We narrowed to 23,588 results for: CHI

1. pGEX-4T-1-RIPK3

   Plasmid

   #78827

   Purpose

   To purify GST-tagged recombinant RIPK3 protein

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-AtC60αβC20NtL

   Plasmid

   #160876

   Purpose

   Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, cpn20
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcL: T7, chaperones: T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HiFi dCas9 KRAB

   Plasmid

   #188500

   Purpose

   Expresses FLAG tagged HiFi dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/R691A/H840A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE2-TetOminiCMV-Sox2

   Plasmid

   #136612

   Purpose

   Dox-inducible lentiviral vector expressing mouse Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLL3.7-K122-TEAD-p-H2B-mCherry

   Plasmid

   #139648

   Purpose

   Reporter assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   IRES-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX_970_puro_DEST_V5_TLOC1 (399aa)

   Plasmid

   #45288

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   TLOC1 (SEC62 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

7. Nicastrin-CD4d3+4-bio

   Plasmid

   #73106

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Nicastrin (NCSTN Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVXT-mWnt3

   Plasmid

   #121934

   Purpose

   doxycycline-inducible mouse Wnt3

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Wnt3 (Wnt3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. L1-4A

   Plasmid

   #13268

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pMIG II SIN-SV40-mCherry-SHP2

    Plasmid

    #180800

    Purpose

    Expression of mouse SHP2 fused to N-terminal mCherry

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP2 (Ptpn11 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

    Plasmid

    #213118

    Purpose

    Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    PKAsub-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4136458

    Plasmid

    #149531

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 13, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pFA6a-kanMX6-Purg1-3HA

    Plasmid

    #39351

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA and kanMX6 module - reverse orientation

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. HA-FOXO3a WT delta CT

    Plasmid

    #1796

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CT (aa468-673)

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pJH3628

    Plasmid

    #191445

    Purpose

    Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Chrimson
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRU5-inmCherry

    Plasmid

    #60238

    Purpose

    HTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intron

    Depositor

    Dmitriy Mazurov

    Article

    Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

    Insert

    inmCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EYFP-Clathrin

    Plasmid

    #20921

    Depositor

    Xiaowei Zhuang

    Article

    Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

    Insert

    Clathrin, light polypeptide (Lca) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

18. GlycophorinE-CD4d3+4-bio

    Plasmid

    #73095

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinE with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin E (GYPE Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCK004 ubi:mCerulean_SV40 (#302)

    Plasmid

    #195978

    Purpose

    Expression construct

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubi:mCerulean
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3XFlag DVL2 (∂361-736)

    Plasmid

    #24806

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of aa 361-736

    Promoter

    Available since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

21. HA-FOXO3a WT DBM (H212R)

    Plasmid

    #8352

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H212R

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pZmUBQ:AVRa1

    Plasmid

    #132356

    Purpose

    Expression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    AVRa1 ∆SP with stop codon
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-C1-hArfaptin2

    Plasmid

    #206405

    Purpose

    Mammalian expression of hArfaptin2

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

    Insert

    Arfaptin2 (ARFIP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGTag-P2A-QFGal4-SV40pA

    Plasmid

    #155127

    Purpose

    Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    P2A-QFGal4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRCP

    Plasmid

    #195862

    Purpose

    Encodes chromogenic protein (CP). CP inducible with rhamnose.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Insert

    Chromogenic Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. IFI16-(192-393)-HinA (pcDNA3-FLAG)

    Plasmid

    #35062

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Contains HinA domain: aa 192-393

    Promoter

    CMV

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pCDNAIIIB cdc42 WT

    Plasmid

    #61635

    Purpose

    mammalian expression of cdc42 wt

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    cdc42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

30. IFI16-(515-710)-HinB (pcDNA3-FLAG)

    Plasmid

    #35063

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(515-710)-HinB (IFI16 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains HinB domain: aa 515-710

    Promoter

    CMV

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

31. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBabe-puro-RIPK3

    Plasmid

    #78829

    Purpose

    retroviral expression of RIPK3

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

33. mCerulean3-H2B-6

    Plasmid

    #55421

    Purpose

    Localization: Nucleus/Histones, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pET-NtLAtC60αβC20

    Plasmid

    #160873

    Purpose

    Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: pBAD, chaperones: T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAH243

    Plasmid

    #121147

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HM3C NaVAe1_p pET24b

    Plasmid

    #127854

    Purpose

    Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1p
    

    Use

    Tags

    3C Cleavage site, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 5HT6-YFP-Inpp5e(D477N)

    Plasmid

    #96809

    Purpose

    phosphatase-dead control for 5HT6-YFP-Inpp5e, CAAX-null

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-Inpp5e(D477N)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGK:TRL-EFS:iRFP670

    Plasmid

    #68475

    Purpose

    Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    iRFP670
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and pGK

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. p40phox-PX (1-144)

    Plasmid

    #119123

    Purpose

    Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    p40phox-PX (1-144)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSNA

    Plasmid

    #135192

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

    Plasmid

    #167877

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid

    #160881

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. p2CT-His-MBP-Lbu_C2c2_R1079A

    Plasmid

    #83494

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1079A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1079A

    Promoter

    T7

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pUE1-TSP-ORF68 R443A

    Plasmid

    #162652

    Purpose

    Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acid R443 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pJH2523

    Plasmid

    #191358

    Purpose

    Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP3
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-25

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGBW-m4136464

    Plasmid

    #149534

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. pNIC28-BSA4-G-L11

    Plasmid

    #127830

    Purpose

    Production of recombinant human ribosomal protein L11

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPL11 (RPL11 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pzyxin-emiRFP703

    Plasmid

    #136568

    Purpose

    Zyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    zyxin-emiRFP703
    

    Use

    Tags

    emiRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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