We narrowed to 18,777 results for: IRE

1. pCDNA3.1 AT1AR-TSTS/4A

   Plasmid

   #45634

   Depositor

   Robert Lefkowitz

   Article

   Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

   Insert

   AT1AR-TSTS/A (Agtr1a Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Thr332, Ser335, Thr336, and Ser338 all changed to…

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

2. GFP-MYO10-BioID

   Plasmid

   #194858

   Purpose

   Express MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   MYO10 (MYO10 Human)
   

   Use

   Tags

   BioID and EGFP

   Expression

   Mammalian

   Mutation

   MyoX contains a Q680R variant compared to the NCB…

   Promoter

   CMV

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMV epArcLight

   Plasmid

   #85803

   Purpose

   genetically-encoded fluorescent voltage indicator

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   epArcLight
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; ecliptic pHluorin…

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDsRed2-C1-Ahi1

   Plasmid

   #30495

   Depositor

   Joseph Gleeson

   Article

   Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

   Insert

   Ahi1 (AHI1 Human)
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

5. ET1-human LRRC33

   Plasmid

   #111603

   Purpose

   Express human LRRC33 in 293T cells

   Depositor

   Timothy Springer

   Article

   Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

   Insert

   human LRRC33 (NRROS Human)
   

   Use

   Tags

   PreScission/TEV/ProteinC/6xHIS/SBP/

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pminiT-Halo-MED1 donor

   Plasmid

   #197868

   Purpose

   Donor plasmid used for building Halo-MED1 knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   CRSP210 (Med1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Halotag

   Expression

   Mutation

   Promoter

   NA

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBacDual with LFn + PrgJ

   Plasmid

   #84869

   Purpose

   Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   PrgJ
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5FRT/TO-UBXD1-Strep-HA

   Plasmid

   #113476

   Purpose

   Expression of human UBXD1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD1 (UBXN6 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   C174T silent mutation, wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST_DHN_AtCor47 (pBS0784)

   Plasmid

   #185254

   Purpose

   For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DHN_AtCor47
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. cARA12-mRFP

    Plasmid

    #181961

    Purpose

    ARA12 protein tagged with mRFP, under control of TBA2 promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    ARA12 (ARA12 Mustard Weed)
    

    Use

    Tags

    mRFP

    Expression

    Plant

    Mutation

    Promoter

    TBA2

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TNPO1-shRNA

    Plasmid

    #86082

    Purpose

    A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-Map7-Start-1351

    Plasmid

    #46075

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-Start-1351 (Map7 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWZL Blast H-Ras G12V E37G

    Plasmid

    #12277

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V E37G (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The E37G mutat…

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pWZL Blast H-Ras G12V Y40C

    Plasmid

    #12279

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pSL0721 (pDonor_L1-105)

    Plasmid

    #130645

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short L)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30Xa-SBL1(160S)

    Plasmid

    #66872

    Purpose

    Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
    

    Use

    Tags

    Histag from pET30Xa-LIC

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pK-SAN1-Flag

    Plasmid

    #117163

    Purpose

    Expresses Flag-tagged SAN1 nuclease in mammalian cells.

    Depositor

    Ian Macara

    Article

    Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

    Insert

    SAN1 (FAM120B Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 432 pSG5L HA RB 567L

    Plasmid

    #10725

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB 567L (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    567L

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-3xFLAG-Puro PAK5 K478R K469R

    Plasmid

    #167180

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R K469R
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFUW-TetO-Txnip

    Plasmid

    #178437

    Purpose

    doxycycline-inducible expression of Mouse Txnip in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Txnip (Txnip Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSL0376 (His-MBP-StrepII-TniQ)

    Plasmid

    #130641

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite-StrepII on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LLP250 pEF1a-TALE-SunTag

    Plasmid

    #100939

    Purpose

    Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMT2-MEKK3-K392A

    Plasmid

    #21585

    Depositor

    John Kyriakis

    Article

    Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

    Insert

    mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lysine 392 to Arginine

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pSL0715 (pDonor_R1-57)

    Plasmid

    #130644

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short R)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GST-PIWI(aa478-860)

    Plasmid

    #21542

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    contain only aa478-860

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pLJC2-UMPS (Y37A, R155A)-3xFLAG

    Plasmid

    #87976

    Purpose

    Expression of UMPS (Y37A, R155A) in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    UMPS (Y37A, R155A) (UMPS Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y37A, R155A

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLJC2-Rap2A-3xFLAG

    Plasmid

    #87974

    Purpose

    3rd generation lentiviral vector for Expression of Rap2A in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAA3

    Plasmid

    #154163

    Purpose

    Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    20 wt NF-κB sites were replaced with Rel1A-specif…

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX330-sgRNA_Dicer_3

    Plasmid

    #68809

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

    Plasmid

    #146553

    Purpose

    Mammalian Expression of HsSmg6-del36-576-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsSmg6-del36-576-siRNAres (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSECB sgMTHFD2L_1

    Plasmid

    #106306

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2L (MTHFD2L Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSECB sgMTHFD2L_2

    Plasmid

    #106307

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2L (MTHFD2L Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 33-G-X1-pEF1-puro

    Plasmid

    #111604

    Purpose

    Express human 33-G-X1 in 293T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    human LRRC33 and GARP chimeria (NRROS Human)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

    Plasmid

    #159658

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. XE157 GFP-XDpr-delta PDZB-CS2+

    Plasmid

    #16765

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    XDpr-delta PDZB (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    gfp

    Expression

    Mutation

    XDpr is truncated to delete the last four amino a…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pCDNA GAL4 CREB 1-341 M1

    Plasmid

    #22973

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

    Insert

    CREB (Creb1 Rat, GAL4-fusion)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine of CREB

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL2-basic mt E2F-luciferase

    Plasmid

    #133708

    Purpose

    Expression of E2F1-mt-luc.

    Depositor

    James A. DeCaprio

    Article

    Zalvide et al Mol Cell Biol 1998 Mar;18(3):1408-15.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Trxn factor bind site

    Tags

    Expression

    Mammalian

    Mutation

    the three binding site copies are mutated form

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 360 pCR kdrb

    Plasmid

    #13067

    Depositor

    Nathan Lawson

    Article

    Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

    Insert

    kdrb (kdr Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pCas9GG

    Plasmid

    #131009

    Purpose

    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_R0040 TetR

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pX330-sgRNA_Dicer_2

    Plasmid

    #68808

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3-HA-CUL1 L756A/I757A

    Plasmid

    #19938

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 L756A/I757A (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutation of two adjacent hydrophobic residues, L…

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

44. pY036_ATP1A1_G5

    Plasmid

    #86618

    Purpose

    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

    Plasmid

    #111155

    Purpose

    knock in bait for Axolotl Tyrosinase locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

    Insert

    Tyrosinase-bait-T2a-Cherry-pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pJS278

    Plasmid

    #134139

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255, R155A

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pVMG0173_Cq-Actin5c-Cas9

    Plasmid

    #169345

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0173_Cq-Actin5c-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ009808

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. L309-myc-seipin-CT

    Plasmid

    #64063

    Purpose

    myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Contains Arg326 to Ser443

    Promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pCSP1-mp53AS

    Plasmid

    #20896

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

    Insert

    p53AS (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' natural EcoRV and SacI restriction sites …

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

50. pCRII-Sema4a-ISH-2kb

    Plasmid

    #68029

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4A (Sema4a Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    a 2kb cDNA fragment for ISH probe synthesis

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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