167,895 results

1. Flag-HA-USP39

   Plasmid

   #22581

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP39 (USP39 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

2. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-SNAP-PI(3,4,5)P3 Probe

   Plasmid

   #211514

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BTK1-PH
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

   Plasmid

   #226516

   Purpose

   Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   TOMM20-SNAP-tag2-T2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-MAP2K1

   Plasmid

   #23406

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBintT

   Plasmid

   #48134

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HA-hIFITM1

   Plasmid

   #58399

   Purpose

   Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

   Depositor

   Howard Hang, Jacob Yount

   Article

   Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

   Insert

   IFITM1 (IFITM1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EGFR

   Plasmid

   #116731

   Purpose

   Lentiviral expression of EGFR

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. non-targeting control gRNA (BRDN0001148862)

   Plasmid

   #80263

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2/v5-DEST hGlut4

    Plasmid

    #18087

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut4 (SLC2A4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-hDAT

    Plasmid

    #32810

    Depositor

    Susan Amara

    Insert

    DAT (SLC6A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. HBx

    Plasmid

    #65463

    Purpose

    expresses HBx in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al Hepatology. 2004 Jun;39(6):1683-93.

    Insert

    HBV X protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_V5-NEUROD1

    Plasmid

    #215031

    Purpose

    Mammalian expression of human NEUROD1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    neuronal differentiation 1 (NEUROD1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLG009 (DRT type 1)

    Plasmid

    #157887

    Purpose

    Expresses the DRT type 1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 1 (RT-nitrilase)
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

    Plasmid

    #173452

    Purpose

    Contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

    Insert

    mCherry-sfGFP-ADH1ter
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. WDR59 in pRK5

    Plasmid

    #154214

    Purpose

    Untagged WDR59 cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    WDR59 (WDR59 Human)
    

    Tags

    NONE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CD80

    Plasmid

    #223603

    Purpose

    Lentiviral expression of human CD80 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid

    #113865

    Purpose

    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald-Clathrin-15

    Plasmid

    #54040

    Purpose

    Localization: Clathrin Vesicles, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

    Insert

    Clathrin (CLTB Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. Klotho (secreted)

    Plasmid

    #17713

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEBTet_NADP-Snifit-mito

    Plasmid

    #113919

    Purpose

    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3 deltaN89 Beta-catenin

    Plasmid

    #19288

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-89

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. paavCAG-JxON-pre-mGRASP-mCerulean

    Plasmid

    #51903

    Purpose

    JxOn-pre

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Insert

    presynaptic mGRASP
    

    Use

    AAV and Cre/Lox

    Promoter

    CAG

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid

    #220611

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGEX6p1-HH1713

    Plasmid

    #124994

    Purpose

    E. coli expression of recombinant H. hepaticus antigen HH_1713

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH_1713
    

    Expression

    Bacterial

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDGM3B

    Plasmid

    #110809

    Purpose

    contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

    Depositor

    David Russell

    Article

    Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

    Insert

    AAV3B cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-PH-TAPP1

    Plasmid

    #161985

    Purpose

    Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

    Depositor

    David Bryant, Dario Alessi

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PH-TAPP1 (PLEKHA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWZL Blast myc

    Plasmid

    #10674

    Depositor

    William Hahn

    Article

    Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

    Insert

    c-myc (MYC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV_SS-HA-V5-LaccID-CD4 TM_pDisplay

    Plasmid

    #234046

    Purpose

    expresses LaccID on the mammalian cell surface

    Depositor

    Alice Ting

    Article

    Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

    Insert

    LaccID-CD4TM
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAGEN-dPspCas13b-NES-mCherry

    Plasmid

    #233609

    Purpose

    Expression of catalytically-inactive PspCas13b in mammalian cells

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    dPspCas13b-NES-mCherry
    

    Use

    CRISPR

    Tags

    3xFlag and mCherry

    Mutation

    H133A/H1058A

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHAGE-CMV-hIL7-IRES-ZsGreen-W

    Plasmid

    #26532

    Depositor

    David Baltimore

    Insert

    IL7 (IL7 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRK5_mCherry_P2AT2A_GFP-nb

    Plasmid

    #238232

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. vHB8

    Plasmid

    #193454

    Purpose

    Expression of GFP-Cas9-NLS and gRNA in A. castellanii

    Depositor

    Chantal Abergel

    Article

    Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

    Insert

    GFP-Cas9-NLS
    

    Use

    CRISPR

    Tags

    GFP

    Promoter

    GAPDH

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HDM-Hgpm2

    Plasmid

    #204152

    Purpose

    Lentivirus helper plasmid coding for Gag/Pol

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    HIV-1 gag/pol
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

    Plasmid

    #156445

    Purpose

    For transient expression of mouse RAD21 (SCC1) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse RAD21 (Rad21 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMMP-TVA800

    Plasmid

    #15778

    Depositor

    Edward Callaway

    Article

    Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

    Insert

    TV A800
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGT-S1

    Plasmid

    #122582

    Purpose

    MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAGGS-synPCB2.1

    Plasmid

    #139478

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge