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We narrowed to 25,498 results for: crispr

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Showing: 401 - 420 of 25498 results

  1. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Publication
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86708-plasmid-map-sequence-id-317051

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  6. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Publication
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44719-plasmid-map-sequence-id-472945

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  9. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61357-plasmid-map-sequence-id-358013

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  14. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6-BbsI-BbsI-PspCas13b DR-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    103854-plasmid-map-sequence-id-350782

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  20. pMCS-rybozyme-IRES-CAS9

    Plasmid
    #64668
    Purpose
    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.
    Depositor
    Wataru Fujii
    Publication
    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CAS9
    HDV ribozyme
    Use
    CRISPR
    Tags
    Flag, Internal ribosome entry site, and guide RNA…
    Expression
    Mammalian
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64668-plasmid-map-sequence-id-108759

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Showing: 401 - 420 of 25498 results