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169,450 results

Showing: 4521 - 4560 of 169450 results

  4. pAAV-hSyn Con/Fon EYFP (AAV Retrograde)

    Viral Prep
    #55650-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  13. pYC-N

    Plasmid
    #207113
    Purpose
    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
    Depositor
    Yifan Cheng
    Article
    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    207113-plasmid-map-sequence-id-410641

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  17. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  19. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  22. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit
    #1000000229
    Purpose
    The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.
    Depositor
    Tom Ellis
    Available Since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only

  24. APEX2-OMM

    Plasmid
    #79056
    Purpose
    Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    Soybean APEX2
    Use
    Lentiviral
    Tags
    flag and last 31 amino acids of human MAVS
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79056-plasmid-map-sequence-id-238450

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  25. pAAV-mDlx-GFP-Fishell-1 (AAV PHP.eB)

    Viral Prep
    #83900-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    GFP
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only

  30. Sc RFC[hk-1s + 5]-pLANT/RIL

    Plasmid
    #239200
    Purpose
    Overeexpress Sc RFC1 (truncated N-terminus; N-terminal his tag, PKA kinase motif) in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC1 (RFC1 Budding Yeast)
    RFC5 (RFC5 Budding Yeast)
    Tags
    His-10 + PKA kinase motif on truncated Sc RFC1
    Expression
    Bacterial
    Mutation
    N-terminal truncation
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239200-plasmid-map-sequence-id-476583

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  31. Sc RFC[2+3+4]-pET

    Plasmid
    #239203
    Purpose
    Overexpress Sc RFC2, RFC3, & RFC4 in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC2 (RFC2 Budding Yeast)
    RFC3 (RFC3 Budding Yeast)
    RFC4 (RFC4 Budding Yeast)
    Expression
    Bacterial
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239203-plasmid-map-sequence-id-474660

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  32. pET28b-SUMO-SeKaiA

    Plasmid
    #229218
    Purpose
    For expression of S. elongatus KaiA protein
    Depositor
    Andy LiWang
    Insert
    kaiA (kaiA S. elongatus)
    Tags
    FLAG and SUMO
    Expression
    Bacterial
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229218-plasmid-map-sequence-id-457045

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  34. NET29B.mm pRRLSIN (1744)

    Plasmid
    #62053
    Purpose
    lentiviral expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29B (Tmem120b Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62053-plasmid-map-sequence-id-101297

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  35. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)

    Viral Prep
    #100854-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 4521 - 4560 of 169450 results