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166,818 results

Showing: 5401 - 5450 of 166818 results

  1. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid
    #207771
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207771-plasmid-map-sequence-id-416246

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  9. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV8)

    Viral Prep
    #20297-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available Since
    Dec. 18, 2017
    Availability
    Academic Institutions and Nonprofits only

  11. pNL [NlucP/ISRE/Hygro] Vector

    Plasmid
    #236834
    Purpose
    Express ISRE response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    ISRE
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Expression
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236834-plasmid-map-sequence-id-471835

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  19. pCAG/HA-hBIG2

    Plasmid
    #226254
    Purpose
    Expresses N-terminally HA-tagged hBIG2 in mammalian cells
    Depositor
    Hye-Won Shin
    Insert
    BIG2 (ARFGEF2 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226254-plasmid-map-sequence-id-454238

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  25. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    mRuby2
    Use
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available Since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40260-plasmid-map-sequence-id-243381

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  29. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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  31. CD47-bio-His

    Plasmid
    #53150
    Purpose
    Expresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD47 (CD47 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53150-plasmid-map-sequence-id-82772

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  34. pPolI_SARS-CoV-2_NLuc

    Plasmid
    #183953
    Purpose
    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
    Depositor
    AJ te Velthuis
    Article
    Vial et al bioRxiv 2021.06.28.450211
    Insert
    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mammalian Pol I
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183953-plasmid-map-sequence-id-365335

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  40. pV1093

    Plasmid
    #111428
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111428-plasmid-map-sequence-id-212463

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  41. AAV-CAG-flex-lck-smV5-4x6T

    Plasmid
    #196419
    Purpose
    Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smV5
    Use
    AAV and Cre/Lox; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    196419-plasmid-map-sequence-id-385571

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  42. sgRNA.SFFV.tRFP

    Plasmid
    #169941
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169941-plasmid-map-sequence-id-338357

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  43. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV1)

    Viral Prep
    #105530-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  46. p667-UBC-CDC42BPA-V5-miniTurbo_IDG-K

    Plasmid
    #189907
    Purpose
    Gateway destination clone of CDC42BPA (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDC42BPA (CDC42BPA Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    189907-plasmid-map-sequence-id-373993

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  48. pPB-CAG-Brainbow4.2-PGK-HygTK

    Plasmid
    #214126
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    Brainbow
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available Since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    214126-plasmid-map-sequence-id-428841

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  49. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TET
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

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Showing: 5401 - 5450 of 166818 results