161,271 results

1. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

   Plasmid

   #194685

   Purpose

   CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry_a_tubulin_IRES_puro2

   Plasmid

   #21043

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV Marina-T2A-nls-mCherry

   Plasmid

   #74216

   Purpose

   genetically-encoded fluorescent voltage indicator

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   fluorescent protein voltage sensor
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RCASBP-AP (A) (CC#37)

   Plasmid

   #15160

   Depositor

   Connie Cepko

   Article

   Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

   Insert

   PLAP (ALPP Human)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag Rab12 wild type

   Plasmid

   #86787

   Purpose

   mammalian expression of Rab12

   Depositor

   Peter McPherson

   Article

   Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

   Insert

   Rab12 (RAB12 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLIK-Hygro

   Plasmid

   #25737

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-21a-PE2-His

   Plasmid

   #201973

   Purpose

   Express His-tagged PE2 in bacteria

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   PE2-HIS
   

   Use

   Tags

   His and c-myc NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPDK3876(TRV-RNA2-pPEBV-MCS)

    Plasmid

    #149275

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSFLPe

    Plasmid

    #31130

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEX-MCS UBC promoter

    Plasmid

    #128045

    Purpose

    Lentiviral vector with UBC promoter

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Syn61Δ3(ev5) ΔrecA (ev1)

    Bacterial Strain

    #189857

    Purpose

    Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

    Depositor

    George Church

    Article

    Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Synthetic

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3 Flag p38 delta

    Plasmid

    #20785

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    pDCNA3 Flag p38 delta (Mapk13 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mu-GM-CSF-pCIneo

    Plasmid

    #163518

    Purpose

    Expresses murine GM-CSF in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine GM-CSF
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4

    Plasmid

    #163026

    Purpose

    FLiCRE TF for HEK293T expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    CD4-MKII-hLOV1-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

    Plasmid

    #192184

    Purpose

    Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 6 heavy chain (HH06)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

    Plasmid

    #192183

    Purpose

    Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 heavy chain (HH02)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC18T-mini-Tn7T-Gm-ecfp

    Plasmid

    #65030

    Purpose

    GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSFFV-SpyTag-sfCherry2(11)-TagBFP

    Plasmid

    #117485

    Purpose

    Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyTag002-GG-sfCherry2(11)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSCM001

    Plasmid

    #124605

    Purpose

    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

    Depositor

    David Summers

    Article

    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

    Insert

    mCherry-pHluorin
    

    Use

    Tags

    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

    Expression

    Bacterial

    Mutation

    Promoter

    pDAB

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194687

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-Flag Rac2

    Plasmid

    #12192

    Depositor

    Gary Johnson

    Article

    Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

    Insert

    Rac2 (RAC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRE132

    Plasmid

    #118486

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT/TO-eGFP-53BP1

    Plasmid

    #60813

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid

    #233036

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

    Depositor

    Jonathan Ploski

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pIRE YIC

    Plasmid

    #18784

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    H-ferritin iron response element
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. phOCT4-Luc

    Plasmid

    #17221

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    Upstream region of human OCT4 (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSYFP2-SCFP3A

    Plasmid

    #22905

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

    Insert

    SYFP2-SCFP3A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMEH373 dTomato 3x high affinity MS2 loops

    Plasmid

    #168221

    Purpose

    Mammalian expression of dTomato with 3 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    dTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV_EF1aUBA3_IRES_Puro

    Plasmid

    #235660

    Purpose

    human UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)

    Depositor

    Lorenz Studer

    Article

    Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

    Insert

    UBA3 (UBA3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV5-ActRIIB

    Plasmid

    #13501

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    Activin receptor IIB (Acvr2b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pEGFP-C1-TFIIB

    Plasmid

    #26675

    Depositor

    Sui Huang

    Article

    Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

    Insert

    TFIIB (GTF2B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only