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Showing: 4001 - 4040 of 160936 results

  1. CXCR4-DuET

    Plasmid
    #213221
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213221-plasmid-map-sequence-id-430783

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  5. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

    Viral Prep
    #20298-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  6. pQCXIP-EGFP-CDMPR

    Plasmid
    #182642
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    CDMPR
    Use
    Retroviral
    Tags
    EGFP and N-terminal ER targeting sequence of hema…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182642-plasmid-map-sequence-id-358624

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  8. pGEM4Z-OMA1

    Plasmid
    #61718
    Purpose
    human Oma1 protease in the E.coli/in vitro translation vector
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pNJB91

    Plasmid
    #51519
    Purpose
    Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51519-plasmid-map-sequence-id-78693

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  13. pNJB80

    Plasmid
    #51518
    Purpose
    Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51518-plasmid-map-sequence-id-78690

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  14. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    krt1-ex1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pLenti-EGFP-SnxA

    Plasmid
    #232460
    Purpose
    Lentiviral EGFP lipid sensor for PI(3,5)P2
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-SnxA
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232460-plasmid-map-sequence-id-464306

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  22. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  23. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

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  26. pQE81XN-msYFP

    Plasmid
    #218960
    Purpose
    Expresses Hisx6-Tagged msYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    msYFP
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218960-plasmid-map-sequence-id-446223

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  27. PRS_8-GCaMP8m

    Plasmid
    #210400
    Purpose
    AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons
    Depositor
    Simon Wiegert
    Article
    Wissing et al bioRxiv 2022.01.22.477348
    Insert
    GCaMP8m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRSx8
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    210400-plasmid-map-sequence-id-415628

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  30. CXCR1-DuET

    Plasmid
    #213218
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR1 (CXCR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213218-plasmid-map-sequence-id-430780

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  31. CCR6-DuET

    Plasmid
    #213202
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR6 (CCR6 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213202-plasmid-map-sequence-id-430765

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  32. CCR3-DuET

    Plasmid
    #213199
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR3 (CCR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213199-plasmid-map-sequence-id-430762

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  33. CCR4-DuET

    Plasmid
    #213200
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR4 (CCR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213200-plasmid-map-sequence-id-430763

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Showing: 4001 - 4040 of 160936 results