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175,644 results

Showing: 1001 - 1050 of 175644 results

  4. OPRM1-Tango

    Plasmid
    #66464
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    FLAG and tTA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66464-plasmid-map-sequence-id-157903

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  5. TACR1-Tango

    Plasmid
    #66514
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    TACR1 (TACR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pGL3 basic EGFP

    Plasmid
    #128053
    Purpose
    EGFP reporter vector with restriction sites to clone in a promoter of interest, derived from pGL3 basic
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128053-plasmid-map-sequence-id-255087

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  11. pLV-Hygro_hCOL4A3

    Plasmid
    #198098
    Purpose
    lentiviral expression of hCOL4A3 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A3 (COL4A3 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    198098-plasmid-map-sequence-id-467424

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  21. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available Since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  28. B-GRAM-W

    Plasmid
    #211707
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211707-plasmid-map-sequence-id-419219

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  32. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid
    #105648
    Purpose
    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks
    Depositor
    Sandra Rieger
    Insert
    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    Expression
    Mammalian
    Available Since
    Feb. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    105648-plasmid-map-sequence-id-202869

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  33. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid
    #123235
    Purpose
    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123235-plasmid-map-sequence-id-378779

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  37. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid
    #217319
    Purpose
    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.
    Depositor
    Noboru Mizushima
    Article
    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:
    Insert
    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    Use
    Retroviral
    Tags
    HaloTag and SNAP
    Promoter
    CMV
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    217319-plasmid-map-sequence-id-436845

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  39. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

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  41. PM-RA

    Plasmid
    #211705
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    PM-targetted RA (Lck Synthetic)
    Tags
    RA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211705-plasmid-map-sequence-id-419227

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  45. pMini-CMV-NLS-R-IscB-VR4-NLS (D60A, H243E, H269N, R270Q)_T2A_mCherry_U6-ωRNA

    Plasmid
    #246431
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. ssRNase enhancing mutations (H243E, H269N, R270Q) included.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB-VR4 (H243E, H269N, R270Q) with RuvC dead mutations (D60A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available Since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    246431-plasmid-map-sequence-id-496263

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  48. pJRA107

    Plasmid
    #209340
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209340-plasmid-map-sequence-id-420461

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Showing: 1001 - 1050 of 175644 results