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175,655 results

Showing: 4521 - 4560 of 175655 results

  5. Adeno EA

    Plasmid
    #64071
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Insert
    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Promoter
    U6 and CBh
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. MK01

    Bacterial Strain
    #195090
    Purpose
    Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).
    Depositor
    Sander Tans
    Article
    Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1
    Bacterial Resistance
    Chloramphenicol
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid
    #87803
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87803-plasmid-map-sequence-id-175010

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  12. NPFF2-DuET

    Plasmid
    #213352
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    NPFF2 (NPFFR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213352-plasmid-map-sequence-id-430916

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  17. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts.
    Depositor
    Diego Orzaez
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pPIDCB2

    Plasmid
    #169900
    Purpose
    Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.
    Depositor
    Richard Schneider
    Article
    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05
    Type
    Empty backbone
    Use
    Avian expression
    Expression
    Mammalian
    Promoter
    Dox inducible
    Available Since
    Dec. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    169900-plasmid-map-sequence-id-334684

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  21. pPOTv7-hyg-OsAID (p5110)

    Plasmid
    #239360
    Purpose
    pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Inserts
    Rice Auxin Inducible Degradation domain
    Auxin Inducible Degradation domain
    Use
    Bacterial
    Promoter
    none
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239360-plasmid-map-sequence-id-478808

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  22. pPOTv6-puro-3Ty:OsAID:3Ty (p5229)

    Plasmid
    #239361
    Purpose
    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Insert
    Rice Auxin inducible degradation domain
    Use
    Bacterial
    Tags
    3 x Ty epitope tag
    Promoter
    none
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239361-plasmid-map-sequence-id-478802

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  23. pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)

    Plasmid
    #239362
    Purpose
    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Insert
    Rice Auxin inducible degradation domain
    Use
    Bacterial
    Tags
    3 x Ty epitope tag
    Promoter
    n/a
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239362-plasmid-map-sequence-id-478759

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  26. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV PHP.eB)

    Viral Prep
    #44362-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    March 22, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pDB-His-MBP-SARM1

    Plasmid
    #195762
    Purpose
    Purification of SARM1 from E.coli
    Depositor
    Hao Wu
    Insert
    SARM1 (SARM1 Human)
    Tags
    HIS-MBP-TEV
    Expression
    Bacterial
    Mutation
    truncation of first 19 residues
    Promoter
    T7
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195762-plasmid-map-sequence-id-384751

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  37. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100936-plasmid-map-sequence-id-206124

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  39. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129593-plasmid-map-sequence-id-254271

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Showing: 4521 - 4560 of 175655 results