plasmid#163275PurposeInducible expression of UniProt identifier E6SCX5DepositorInsertE6SCX5
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
F0TBY7
Plasmid#163245PurposeInducible expression of UniProt identifier F0TBY7DepositorInsertF0TBY7
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-McDAH
Plasmid#140129PurposeExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Menispermum canadense
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sox3gRNA-T1
Plasmid#127777PurposeChicken sox3 gRNA target site 1, cloned into the pU6 sgRNA (backbone #92359)DepositorInsertsox3gRNAT1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY131
Plasmid#130956PurposeEngineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Zfhx4_enh_12_Citrine
Plasmid#130597Purposeenhancer reporterDepositorInsertZfhx4_enh_12
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Zfhx4_enh_316_Cerulean
Plasmid#130598Purposeenhancer reporterDepositorInsertZfhx4_enh_316
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Lmo4_enh_15_Citrine
Plasmid#130599Purposeenhancer reporterDepositorInsertLmo4_enh_15
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Lmo4_enh_17_Citrine
Plasmid#130601Purposeenhancer reporterDepositorInsertLmo4_enh_17
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Brn3_enh_250_Citrine
Plasmid#130612Purposeenhancer reporterDepositorInsertBrn3_enh_250
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_TFAP2b_enh_32_Citrine
Plasmid#130616Purposeenhancer reporterDepositorInsertTFAP2b_enh_32
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_TFAP2b_enh_36_Citrine
Plasmid#130617Purposeenhancer reporterDepositorInsertTFAP2b_enh_36
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Cdh6_enh_318_Cerulean
Plasmid#130622Purposeenhancer reporterDepositorInsertCdh6_enh_318
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Zic1_enh_373a_Citrine
Plasmid#130623Purposeenhancer reporterDepositorInsertZic1_enh_373a
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox10_enh_89_Citrine
Plasmid#130587Purposeenhancer reporterDepositorInsertSox10_enh_89
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only