We narrowed to 18,356 results for: Tec
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Plasmid#61650PurposeGateway vector for generating yellow fluorescent protein fusions in fungiDepositorInsertenhanced yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A
Plasmid#171785PurposeExpression of HaloTag N58-(GGS)X6-hH2A in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-hH2A
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB6684
Plasmid#106154PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6631
Plasmid#106161PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKH-MoHyPer
Plasmid#78267PurposeExpress HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.DepositorInsertMoHyper
UseExpress in magnaportheTagsExpressionYeastMutationPromoterRp27Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7woH
Plasmid#65020PurposeExpresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA/Net1A
Plasmid#28262DepositorInsertNet1A (Net1 Mouse)
UseTags2x HAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPE-10u
Plasmid#13542DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionMammalianMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-1m
Plasmid#13548DepositorInsertFLIP-E A207W
UseTagsECFP and VenusExpressionMammalianMutationA207W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOTF3-AK (clone3)
Plasmid#21165DepositorInsertPOUF5F1 (genomic DNA) (POU5F1 Human)
UseCre/Lox; Homologous recombinationTagsExpressionMutationNeoR, EGFP, and SV40 polyA are between the two ge…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pBAD-eGFP
Plasmid#32553DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterpBADAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PDGFRB
Plasmid#67130PurposeStandard for cell-free expression benchmarking.DepositorInsertPDGFRB (PDGFRB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR26-2
Plasmid#104335PurposeExpresses ompR from a TetR reuglated promoter under inducible ATC control.DepositorInsertOmpR (ompR Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterPLTetO1Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dppa1-T2A-H2B-Halo donor
Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABM-D4-miR30-CypA
Plasmid#132934PurposeLentivector for CypA knockdown and blasticidin resistanceDepositorInsertCypA shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191184PurposeExpression of RPLP1 sensor in mammalian cellsDepositorInsertmCherry:RPLP1.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)
Plasmid#191175PurposeMammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).DepositorInsertCMV-TO:mCherry:s70587:EGFP (frameshifted)
UseTagsExpressionMammalianMutationEGFP is frameshiftedPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-10u
Plasmid#13546DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionMammalianMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
Plasmid#52895PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M7NS_AmpR
Plasmid#119157PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M2S_AmpR
Plasmid#119154PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192396PurposeTo act as a 2-input state of the AND gate.DepositorInsertAct2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192391PurposeTo act as a 2-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6682
Plasmid#106152PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEU3-NII-HaloLICNot
Plasmid#140185PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagsHaloExpressionMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ
Plasmid#192427PurposeTo act as the on state for testing of the alt Vika recombinase.DepositorInsertAct2::vox-OCS-vox::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192432PurposeTo act as a 2-input state of the NOR gate.DepositorInsertAct2::FRT-B3RT-Rluc-B3RT-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58
Plasmid#171431PurposeExpression of hH2A-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInserthH2A-(GGS)X6-HaloTag C58
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 SV40-AcrIIA4 (GB3333)
Plasmid#160605PurposeSV40-AcrIIA4 codon optimized for N.benthamianaDepositorInsertAcrIIA4
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLJM1-zeo-2xStrep-BFLF1
Plasmid#162661PurposeLentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cellsDepositorInsertBFLF1
UseLentiviralTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:S10-(MCS)-3xHA
Plasmid#108183PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM545-Tier1-PhCMV-Citrine
Plasmid#169505PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).DepositorInsertPCMV-driven yellow fluorescent protein citrine
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PRKCA
Plasmid#67115PurposeStandard for cell-free expression benchmarking.DepositorInsertPRKCA Protein kinase C alpha type (PRKCA Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-HD118
Plasmid#172655PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD118A/pegRNA-HD118B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACBB-TetT-LVA
Plasmid#32555DepositorInsertTetR
UseSynthetic BiologyTagsLVA tagExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPU-Empty-miR30-L1221
Plasmid#132940PurposeAll-in-one control rescue-control shRNA knockdown vectorDepositorInsertGFP
UseLentiviral and RNAiTagsHAExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPU-huTRIMmiR30-L1221
Plasmid#132937PurposeAll-in-one huTRIM5 rescue-control shRNA knockdownDepositorInserthuman TRIM5
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVH41-Tier1-PhCMV-GLuc
Plasmid#169544PurposeTier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)DepositorInsertPCMV-driven Gaussia Luciferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre
Plasmid#192421PurposeTo act as the 2-input state of the OR gate.DepositorInsertAct2::FRT-loxP-OCS-FRT-loxP::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-TAPP1-PH
Plasmid#67675PurposeMultisite gateway entry clone for adding C-terminal fusions of TAPP1-PHDepositorInsertTAPP1-PH
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEAST2-sfGFP
Plasmid#126575PurposeStrong constitutive expression of super-folder GFP for cell-free expressionDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191183PurposeExpression of PPIB sensor in mammalian cellsDepositorInsertmCherry:PPIB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA-Net1
Plasmid#28264DepositorInsertNet1 (Net1 Mouse)
UseTags2x HAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTS2374-Tier1-PhCMV-Gal4_VP16
Plasmid#169582PurposeTier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).DepositorInsertGAL4-transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#87560PurposeExpresses aa397-423 of Flp recombinase fused to PYL CIDDepositorInsertFlpO aa397-423
UseCre/Lox and Synthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#87559PurposeExpresses aa1-396 of Flp recombinase fused to ABI CIDDepositorInsertFlpO aa1-396
UseCre/Lox and Synthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationPromoterGAL1Available sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only