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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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161,271 results

Showing: 1001 - 1040 of 161271 results

  10. p5-71

    Plasmid
    #118085
    Purpose
    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    Depositor
    Bryan Dickinson
    Article
    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d
    Insert
    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118085-plasmid-map-sequence-id-227374

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  13. pAAV-Ef1a-Coff/Fon-GCaMP6F (AAV8)

    Viral Prep
    #137124-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6F (#137124). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6F plasmid DNA. EF1a-driven, Flp-dependent expression of GCaMP6f (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    None
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. Flag-miniTurbo

    Plasmid
    #124647
    Purpose
    Expresses Flag-miniTurbo fusion for proximity labeling
    Depositor
    Ken-Ichi Takemaru, FENG-QIAN Li
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124647-plasmid-map-sequence-id-242444

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  15. pMitoTimer

    Plasmid
    #52659
    Purpose
    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo
    Depositor
    Zhen Yan
    Article
    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1
    Insert
    DsRed1-E5
    Use
    Dsred
    Tags
    human cytochrome c oxidase subunit VIII mitochond…
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    52659-plasmid-map-sequence-id-430007

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  21. pFUGW-FIRE-pHLy

    Plasmid
    #170774
    Purpose
    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    hUbC
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170774-plasmid-map-sequence-id-339008

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  22. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV9)

    Viral Prep
    #100833-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Tags
    None
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. pSL1-GFP

    Plasmid
    #180420
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.
    Depositor
    Nancy Moran
    Article
    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180420-plasmid-map-sequence-id-357217

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  26. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  36. pAAV-CAG-GFP (AAV2)

    Viral Prep
    #37825-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    Oct. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1001 - 1040 of 161271 results