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  1. Viral Prep Service for Plasmids and Pooled Libraries

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    Collection
    ...started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...Service Addgene produces high-quality viral vector preps from select plasmids in our collection. We know ...high-titer preps are distributed as 100 µL solutions of purified viral particles that are suitable for in vivo...high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV | Browse...Request Can't find what you need in our in-stock preps? Our Packaged on Request service offers you even...eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging...
  2. AAV Packaged on Request

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    Collection
    ...service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification...protection levels. We offer 963 in-stock viral vector preps, delivering high quality and ready availability ... many researchers, scientists sometimes require preps beyond our current in-stock capabilities. To meet...start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce,...All-in-One Pricing The price for our Packaged on Request preps is all-inclusive of several important steps: MTA... varies by requesting organization Viral vector preps remain subject to the terms and conditions of the...priority. During this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes...
  3. AAV Viral Preps

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    ... AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps are purified...and viral genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified ...reported with each shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request ... pages above. You can also do a search of viral preps and filter by the serotype needed....
  4. Lentiviral Prep Service

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    Collection
    ...plasmid from us or receive a ready-to-use viral preparation from the inventory below. Lentiviruses are distributed...
  5. Biosensor AAV Preps

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    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  6. Chemogenetics AAV Preps

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    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...Sternson 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent...* Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent...
  7. Control AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  8. Optogenetics AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...table and filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's...
  9. Recombinases AAV Preps

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    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...AAV Browse the following tables for in-stock AAV preps that encode site-specific recombinases, which can...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  10. Retrograde AAV viral preps

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    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by...Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova...David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid...DREADD Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA Syn Inhibitor, Flp-dependent DREADD Gether 154868...154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA Syn Activator, Flp-dependent DREADD Gether 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell...
  11. Viral Production

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    Collection
    ...order to generate high quality preparations. After production, all virus preps are titered and subjected to...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available... was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2...Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral prep # 84445-AAVrg). Electron Microscopy The ratio of...arrowhead). Scale bar = 100 nm. Resources Browse AAV preps available from Addgene. After receiving your virus...plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation...purity and titers adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer...
  12. DNA Service - Cloning Grade DNA

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    Collection
    ...cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence has been validated by next...guarantee that Addgene will make cloning grade DNA preps of this plasmid available, but helps to inform us...
  13. Neurodegeneration Research Collection

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    ...Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral particles... Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...
  14. Genomic Deletions in Mammalian Cell Lines

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    ...a maxi-prep culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for each...colonies and inoculate into a mini-prep culture. Perform mini-prep for each sample and sequence each colony...relevant antibiotic. Select multiple colonies, mini-prep each one, and subject each clone to Sanger sequencing...oligos at a concentration of 100 μM in ddH 2 O. Prepare a 10 μl reaction mix for each guide and its reverse...using a Golden Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330 vector...Activated Cell Sorting (FACS) of Transfected Cells Prepare cells for FACS by filtering them through a 50 μm...plasmid DNA from bacterial colonies using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp...
  15. AAV Molecular Tools

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    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  16. Serotype Testing AAV

    Type
    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  17. Addgene Packaged on Request: Scope of Service

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    Collection
    ...limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service... we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons...
  18. Brain Armamentarium

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    Collection
    ...Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869... and network function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium...
  19. University of Florida Serotype Testing Panel for the Eye and Brain

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    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...sequencing of the final viral vector preparation. These viral vector preparations were produced with the pTR-UF11...Service , Addgene is producing ready-to-use viral preparations from the University of Florida for enhanced ...-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers...
  20. AAV for Neuronal Tracing

    Type
    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  21. Antibody Production

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    Collection
    ...to generate high quality preparations. Following production, all antibody preps are titered and undergo...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...repeated freeze thaw of antibodies and suggests preparing single-use aliquots before storing at -20 °C. ...
  22. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A ...
  23. CRISPR Plasmids - Epigenetics

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...
  24. CRISPR Plasmids - RNA Editing

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...
  25. Brain Initiative Collection

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    Collection
    ...Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in our... BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...the research community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN...use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...
  26. CRISPR Plasmids - RNA Targeting

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...
  27. CRISPR Plasmids - Xenopus

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...
  28. CRISPR Plasmids - Zebrafish

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...
  29. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...
  30. Optogenetics Plasmid Collection

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    Collection
    ... terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids...
  31. CRISPR Plasmids - gRNAs

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...
  32. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...
  33. Chemogenetics Plasmids

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    Collection
    ... terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics plasmids...
  34. CRISPR Plasmids and Resources

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    ...Addgene's Viral Service : Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid...
  35. CRISPR Plasmids - Base Edit

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...
  36. CRISPR Plasmids - C. elegans

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...
  37. CRISPR Plasmids - Drosophila

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...
  38. Caltech Systemic Capsids

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    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector..., Addgene is distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana...and full sequencing of the final viral vector preparation. Systemic Delivery AAV Capsid Choice Need help...: Systemic Capsids PHP.eB These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid...expression iCre AIBS , Ting PHP.S These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid...tdTomato Control Boyden PHP.V1 These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid...
  39. CRISPR Plasmids - Yeast

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...
  40. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime...
  41. CRISPR Pooled gRNA Libraries

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR... taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type...
  42. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...
Showing: 1 - 50 of 67 results