We narrowed to 23,588 results for: CHI

1. PGRMC2-CD4d3+4-bio

   Plasmid

   #73112

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   PGRMC2 (PGRMC2 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRJPaph-gusA

   Plasmid

   #67036

   Purpose

   Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   gusA
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-bArrestin2-TevC-P2A-TdTomato

   Plasmid

   #89869

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (please see depositor comments …

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RCAS (A) Wnt3a (CT#169)

   Plasmid

   #13976

   Depositor

   Cliff Tabin

   Article

   Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

   Insert

   Wnt3a (Wnt3a Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCDNAIIIB rhoA WT

   Plasmid

   #61631

   Purpose

   mammalian expression of rhoA

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   rhoA (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMC_mNG2(11)_BSDminus_F0

   Plasmid

   #184162

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKG209

   Plasmid

   #45109

   Depositor

   Iain Cheeseman

   Article

   Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

   Insert

   CENP-T (aa1-374) Laci-NLS (CENPT Human)
   

   Use

   Tags

   GFP, Laci-NLS, s peptide, and tev

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCph8

   Plasmid

   #22869

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

   Insert

   pCph8
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   First 517 amino acids of Cph1 and the last 229 am…

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NAX_CAGGS_CAS9_m4_VPR_EBFP2

   Plasmid

   #167881

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

    Plasmid

    #83483

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. BAS288 C terminal mKO2 natR

    Plasmid

    #74083

    Purpose

    S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    mKO2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-HsSENP3(307-574)

    Plasmid

    #71481

    Purpose

    Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-306 (catalytic domain only)

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pSin-NE1m

    Plasmid

    #172883

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    J. Julius Zhu

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Insert

    NE SENSOR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJH4330

    Plasmid

    #191700

    Purpose

    Punc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GtACR2
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-17

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3xFNLDD/pMXs-puro

    Plasmid

    #49536

    Purpose

    Expresses 3xFNLDD in mammalian cells

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

    Insert

    3xFNLDD
    

    Use

    Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJH4329

    Plasmid

    #191679

    Purpose

    Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GtACR2
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PlmdCas12e KRAB

    Plasmid

    #188506

    Purpose

    Expresses FLAG tagged PlmdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-KRAB-NLS

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMALp2x-SCO5461

    Plasmid

    #42507

    Depositor

    Koji Okamoto

    Article

    Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

    Insert

    guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
    

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 22, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pRG/III-hs-MBP-A2aTr316-H10

    Plasmid

    #121683

    Purpose

    Bacterial expression of adenosine A2a receptor fusion protein

    Depositor

    Richard Henderson, Reinhard Grisshammer

    Article

    Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

    Insert

    adenosine A2a receptor (ADORA2A Human)
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCold I-Hero9

    Plasmid

    #187927

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero9

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero9
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. C8orf55-CD4d3+4-bio

    Plasmid

    #73126

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding C8orf55 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    C8orf55 (THEM6 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NAX_CAGGS_CAS9_m4_VPR_Puro

    Plasmid

    #167887

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOPS0253

    Plasmid

    #115505

    Purpose

    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMIR-Dsred-IRES-His Keap

    Plasmid

    #62457

    Purpose

    expresses his tagged human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    HisTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCFL-mPML-3KRmut-iB

    Plasmid

    #140287

    Purpose

    Expression of mouse PML KR mutant

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    PML (Pml Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS-TRE-mTagBFP2-WPRE

    Plasmid

    #193332

    Purpose

    Expresses mTagBFP2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A XhoI site in the coding sequence is silenced

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG Fzd7 GFP

    Plasmid

    #66106

    Purpose

    expression of chicken Fzd7 GFP

    Depositor

    Felicitas Proels, Martin Scaal

    Article

    et al Development. 2015 Feb 15;142(4):665-71.

    Insert

    Fzd7 (FZD7 Chicken)
    

    Use

    Chicken expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

29. mEos2-Alpha-Actinin-19

    Plasmid

    #57346

    Purpose

    Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pBabe_puro_DEST_Flag_SLC2A2

    Plasmid

    #45267

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

31. GADD45 P-3m-luc

    Plasmid

    #8358

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter P-3m (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    P-3m

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pMJA181

    Plasmid

    #128539

    Purpose

    Expresses anti-Prunus dulcis scFv Ab+BirA tag

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6 plus BirA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. YFP Gamma 3

    Plasmid

    #36103

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 3 (Gng3 Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pAYC

    Plasmid

    #62657

    Purpose

    Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pETM41-EcLonA

    Plasmid

    #38330

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    Lon protease fragment (lon Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Polyphosphate binding fragment of Lon protease. …

    Promoter

    T7

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

36. mCerulean3-EB1-C-18

    Plasmid

    #55415

    Purpose

    Localization: MT End Binding Protein, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pPSP6

    Plasmid

    #48150

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBABE-Hygro HoxB9

    Plasmid

    #27022

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    HoxB9 (HOXB9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

39. BAS292 C terminal SF-GFP kanR

    Plasmid

    #74087

    Purpose

    S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    SF-GFP recoded for S.pombe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

40. pBstSK+IRES-V5-IPpoI

    Plasmid

    #206321

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag- IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PX458_CIC_iso1_2

    Plasmid

    #135744

    Purpose

    Encodes gRNA for 3' target of human CIC_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    CIC_iso1 gRNA (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PX458_CIC_iso1_1

    Plasmid

    #135743

    Purpose

    Encodes gRNA for 3' target of human CIC_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    CIC_iso1 gRNA (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

    Plasmid

    #107317

    Purpose

    Expresses SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9
    

    Use

    CRISPR

    Tags

    3xHA and 2xNLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCEP4-myc-H2-Ld

    Plasmid

    #135519

    Purpose

    Mammalian expression of myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGBW-m4046940

    Plasmid

    #149261

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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46. pDUAL_2_T5

    Plasmid

    #155175

    Purpose

    see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. mRFP-dmBACCS2

    Plasmid

    #67630

    Purpose

    Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    mRFP-dmBACCS2
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Drosophila actin gene 5C gene promoter

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. ama1CD4d3+4-bio

    Plasmid

    #36145

    Purpose

    EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ama1 (PF3D7_1133400 Plasmodium falciparum)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pPN217

    Plasmid

    #91676

    Purpose

    Express sgRNA targeting human TLE1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TLE1 (TLE1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMH-HT_ygiF-CHAD

    Plasmid

    #127793

    Purpose

    Expresses the CHAD domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF-CHAD (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 201-433)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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