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Plasmid#153338PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; SpcrDepositorInsertcrRNA targeting crtYf
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1328 LV EF1a-CD20 IRES-EGFP
Plasmid#201918Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD20DepositorInsertCD20
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT37
Plasmid#223409PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT40
Plasmid#223412PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-Hyg
Plasmid#210261PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB-p53shRNA
Plasmid#28222DepositorInsertp53 shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAC103-PBNeo-U6-sgRNA-Expression
Plasmid#48228PurposesgRNA expression on PiggyBac vector with Neo-selectable markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB
Plasmid#79050PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH6
Plasmid#129424PurposeExpression human MSH6 protein using baculovirusDepositorInserthuman MSH6 (MSH6 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF.hICN1.Ubc.GFP
Plasmid#17626DepositorInsertICN1 (NOTCH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSEZ-1
Plasmid#51868PurposeExpression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegansDepositorInsertpsdhb-1::mtLS-cpYFP
UseTagsExpressionWormMutationPromotersdhb-1Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hPMS2
Plasmid#129425PurposeExpression human PMS2 protein using baculovirusDepositorInserthuman PMS2 (PMS2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorInserthuman MLH1 (MLH1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CEPT1
Plasmid#119079PurposeVisualize localization of ER enzyme CEPT1, which converts diacylglycerol precursors to phosphatidylethanolamine or phosphatidylcholineDepositorInsertCEPT1 (CEPT1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-E1b:eGFP-Ds
Plasmid#102417PurposeFluorescent vector for screening enhancer elements in zebrafishDepositorInsertE1b:eGFP
UseTagsExpressionBacterialMutationPromoterZebrafish E1b minimal promoterAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP
Plasmid#85432PurposeLentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expressionDepositorInserthuman TRAIL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2
Plasmid#129423PurposeExpression human MSH2 protein using baculovirusDepositorInserthuman MSH2 (MSH2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH1371
Plasmid#199631PurposePrgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFPDepositorInsertGPI
UseTagsYFPExpressionWormMutationPromoterPregf-1Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCool-mRbx1-His-hCul1-CTP
Plasmid#29519DepositorUseTagsGST, His (before thrombin), and Thrombin Cut Site…ExpressionBacterialMutationPromoterAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA
Plasmid#64973PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59PromoterT7 promoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF.hHES1.Ubc.GFP
Plasmid#17624DepositorInsertHES1 (HES1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2
Plasmid#119061PurposeFluorescent vector with zebrafish pax3a enhancer 5; Tol2 versionDepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC150-PBLHL-4xHS-EF1a-DEST
Plasmid#48234PurposePiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection markerDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFW1000
Plasmid#224210PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7653_v2
Plasmid#126855Purpose7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffoldDepositorInsertII_CS3_7653_v2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-nArgBP2
Plasmid#74514PurposeExpress GFP-nArgBP2 fusion protein in mammalian cellsDepositorInsertnArgBP2 (Sorbs2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
10-4 cyclin E promoter
Plasmid#8458DepositorInserthuman cyclin E promoter (CCNE1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minPro
Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minProAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
double cut NH-donor
Plasmid#83576PurposeeGFP reporter donor containing double SA cutting sites for NHEJ knock-inDepositorInserteGFP
UseNhej donorTagsExpressionMutationPromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pFW2000
Plasmid#224211PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorTagsExpressionBacterial and MammalianMutationPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFW2100
Plasmid#224212PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minTK
Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minTKAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFPZFN1.4-B2H
Plasmid#26791DepositorInsertGFP-ZFN1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFPZFN2-B2H
Plasmid#26792DepositorInsertGFP-ZFN2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZY102
Plasmid#73933PurposeThis plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.DepositorInsertsbar
GUS
UseTagsExpressionPlantMutationintron is inserted into the GUS coding regionPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPclpBAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFW5000
Plasmid#224214PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Tol2-krt4-GFP
Plasmid#97151Purposelabel apical epithelial cells in zebrafishDepositorInsertAcGFP
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRhl0L0L12Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-subtilase A
Plasmid#35948DepositorInsertSubtilase A (subA E. Coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-rnSOX9
Plasmid#62972PurposeExpresses HA-tagged rat SOX9DepositorInsertSOX9 (Sox9 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-jGCaMP7b-XC
Plasmid#182221PurposeImproved genetic encoded calcium sensor based on GCaMP7b.DepositorInsertjGCaMP7b-XC
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTNSQCas
Plasmid#219434PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInsertTnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only