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Addgene

We narrowed to 31,526 results for: Eng

Showing: 4851 - 4900 of 31526 results
  1. pCgsgRNA_crtYf

    Plasmid
    #153338
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
    Depositor
    Insert
    crRNA targeting crtYf
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pYPQAT37

    Plasmid
    #223409
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.
    Depositor
    Insert
    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pYPQAT40

    Plasmid
    #223412
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.
    Depositor
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pDonor_RB-TnV2

    Plasmid
    #213907
    Purpose
    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites
    Depositor
    Insert
    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCool-mRbx1-His-hCul1-CTP

    Plasmid
    #29519
    Depositor
    Insert
    Rbx1 CUL1 (CUL1, Rbx1 Mouse, Human)
    Use
    Tags
    GST, His (before thrombin), and Thrombin Cut Site…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 26, 2011
    Availability
    Academic Institutions and Nonprofits only
  6. pGL4.R_TRE_minPro

    Plasmid
    #211517
    Purpose
    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    SV40 and minPro
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pGL4.R_TRE_minTK

    Plasmid
    #211516
    Purpose
    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    SV40 and minTK
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pZY102

    Plasmid
    #73933
    Purpose
    This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.
    Depositor
    Inserts
    bar
    GUS
    Use
    Tags
    Expression
    Plant
    Mutation
    intron is inserted into the GUS coding region
    Promoter
    35S
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pMZ3012

    Plasmid
    #204400
    Purpose
    A C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.
    Depositor
    Inserts
    quorum sensing regulator rhlR (rhlR )
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PftsH and Synthetic promoter PRhl0L0L12
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pTNSQCas

    Plasmid
    #219434
    Purpose
    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens
    Depositor
    Insert
    TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4851 - 4900 of 31526 results