We narrowed to 24,746 results for: CHI
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Plasmid#107150Purposemammalian expressionDepositorAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pBabe-GW-HA-RINT1
Plasmid#39841DepositorAvailable SinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.1
Plasmid#111066PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoterDepositorHas ServiceAAV1InsertdLight1.1
UseAAVTagsFlag tagAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Tet2.0
Plasmid#85497PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)
Plasmid#216531PurposeTargeting vector for CRISPRd generationDepositorInsertABI-dCas9-mCherry
UseCRISPRTagsmCherryExpressionMammalianPromoterTRE3GAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-ASF1B
Plasmid#31205DepositorAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4_ZV-NS5-RdRp
Plasmid#179847PurposeThe RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89DepositorInsertZika Virus Non-structural protein 5 RdRp domain
Tags6-histineExpressionBacterialAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM E274R
Plasmid#169984PurposeTet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV361_MjTyrRS
Plasmid#193250PurposeExpresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)DepositorInsertMjTyrRS
ExpressionBacterialPromoterhsp60Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
KA1042_pPB-hCMV*1-Esrrb-IV
Plasmid#124177PurposepiggyBac-based Dox-inducible expression of EsrrbDepositorAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p8328 pLIX YAP1 S94A
Plasmid#184529PurposeExpression of YAP1 S94ADepositorAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB.GFP-KIF1A tail 395-1698
Plasmid#134618PurposeMammalian expression of mouse KIF1A 395-1698DepositorInsertKIF1A (Kif1a Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 394 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF1C tail 400-1103
Plasmid#134621PurposeMammalian expression of human KIF1C 400-1103DepositorInsertKIF1C (KIF1C Human)
TagseGFPExpressionMammalianMutationWT with deletion of the 399 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-acta1:QFGal4-SV40pA
Plasmid#155121PurposeTol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertacta1:QFGal4
UseZebrafish expressionPromoteracta1Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC414 - pAAV EF1a hPREP
Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorAvailable SinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955
Plasmid#134613PurposeMammalian expression of rat KIF5C 378-955DepositorInsertKIF5C (Kif5c Rat)
TagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hASCL1 gRNA_1-MS2-Puro
Plasmid#192670PurposeLentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNA #1 (ASCL1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ACBEmax
Plasmid#139106PurposeMammalian ACBEmax expression vector (pSI-632)DepositorInsertBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
UseCRISPRExpressionMammalianPromoterCMV promoterAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-3xFLAG::Flcn-pgk-hph
Plasmid#48757PurposePiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selectionDepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_AchLightR
Plasmid#187463PurposeExpresses red acetylcholine indicator AchLightR in neuronal cellsDepositorInsertAchLightR
UseAAVTagsIgκ leaderPromoterhuman Synapsin-1Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
TagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-PD-L1 4NQ
Plasmid#121487PurposeExpression of human PD-L1 4NQ with PD-L1 shRNADepositorInsertsPD-L1 4NQ
hPD-L1 shRNA
UseLentiviralTagsFlagExpressionMammalianMutationN35Q, N192Q, N200Q and N219QPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-mEF
Plasmid#31765DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneMutationMutated in both EF-hand domains: D231A, E242K, D4…Available SinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hBTC
Plasmid#199230PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pETM30
Plasmid#85768Purposebacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
TagsGST and HisExpressionBacterialMutationcatalytic domain (aa 212-514)PromoterT7-lacAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 FL (C303S)-VC155
Plasmid#49257PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 (CASP2 Human)
TagsHA and split Venus CExpressionMammalianMutationC303SPromoterCMVAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963
Plasmid#134612PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
TagsHaloExpressionMammalianMutationWT with deletion of the 375 N-terminal residues.PromoterChicken Beta actinAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC446 - pAAV EF1a hPREP(S554A)
Plasmid#59968PurposeAn AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.DepositorAvailable SinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPCM2w/b delta6
Plasmid#47588DepositorInsertPMCA2w/b delta6 (ATP2B2 Human)
TagsEGFP-HA-HisExpressionMammalianMutation6 C-terminal AA deleted (needed for PDZ binding)PromoterCMVAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS
Plasmid#124652PurposeChimeric ETS domainDepositorAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.2
Plasmid#111069PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoterDepositorHas ServiceAAV1InsertdLight1.2
UseAAVTagsFlag tagAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
TagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDESTpK7WG-RPS5Ap-mito
Plasmid#163145PurposePlant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoterDepositorInsertRPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
ExpressionPlantAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-3xFLAG::Fnip1-pgk-hph
Plasmid#48758PurposePiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selectionDepositorAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-V5-6xHis
Plasmid#48347PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneMutationContains a stop codon at the end of the epitope t…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pEYFP_C1
Plasmid#85766Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
TagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514)PromoterCMVAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B tail 391-747
Plasmid#134615PurposeMammalian expression of mouse KIF3B 391-747DepositorInsertKIF3B (Kif5b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 390 N-terminal residues.PromoterChicken Beta actinAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-sh1-rORF
Plasmid#31764DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneMutationRNAi-resistant ORF (rORF). 8 synonymous changes i…PromoterNoneAvailable SinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBa.FRB-3myc-GS-KIF3A tail 419-702
Plasmid#134614PurposeMammalian expression of human KIF3A 419-702DepositorInsertKIF3A (KIF3A Human)
TagsFRB, 3mycExpressionMammalianMutationWT with deletion of the 418 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-6xHis-TEV-LC3BGlydelta5C
Plasmid#190237PurposePlasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_AnapRS
Plasmid#174894PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertAnapRS
ExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C tail 411-796
Plasmid#134616PurposeMammalian expression of mouse KIF3C 411-796DepositorInsertKIF3C (Kif3c Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 410 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE Tight ArchT-GFP
Plasmid#79627PurposeTetracycline-dependent expression of ArchT-EGFP in mammalian cellsDepositorInsertArchT
TagsEGFPExpressionMammalianMutationArchT is a more sensitive version of Arch (archae…PromoterTet responsive Ptight promoterAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGEX-6P1-hNF2-AR mutant
Plasmid#107149Purposebacterial expressionDepositorAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A66V
Plasmid#166817PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
NET37 pEGFP-N2 (607)
Plasmid#61990Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only