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Showing: 5401 - 5450 of 160110 results

  1. pAAV-Ef1a-fDIO mCherry (AAV Retrograde)

    Viral Prep
    #114471-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. pT4-CMV-GFP

    Plasmid
    #117046
    Purpose
    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter
    Depositor
    Wolfgang Uckert
    Insert
    GFP
    Use
    Transposon
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    117046-plasmid-map-sequence-id-258678

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  6. mApple-Alpha-V-Integrin-25

    Plasmid
    #54866
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Alpha-V Integrin (ITGAV Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54866-plasmid-map-sequence-id-330493

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  10. tdTomato-ZO1-C-14

    Plasmid
    #58147
    Purpose
    Localization: Tight Junctions, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV PHP.eB)

    Viral Prep
    #100854-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Tags
    None
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only

  22. pET30a_HIV-RT

    Plasmid
    #153311
    Purpose
    Bacterial expression of HIV reverse transcriptase
    Depositor
    Bryan Dickinson, Drew Endy
    Insert
    HIV Reverse Transcriptase
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153311-plasmid-map-sequence-id-306277

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  24. pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV2)

    Viral Prep
    #50477-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only

  26. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV1)

    Viral Prep
    #104492-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159275-plasmid-map-sequence-id-312908

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  32. pBUE411-GG

    Plasmid
    #198232
    Purpose
    Wheat GRF4-GIF1 chimera in pBUE411
    Depositor
    Andrea Gallavotti
    Insert
    GRF4-GIF1 chimera cassette
    Use
    CRISPR; Plant binary vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Ubiquitin
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198232-plasmid-map-sequence-id-394461

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  35. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  37. pcDNA3-mRFP

    Plasmid
    #13032
    Purpose
    Mammalian expression vector for cloning your gene fused to mRFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Insert
    Monomer Red Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    13032-plasmid-map-sequence-id-211651

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  43. pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)

    Viral Prep
    #100798-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Article
    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  47. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62888-plasmid-map-sequence-id-105911

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  49. MAVS-Mito

    Plasmid
    #44556
    Purpose
    Retroviral vector for expression of a chimeric MAVS allele targeted to mitochondria in mammalian cells
    Depositor
    Jonathan Kagan
    Article
    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20
    Insert
    MAVS-Fis1 chimeric protein (MAVS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    contains aa1-500 of MAVS and aa127-152 of Fis1
    Promoter
    Available since
    Aug. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5401 - 5450 of 160110 results