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178,225 results

Showing: 7551 - 7600 of 178225 results

  1. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  2. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV2)

    Viral Prep
    #100054-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CAG
    Tags
    mCherry
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  3. pMH-Halo tag

    Plasmid
    #154144
    Purpose
    Gateway compatible mammalian expression plasmid for halo tag fusion proteins
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    Halo tag
    Expression
    Mammalian
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154144-plasmid-map-sequence-id-309445

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  4. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

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  5. AAV SYN PSAM4 GlyR IRES EGFP (AAV5)

    Viral Prep
    #119742-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV SYN PSAM4 GlyR IRES EGFP (#119742). In addition to the viral particles, you will also receive purified AAV SYN PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Scott Sternson
    Article
    Magnus et al Science. 2019 Mar 14. pii: science.aav52
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (AAV1)

    Viral Prep
    #84445-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (#84445). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] plasmid DNA. CAG-driven, Cre-dependent Jaws-GFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    CAG
    Tags
    GFP (Cre-dependent)
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only

  22. pHR-hSyn-EGFP

    Plasmid
    #114215
    Purpose
    Lentiviral vector (control) that targets expression of EGFP to neurons
    Depositor
    Xue Han
    Article
    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral and Mouse Targeting
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
    Promoter
    human synapsin (hSyn)
    Available Since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    114215-plasmid-map-sequence-id-223405

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  23. pCLX-PGK-hTERT

    Plasmid
    #114315
    Purpose
    Constitutive expression of the hTert protein
    Depositor
    Patrick Salmon
    Insert
    hTERT (TERT Human)
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114315-plasmid-map-sequence-id-218852

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  24. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Available Since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    99698-plasmid-map-sequence-id-194602

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  25. sAJM.1506

    Bacterial Strain
    #108254
    Purpose
    Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655
    Depositor
    Christopher Voigt
    Article
    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038
    Bacterial Resistance
    Chloramphenicol
    Available Since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)

    Viral Prep
    #68717-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#68717). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. CAG-driven, Cre dependent, GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Promoter
    CAG-FLEX
    Tags
    mRuby2 (Cre-dependent)
    Available Since
    May 4, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. pAAV.CAG.GCaMP6s.WPRE.SV40 (AAV1)

    Viral Prep
    #100844-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.GCaMP6s.WPRE.SV40 (#100844). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6s.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  33. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  34. pSNAPf-ADRβ2 Control Plasmid

    Plasmid
    #101123
    Purpose
    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    Beta-2-Adrenergic Receptor (ADRB2 Human)
    Tags
    SNAP-tag (SNAPf)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101123-plasmid-map-sequence-id-194092

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  36. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  39. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  40. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

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  45. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

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Showing: 7551 - 7600 of 178225 results