20,460 results

1. pCMV CEPIA4mt

   Plasmid

   #58220

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA4mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V E334D M339L F395W D436E

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pnsgRNA

   Plasmid

   #172717

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   J23119-gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-Rab21-T31N (DN)

   Plasmid

   #83423

   Purpose

   Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residue 31 from T to N (T31N: ACG -> AAC)

   Promoter

   CMV

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAX APP C99

   Plasmid

   #30146

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   APP C99 (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C-terminal from BACE site of APP

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

   Plasmid

   #29725

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHelper_ShCAST

   Plasmid

   #127922

   Purpose

   Expresses ShCAST and a crRNA targeting PSP1

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_SMARCB1_WT

   Plasmid

   #81791

   Purpose

   Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMARCB1 (SMARCB1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCrlM

   Plasmid

   #122444

   Purpose

   Expresses myc tagged mouse reelin ORF in mammalian cells

   Depositor

   Tom Curran

   Article

   D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

   Insert

   reelin (Reln Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET22b - PA

    Plasmid

    #84863

    Purpose

    bacterial expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Tags

    pelB signal sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZS165 CRISPEY HH-HDV NotI

    Plasmid

    #114451

    Purpose

    Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Type

    Empty backbone

    Use

    CRISPR; Cen/ars

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScGAL7p

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmTurquoise2-H2A

    Plasmid

    #36207

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PL-SIN-EOS-S(4+)-EGFP

    Plasmid

    #21317

    Purpose

    Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EOS-S(4+) promoter region

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23-MYC

    Plasmid

    #86461

    Purpose

    A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MYC

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ABE8.17-m

    Plasmid

    #136298

    Purpose

    expresses ABE8.17-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.17-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLIK-IDH2-FLAG

    Plasmid

    #66806

    Purpose

    Tet-inducible expression of IDH2 in mammalian cells

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH2 (IDH2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEos4b-N1

    Plasmid

    #54814

    Purpose

    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4b

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDsRed Monomer C1-YAP2

    Plasmid

    #19057

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Red-FPP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

    Plasmid

    #84467

    Purpose

    Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRAM and TRE

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cas9-GFP_sg_mTfeb

    Plasmid

    #79006

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    sgTfeb (Tfeb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAVS1-Puro cTnT FUCCI

    Plasmid

    #136935

    Purpose

    donor plasmid for cardiac-specific FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Troponin T promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cTnT

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162386

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR1A-Huwe1

    Plasmid

    #37431

    Depositor

    Jean Cook

    Insert

    Huwe1 (HUWE1 Human)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GST-Ub4

    Plasmid

    #171421

    Purpose

    For the purifcation of tetra linear ubiquitin

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    Ubiquitin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. P2-ECFP-pA (Construct 6)

    Plasmid

    #55198

    Purpose

    Synthetic promoter responsive to gRNA2

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal Ade MLP

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Pyronic/pcDNA3.1(-)

    Plasmid

    #51308

    Purpose

    Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

    Insert

    Pyronic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPROK AdoHcyase

    Plasmid

    #14683

    Depositor

    Michael Hershfield

    Article

    Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

    Insert

    adenosyl-homocysteine hydrolase (AHCY Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pCMV-myc3-HDM2

    Plasmid

    #20935

    Depositor

    Yue Xiong

    Article

    Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

    Insert

    Mdm2 p53 binding protein homolog (MDM2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDHt/sk-PE

    Plasmid

    #92126

    Purpose

    Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei

    Depositor

    Zhihua Zhou

    Article

    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

    Insert

    Cas9-eGFP
    

    Use

    CRISPR; Trichoderma reesei expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ppdc

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pARF1(Q71L)-CFP

    Plasmid

    #11385

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 71 to Leucine.

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUA

    Plasmid

    #58372

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Han et al Neuron. 2014 Feb 5;81(3):544-60. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pH1R-P2A-mCherry-N1

    Plasmid

    #84330

    Purpose

    Co-expresses (untagged) Histamine-1 receptor and mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H1R-P2A-mCherry (HRH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. YFP NLS Beta-Actin

    Plasmid

    #60613

    Purpose

    Encodes YFP and NLS tagged beta-actin

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hIRE1a wt

    Plasmid

    #20744

    Depositor

    Fumihiko Urano

    Article

    Lipson et al PLoS ONE. 2008 . 3(2):e1648.

    Insert

    IRE1 (ERN1 Human)
    

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX6P1-hcGAS(157-522)

    Plasmid

    #108676

    Purpose

    For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

    Insert

    cGAS amino acid 157-522 (CGAS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acid 157-522

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTP2620

    Plasmid

    #113311

    Purpose

    human Rad50 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Rad50 (RAD50 Human)
    

    Use

    Tags

    HA, 6xhis

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FUGW-pHRed

    Plasmid

    #65742

    Purpose

    Lentiviral vector for pHRed expression in mammalian cells using the hUbiC promoter

    Depositor

    Mathew Tantama

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    pHRed
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLVX-M-Flag-BAP1-C91A

    Plasmid

    #125841

    Purpose

    express mutant BAP1-C91A

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    C91A

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Flag-HA-OTUB1

    Plasmid

    #22551

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Double UP mNeon to mScarlet

    Plasmid

    #125134

    Purpose

    Natively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.

    Depositor

    Erik Dent

    Article

    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removed Not1 site in mScarlet

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. MyrPalm-CKAR

    Plasmid

    #14862

    Depositor

    Alexandra Newton

    Article

    Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

    Insert

    C kinase activity reporter
    

    Use

    Tags

    CFP, Myr/Palm, and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMVTNT-T7-KPNA5

    Plasmid

    #26681

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 5 (KPNA5 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLentiPGK Hygro DEST ERKKTRmCerulean3

    Plasmid

    #90229

    Purpose

    Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDONR223-TYK2

    Plasmid

    #23908

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TYK2 (TYK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAVS1-PC-GCaMP6f

    Plasmid

    #73503

    Purpose

    GCaMP6f knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGWB540

    Plasmid

    #74874

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSBtet-RB

    Plasmid

    #60506

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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