Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,330 results for: Ank

Clear keywords
Showing: 6401 - 6450 of 7330 results

  3. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

    Enlarge

  5. pNvT-MHC::mCh

    Plasmid
    #67943
    Purpose
    Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
    Depositor
    Ulrich Technau
    Article
    Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107
    Insert
    mCherry
    Use
    Transgenic reporter in the sea anemone, nematoste…
    Promoter
    Myosin Heavy Chain1 (MyHC1)
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67943-plasmid-map-sequence-id-266110

    Enlarge

  8. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

    Enlarge

  11. pHeACT-mEGFP-NLS

    Plasmid
    #205003
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    Use
    Tardigrade expression
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205003-plasmid-map-sequence-id-405176

    Enlarge

  12. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

    Enlarge

  13. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Yeast
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

    Enlarge

  18. pCB-2

    Plasmid
    #210388
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210388-plasmid-map-sequence-id-418474

    Enlarge

  20. pMBEC6

    Plasmid
    #187598
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187598-plasmid-map-sequence-id-367542

    Enlarge

  22. pC5Kan-P2A

    Plasmid
    #51814
    Purpose
    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message
    Depositor
    Barry Ganetzky
    Article
    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:
    Insert
    P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
    Use
    Shuttle vector for insect expression vectors
    Mutation
    codons altered for Drosophila and to eliminate re…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51814-plasmid-map-sequence-id-79554

    Enlarge

  23. pPEE47

    Plasmid
    #139426
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mNeonGreen
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    139426-plasmid-map-sequence-id-297721

    Enlarge

  24. pPEE46

    Plasmid
    #139425
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mRuby3
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    139425-plasmid-map-sequence-id-292473

    Enlarge

  25. QUIN

    Plasmid
    #184267
    Purpose
    A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.
    Depositor
    Feng Qu
    Article
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA1 cDNA with 4 introns
    Expression
    Plant
    Mutation
    S1098P, K1651R
    Promoter
    CaMV 35S with duplicated enhancer
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184267-plasmid-map-sequence-id-363074

    Enlarge

  26. pHeEf1a-mEGFP-NLS

    Plasmid
    #205012
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
    Use
    Tardigrade expression
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205012-plasmid-map-sequence-id-405256

    Enlarge

  31. pENTR-AtMIR390a-B/c

    Plasmid
    #51778
    Purpose
    Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Gateway entry vector
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51778-plasmid-map-sequence-id-79411

    Enlarge

  34. pMBEC2

    Plasmid
    #187596
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187596-plasmid-map-sequence-id-367535

    Enlarge

  36. pRvTUB-mEGFP-NLS

    Plasmid
    #205011
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
    Use
    Tardigrade expression
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205011-plasmid-map-sequence-id-405254

    Enlarge

  40. pH3mCHSH2

    Plasmid
    #101053
    Purpose
    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.
    Depositor
    Jennifer Lodge
    Article
    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108
    Insert
    Cryptococcus mCherry expression plasmid
    Expression
    Yeast
    Promoter
    Cryptococcus Histone3 promoter
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    101053-plasmid-map-sequence-id-192942

    Enlarge

  42. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227300-plasmid-map-sequence-id-451538

    Enlarge

  43. pRvSAHS1-mEGFP-NLS

    Plasmid
    #205005
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205005-plasmid-map-sequence-id-405182

    Enlarge

  44. pRvCAHS3-mEGFP-NLS

    Plasmid
    #205004
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205004-plasmid-map-sequence-id-405181

    Enlarge

  47. p3015b

    Plasmid
    #209317
    Purpose
    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.
    Depositor
    Thomas Hooven
    Article
    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1
    Insert
    Group B Streptococcus-compatible single guide RNA
    Expression
    Bacterial
    Promoter
    Xyl/tet promoter that is constitutively active
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    209317-plasmid-map-sequence-id-435309

    Enlarge

  48. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

    Enlarge

Showing: 6401 - 6450 of 7330 results