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Addgene

We narrowed to 18,142 results for: Met;

Showing: 4161 - 4200 of 18142 results
  1. MERS-coV-NSP3-c001

    Plasmid
    #173085
    Purpose
    Expression of recombinant fusion protein in E. coli
    Depositor
    Insert
    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    Use
    Tags
    mCerulean
    Expression
    Bacterial
    Mutation
    D1109-D1275, codon optimized
    Promoter
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

    Plasmid
    #98932
    Purpose
    Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid
    #86610
    Purpose
    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid
    Depositor
    Insert
    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

    Plasmid
    #98931
    Purpose
    Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Insert
    cMyc Dam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. DF-UAS-sspa-GCaMP6f

    Plasmid
    #67569
    Purpose
    Zebrafish expression of a short photoactivatable fluorescent calcium reporter
    Depositor
    Insert
    sspa-GCaMP6f
    Use
    Zebrafish transgenesis (see :a spinal opsin contr…
    Tags
    Expression
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4161 - 4200 of 18142 results