173,387 results
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Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneTagsmyc, HisExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCG-Hc∆18-AA
Plasmid#86559PurposePlasmid encodes blinded measles virus H glycoproteinDepositorInsertMeasles virus H glycoprotein
ExpressionMammalianMutationY463A, R515AAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2629
Plasmid#142966PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-HA-BirA-GSGS-Traf6
Plasmid#232586PurposeExpress BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
luxB:cp157Venus+luxA/pRSETB Duet
Plasmid#197583PurposeBRET lux luciferase with cp157Venus for bacterial expressionDepositorInsertLuxA LuxBcp157Venus
UseE.coli expressionTagsHis X 6ExpressionBacterialPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
ExpressionBacterialAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga15
Plasmid#196060PurposeEncodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
NW11
Plasmid#133443PurposeCovalently circularized nanodisc NW11 for studying membrane proteins and viral entry.DepositorInsertNW11
ExpressionBacterialAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Antibody#201857-rAbPurposeAnti-c-Myc chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rat IgG2a Fc.DepositorRecommended ApplicationsWestern BlotReactivityHumanSource SpeciesRatIsotypeIgG2aTrial SizeAvailable to purchaseAvailable SinceJuly 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pNL[NlucP/NFAT-RE/Hygro] Vector
Plasmid#236929PurposeExpress NFAT response element in luciferase reporter assayDepositorHas ServiceDNAInsertNFAT
UseLuciferaseTagsNanoLuc (R)MutationNonePromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH2B-miRFP703
Plasmid#80001PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP703DepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51084-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES
Plasmid#69909PurposeExpresses a GFP circular RNA containing the sense EMCV IRES in mammalian cellsDepositorAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-LMNB1
Plasmid#207770PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB
Plasmid#228869PurposePE7 connected to MLH1-SB via 2A sequenceDepositorInsertPE7-2A-MLH1-SB
UseCRISPRAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP14696: pAAV-AiE0779m_6xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4696)
Plasmid#214859PurposeExpression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1 MSNs), stronger than 3xCore version CN3044)DepositorInsertSYFP2
UseAAVAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDD162 (Peft-3::Cas9 + Empty sgRNA)
Plasmid#47549PurposeCas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.DepositorInsertsCas9
Empty sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available SinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6-10 (fl)
Plasmid#137820Purposeexpression of CD44 proteins in mammalian cellsDepositorAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-ARHGAP26
Plasmid#69467PurposeExpresses ARHGAP26DepositorAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB14a-GFP
Plasmid#126117PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23119Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ
Plasmid#109380PurposeRhamnose inducible LacZ expression. Used to test burden response in E. coliDepositorInsertpRHAM-LacZ
ExpressionBacterialAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti
Plasmid#138315PurposeLenti expression of mTgBFP2DepositorInsertmTAGBFP2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3R1
Plasmid#179136PurposeExpression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
ExpressionMammalianAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEmax-mRNA
Plasmid#204472Purposeplasmid for PEmax mRNA IVTDepositorInsertPrime editor
UseCRISPRTagsnoExpressionMammalianPromoterT7Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
h-alpha-ENaC
Plasmid#83430Purposefull length human alpha ENaC with Flag tagDepositorAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 deltaN89 Beta-catenin
Plasmid#19288DepositorInsertbeta-catenin (CTNNB1 Human)
TagsFlagExpressionMammalianMutationN-terminal truncation, deleted aa 1-89Available SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA
Plasmid#109314PurposePlasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-MyosinIIC-N-18
Plasmid#55108PurposeLocalization: Cytoskeleton, Excitation: 587, Emission: 610DepositorInsertMyosinIIC
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA
Plasmid#107050PurposeAll-in-one vectors expressing both SaCas9 and YFP sgRNADepositorInsertSaCas9
UseAAV and CRISPRExpressionMammalianAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6f.WPRE.SV40
Plasmid#100837PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro
Plasmid#154263PurposeDox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.DepositorInsert3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
Tags3xFLAGExpressionMammalianPromoterTRE3GAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNAHygro(+)LAMP2B
Plasmid#86029PurposeExpresses LAMP2B in Mammalian cellsDepositorAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-M337V
Plasmid#133759PurposeLentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged methionine 337 to valinePromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
ExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEV4
Plasmid#110539PurposeThe plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR223-LMP1
Plasmid#195805PurposeGateway Entry Clone of Epstein-Barr virus LMP1DepositorInsertLMP1 (LMP-1 Epstein-Barr virus)
UseGateway entry vectorAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6347 MSCV-CMV-Flag-HA-Brd4-444-722
Plasmid#31353DepositorAvailable SinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only