225,398 results
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Lenti-miniTurbo-NLS-Blast
Plasmid#232347PurposeLentiviral control vector expressing miniTurboNLS without dCas9DepositorInsertminiTurbo
UseLentiviralTags3xHA and 3xSV40 NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationN346A-C348APromoteraraBADAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-N1
Plasmid#54642PurposeLocalization: N1 Cloning Vector, Excitation: 554, Emission: 581DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-N1
Plasmid#54642PurposeLocalization: N1 Cloning Vector, Excitation: 554, Emission: 581DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-alpha-synuclein
Plasmid#51486PurposeInducible expression of human alpha-synuclein in E.coliDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a-alpha-synuclein
Plasmid#51486PurposeInducible expression of human alpha-synuclein in E.coliDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-2A-MCS2
Plasmid#71782PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-2A-MCS2
Plasmid#71782PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
XEC
Plasmid#233341Purposeexpressing SARS-CoV-2 XEC spike protein for pseudovirus productionDepositorInsertSpike (XEC) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationT22N, F59S on the basis of KP.3PromoterCMVAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
KP.3.1.1
Plasmid#233342Purposeexpressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus productionDepositorInsertSpike (KP.3.1.1) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationS31del on the basis of KP.3PromoterCMVAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1
Plasmid#121492PurposeExpression of human PD-L1 WT in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003
Plasmid#133447PurposeExpresses SpyCatcher003 in bacterial cytoplasmDepositorInsertSpyCatcher003
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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EcNR2 Strain
Bacterial Strain#26931DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pExp-GB1
Plasmid#112565PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-GB1 and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-V5-TurboID
Plasmid#166971PurposeExpresses ER targeted TurboID for secretome biotinylationDepositorInsertSec61b-V5-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFYF1320 EGFP Site#1
Plasmid#47511Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFYF1320 EGFP Site#1
Plasmid#47511Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCF159_BRL
Plasmid#225962PurposeCMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.DepositorInsertCMV-Intron-BRL (env protein)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pExp-NusA
Plasmid#112571PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-1P-Kv-WPRE
Plasmid#202612PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSynDepositorInsertJEDI-1P-Kv
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pHluorin_M153R-CD63-mScarlet
Plasmid#172118PurposeDual-color fluorescent reporter for CD63-positive exosome secretion and uptakeDepositorInsertCD63 (CD63 Human)
UseLentiviralTagsmScarlet and pHluorin_M153R in a small extracellu…ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-v2-TC
Plasmid#194326PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PyronicSF/pcDNA3.1(-)
Plasmid#124812PurposeHighly resposive GFP-based pyruvate nanosensor.DepositorInsertPyronicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP670
Plasmid#136571PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertH2B-emiRFP670
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 LB.1
Plasmid#223277PurposeEncodes SARS-CoV-2 spike LB.1 for pseudovirus productionDepositorInsertSARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
UseTagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-29: LMNB1-mTagRFP-T
Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
LBR-mCherry
Plasmid#172446PurposeExpression of nuclear envelope protein LBR with a C-terminal mCherry tagDepositorInsertlamin B receptor (LBR Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human IFN-gamma promoter/enhancer
Plasmid#17598DepositorInsertIFN gamma promoter with 1st intron enhancer (IFNG Human)
UseTagsluciferaseExpressionMammalianMutation-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
Human IFN-gamma promoter/enhancer
Plasmid#17598DepositorInsertIFN gamma promoter with 1st intron enhancer (IFNG Human)
UseTagsluciferaseExpressionMammalianMutation-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro
Plasmid#227256PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
Plasmid#160540PurposeExpresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.DepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV9)
Viral Prep#162378-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFr.WPRE.SV40
Plasmid#98929PurposeAAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFr.WPRE.SV40
Plasmid#98929PurposeAAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterArabidopsis ubiquitin 10 (AtUbi10)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterArabidopsis ubiquitin 10 (AtUbi10)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-EGFP-ADRP
Plasmid#87157PurposeFluorescent protein localized to lipid droplets (LDs)DepositorInsertADRP (PLIN2 Human)
UseTagsEGFPExpressionMammalianMutationPromotermCherry-R TTGGTCACCTTCAGCTTGGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-EGFP-ADRP
Plasmid#87157PurposeFluorescent protein localized to lipid droplets (LDs)DepositorInsertADRP (PLIN2 Human)
UseTagsEGFPExpressionMammalianMutationPromotermCherry-R TTGGTCACCTTCAGCTTGGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
G5-CD40
Plasmid#121166PurposepMP71 vector expressing GFP - P2A - G5 - myc - CD40DepositorInsertG5-CD40
UseRetroviralTagsGFP and mycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHM6-alphasynuclein-A53T
Plasmid#40825DepositorInsertSNCA (SNCA Human)
UseTags6x His and HAExpressionMammalianMutationA53TPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only
