We narrowed to 60,538 results for: Gene
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Plasmid#72164PurposeExpresses the extracellular region of the Sema6A, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCA-G-intron(Neo)-T(FLLFLNL)
Plasmid#40020DepositorInsertsGFP
tdT-3Myc
beta-globin intron
Neo
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR 2xU6:gRNA, mitfa:Cas9
Plasmid#118844PurposeTargets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorTypeEmpty backboneUseCRISPR; Tol2Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2518
Plasmid#91075PurposeModule B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRPromoterTaU6Available SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hygro-iDEST
Plasmid#75339PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
ExpressionMammalianPromoterTRE-tightAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
4xHRE-MinTK-CRE-ODD
Plasmid#141147Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsert4xHRE-MinTK-CRE-ODD
UseLentiviralMutationcontains an altered Cre gene modified by the addi…Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…ExpressionMammalianAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human Lentiviral sgRNA Library - Ribosomal Proteins
Pooled Library#51045PurposeSub-pool of Sabatini/Lander sgRNA library enriched for ribosomal gene targets.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS2-123
Plasmid#26598DepositorInsertSynthetic
ExpressionBacterialMutationN/AAvailable SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorMutationD10A, H840APromoterPj23119 and ptbAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MVP_WT_V5
Plasmid#82977PurposeGateway Donor vector containing MVP, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Syn61Δ3(ev5) ΔrecA (ev1)
Bacterial Strain#189857PurposeLaboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.DepositorBacterial ResistanceStreptomycinSpeciesSyntheticAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorInsertExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-PI35P2
Plasmid#92419PurposeFluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.DepositorInsertMCOLN1 (Mcoln1 Mouse)
TagseGFPExpressionMammalianMutationSynthetic gene, codon optimized for human.PromoterCMVAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCri System
Plasmid Kit#1000000058PurposePlasmids used for cytoplasmic and periplasmic/extracellular heterologous protein expression in E. coli, Bacillus subtilis, and Pichia pastoris.DepositorApplicationGene Expression and LabelingVector TypeBacterial Expression, Yeast ExpressionAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarG
Plasmid#31221DepositorInsertCD4-tdGFP (CD4 Human, Fly, Aequorea victoria)
TagsCD4 and EGFP/GFPExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …Available SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom
Plasmid#25851DepositorAvailable SinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCut plasmid toolkit
Plasmid Kit#1000000118PurposePlasmids for integrating into well-characterized chromosomal loci to program gene expression in S. cerevisiae.DepositorApplicationGenome EditingVector TypeYeast ExpressionEditing TypeCRISPRAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPR219.05
Plasmid#78554PurposePlasmid expressing UirR, response regulator and PcsiR1-regulated GFP outputDepositorInsertuirR
UseSynthetic BiologyPromoterJ23115Available SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterUser-definedAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
YM138
Bacterial Strain#61953PurposeC43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.DepositorBacterial ResistanceNoneAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AdenoBuilder toolkit
Plasmid Kit#1000000176PurposePlasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypeAdenoviral, Mammalian ExpressionCloning TypeGibsonAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ubi:Zebrabow
Plasmid#118219PurposeFor ubiquitous expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG2
Plasmid#99576PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotypeDepositorTypeEmpty backboneExpressionMammalianPromoterhCMV IE1 gene promoterAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone
Pooled Library#75314PurposeHuman sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1_D73C
Plasmid#39328DepositorInsertMSP1E3D1_D73C (APOA1 synthetic gene)
Tags6xHisExpressionBacterialMutationcys mutant of MSP1E3D1PromoterT7Available SinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA Q75L
Plasmid#19719DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralExpressionMammalianMutationGTPase deficient & constitutively active Gl…Available SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only