Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,244 results for: sgRNA

Clear keywords
Showing: 361 - 380 of 9244 results

  1. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

    Enlarge

  2. Multiple Lentiviral Expression (MuLE) system

    Plasmid kit
    #1000000060
    Purpose
    A plasmid toolbox used to construct vectors for constitutive or inducible gene expression, knockdown, deletion or editing.
    Depositor
    Ian Frew
    Application
    Cloning and Synthetic Biology
    Vector type
    Lentiviral, Mammalian Expression
    Cloning type
    MultiSite Gateway
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. Intron-Tagging-EGFP-Donor

    Plasmid
    #159740
    Purpose
    Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.
    Depositor
    Stefan Kubicek
    Publication
    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Use
    Intron tagging donor
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159740-plasmid-map-sequence-id-314864

    Enlarge

  4. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

    Enlarge

  6. pX330 Ctnnb1.1

    Plasmid
    #59911
    Purpose
    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    59911-plasmid-map-sequence-id-123316

    Enlarge

  7. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

    Plasmid
    #114729
    Purpose
    3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene
    Depositor
    Martine Roussel
    Publication
    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA M5, sgRNA M7, sgRNA M9
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114729-plasmid-map-sequence-id-222610

    Enlarge

  8. pYC1640

    Plasmid
    #158719
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Publication
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158719-plasmid-map-sequence-id-320840

    Enlarge

  12. pPEPX-P3-sgRNAluc

    Plasmid
    #85590
    Purpose
    Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for
    Depositor
    Jan-Willem Veening
    Publication
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    sgRNA targeting firefly luciferase encoding gene
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85590-plasmid-map-sequence-id-169513

    Enlarge

  20. pCFD4d-U6-1:white1-U6-3:white1

    Plasmid
    #84005
    Purpose
    pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter
    Depositor
    Phillip Zamore
    Publication
    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    white sgRNA-1
    white sgRNA-1
    Use
    CRISPR
    Promoter
    U6:1, U6:3, and dU6-1; dU6-3
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84005-plasmid-map-sequence-id-160505

    Enlarge

Showing: 361 - 380 of 9244 results