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Showing: 441 - 480 of 2723 results

  1. pCDH-EF1S-copGFP

    Plasmid
    #73034
    Purpose
    Express copGFP reporter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73034-plasmid-map-sequence-id-133977

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  4. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

    Plasmid
    #202568
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202568-plasmid-map-sequence-id-429225

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  5. HRE:GFP-pGK:Cre

    Plasmid
    #68552
    Purpose
    Lentivirus expressing hypoxia-responsive GFP reporter and Cre
    Depositor
    Tyler Jacks
    Inserts
    Green Fluorescent Protein
    Cre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HRE and pGK
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    68552-plasmid-map-sequence-id-120714

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  7. BII-tdT-inv-GFP

    Plasmid
    #133393
    Purpose
    Piggybac vector for bidirectional promoter reporter assay
    Depositor
    Blair Madison
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    No promoter
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133393-plasmid-map-sequence-id-268036

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  9. INS-GFP-CD19-EPT

    Plasmid
    #210469
    Purpose
    Donor plasmid for knock-in GFP-CD19 into the human INS locus
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210469-plasmid-map-sequence-id-419052

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  11. EGFP-uTEV1-MAVS-mTA2

    Plasmid
    #171000
    Purpose
    HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[R537A, R538A]
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[R537A, R538A]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171000-plasmid-map-sequence-id-335835

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  12. EGFP-uTEV1-MAVS-mTA5

    Plasmid
    #171003
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI, R537A]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171003-plasmid-map-sequence-id-335793

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  13. EGFP-uTEV1-MAVS-mTA6

    Plasmid
    #171004
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171004-plasmid-map-sequence-id-335832

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  16. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Article
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Use
    Tags
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverse…
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61628-plasmid-map-sequence-id-396550

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  25. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Article
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Insert
    DD-dCas9VP192-T2A-EGFP
    Use
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69534-plasmid-map-sequence-id-122831

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  26. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  27. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154770
    Purpose
    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, G1 PylT with A41AA and C5…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154770-plasmid-map-sequence-id-309867

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  29. pCS2+MCS-P2A-sfGFP

    Plasmid
    #74668
    Purpose
    Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.
    Depositor
    Jason Berman
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74668-plasmid-map-sequence-id-146800

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  30. 1203A-CMV-5’DR-EGFP-bGHpolyA

    Plasmid
    #221452
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221452-plasmid-map-sequence-id-437434

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  31. 1203B-CMV-3’DR-EGFP-bGHpolyA

    Plasmid
    #221453
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221453-plasmid-map-sequence-id-437437

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  32. M38 TOP-dGFP

    Plasmid
    #17114
    Depositor
    Randall Moon
    Insert
    Tcf/Lef sites (Ctnnb1 )
    Use
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    17114-plasmid-map-sequence-id-241972

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  33. AICSDP-34: TNNI1-mEGFP

    Plasmid
    #114411
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114411-plasmid-map-sequence-id-220877

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  34. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99375-plasmid-map-sequence-id-191025

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Showing: 441 - 480 of 2723 results