167,825 results

1. FUGW-Golgi-TurboID

   Plasmid

   #200964

   Purpose

   3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Golgi-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPT41

   Plasmid

   #85524

   Purpose

   Titration sponge for TetR

   Depositor

   Johan Paulsson

   Article

   Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Promoter

   PLtet

   Available Since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

   Plasmid

   #100054

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   channelrhodopsin-2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CAG

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 Myc2- Hs TTP WT

   Plasmid

   #107008

   Purpose

   Expresses epitope-tagged wild-type human TTP

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   tristetraprolin (ZFP36 Human)
   

   Tags

   Double Myc peptide epitope tag

   Expression

   Mammalian

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Casp2-Flag

   Plasmid

   #11811

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 (CASP2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57 (Gent)

   Plasmid

   #54338

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTipi2.1-AZU_SP

   Plasmid

   #229144

   Purpose

   Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

   Depositor

   Institute for Protein Innovation (IPI)

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 9, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLV_Zika_NS5_Flag

   Plasmid

   #79639

   Purpose

   Third generation Self-inactivating lentivector expressing NS5 from Zika virus

   Depositor

   Vaithi Arumugaswami

   Insert

   NS5
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Tollip

   Plasmid

   #178050

   Purpose

   GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_RAD51_Rad51

    Plasmid

    #110031

    Purpose

    Protein expression and purification of RAD51_Rad51

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RAD51_Rad51 (RAD51 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTipi2.1

    Plasmid

    #225877

    Purpose

    Allows for expression of recombinant antibodies

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pB_tetO7_sfGFP

    Plasmid

    #196616

    Purpose

    Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    Superfolder GFP
    

    Expression

    Yeast

    Promoter

    tetO7 promoter

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. UbG76V-eGFP-V5His_20aa

    Plasmid

    #23970

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_20aa
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Available Since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. aMHC-mCherry-Rex-Blasticidin

    Plasmid

    #21228

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    mCherry

    Available Since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFPc3 K-RAS 4B

    Plasmid

    #236452

    Purpose

    transient overexpression of K-RAS 4B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    K-RAS 4B (KRAS Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Brachyury-eGFP

    Plasmid

    #21063

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-SRGAP2A-EGFP

    Plasmid

    #168501

    Purpose

    Expression of full length SRGAP2 with a C-termonal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A (SRGAP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG1b

    Plasmid

    #80612

    Purpose

    pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_DNASE2_WT

    Plasmid

    #81815

    Purpose

    Gateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    DNASE2 (DNASE2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

    Plasmid

    #239948

    Purpose

    Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    Fc2-MFSD6(L3)
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAc-C Med12His

    Plasmid

    #49240

    Purpose

    expresses human Med12 with His tag in insect cells

    Depositor

    Dylan Taatjes

    Article

    Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

    Insert

    Med12 (MED12 Human)
    

    Tags

    His

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHLUK

    Plasmid

    #64181

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFYF1548 EMX1

    Plasmid

    #47508

    Purpose

    human gRNA expression vector targeting EMX1

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EMX1 (EMX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBT120b

    Plasmid

    #122604

    Purpose

    BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    gIII, luxAB, guide RNA
    

    Use

    Synthetic Biology

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-HA-GBF1

    Plasmid

    #206415

    Purpose

    Mammalian expression of hGBF1

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pKD-SepRS-EFSep

    Plasmid

    #34623

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Inserts

    SepRS (MMP0688 Methanocaldococcuc maripaludis)
    EF-Sep (tufB E. coli)
    

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Ptac and Ptac (from SepRS- operon like)

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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29. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid

    #137730

    Purpose

    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-4T-1 3xHA-MEK1-DD

    Plasmid

    #47576

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MEK1-DD (Map2k1 Mouse)
    

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser218 to Asp, Ser221 to Asp

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pU6_rd6_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232433

    Purpose

    pegRNA with optimized 3' modifications to correct the rd6 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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32. pBIG2ab

    Plasmid

    #80616

    Purpose

    pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMB-SaCas9

    Plasmid

    #105998

    Purpose

    SaCas9 expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Insert

    Cas9 nuclease
    

    Use

    CRISPR and Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    short EF1alpha

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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34. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230019

    Purpose

    AAV vector expressing anti-mCherry PAGER(Gi) in neurons

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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35. CMV-hEAAT1

    Plasmid

    #32813

    Depositor

    Susan Amara

    Insert

    EAAT1 (SLC1A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RXFP1-Tango

    Plasmid

    #66492

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP1 (RXFP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pLV_Zika_NS3_Flag

    Plasmid

    #79635

    Purpose

    Third generation Self-inactivating lentivector expressing NS3 from Zika virus

    Depositor

    Vaithi Arumugaswami

    Insert

    NS3
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EF1a_SOX10_P2A_Hygro_Barcode

    Plasmid

    #120479

    Purpose

    Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTRE3G-GSX2

    Plasmid

    #96964

    Purpose

    Dox-inducible expression of Gsx2 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GSX2 (Gsx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TroponinT-GCaMP5-Zeo

    Plasmid

    #46027

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    GCaMP5-2A-Zeo
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TNNT2

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge