167,318 results

1. pAAV-U6-hSyn::mCherry.3xFLAG-WPRE

   Plasmid

   #120392

   Purpose

   pAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

   Type

   Empty backbone

   Use

   AAV

   Tags

   mCherry.3XFLAG

   Promoter

   U6

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYC-N

   Plasmid

   #207113

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2 YFP

   Plasmid

   #84910

   Purpose

   expression of YFP in mammalian cells

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   YFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. 460 p3E-2A-mCherrypA

   Plasmid

   #195970

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCherry
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-eNme2-C-ABE8e

   Plasmid

   #185667

   Purpose

   Expresses eNme2-C-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-C
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-TFE3

   Plasmid

   #38120

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFE3 (TFE3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. mGreenLantern

   Plasmid

   #197928

   Purpose

   Monomer expression vector

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

   Insert

   mGreenLantern
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMTL5

   Plasmid

   #127967

   Purpose

   DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV9)

    Viral Prep

    #192552-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Guoping Feng

    Promoter

    CAG

    Tags

    tdTomato

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pLV-mTurquoise2-Caspase-1-mNeonGreen

    Plasmid

    #166784

    Purpose

    FRET analysis (pro caspase-1 cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. scFv-GCN4-sfGFP-TET1CD

    Plasmid

    #184439

    Purpose

    TET1 catalytic domain fused with sfGFP and scFv against GCN4

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    scFv-GCN4-sfGFP-TET1CD
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. negMaLionG

    Plasmid

    #113907

    Purpose

    negative control for Green ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    negMaLionG
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_STAT1_WT

    Plasmid

    #82234

    Purpose

    Gateway Donor vector containing STAT1, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-dAPEX2

    Plasmid

    #117173

    Purpose

    Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    dAPEX2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNF02-mLemon

    Plasmid

    #219399

    Purpose

    Constitutive expression of mLemon in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mLemon
    

    Expression

    Bacterial

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA6-ARID1A

    Plasmid

    #39311

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    ARID1A (ARID1A Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87828

    Purpose

    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCINeo-myc-Itch/2859

    Plasmid

    #11427

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Itch (Itch Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-IRAlus-Nicn1

    Plasmid

    #92345

    Purpose

    Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

    Depositor

    Ling-Ling Chen, Gordon Carmichael

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. LV-GFP

    Plasmid

    #25999

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-GFP (H2BC21 A. victoria, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. mCherry-Ezrin-N-14

    Plasmid

    #55043

    Purpose

    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Ezrin (EZR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. AAV-cfGFP

    Plasmid

    #200773

    Purpose

    Expresses cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    cysteine-free GFP
    

    Use

    AAV

    Promoter

    Synapsin

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-CAG-dLight1.1 (AAV5)

    Viral Prep

    #111067-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

26. pGH125_dCas9-Blast

    Plasmid

    #85417

    Purpose

    lentiviral expression vector for dCas9 with Blasticidin selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    dCas9 and Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSF11-wmiRFP680-T2A-HO1

    Plasmid

    #197244

    Purpose

    Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP680-T2A-HO1
    

    Expression

    Worm

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. Golden GATEway Cloning Kit

    Plasmid Kit

    #1000000054

    Purpose

    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way

    Depositor

    Joachim Wittbrodt

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

29. mCherry-Parkin

    Plasmid

    #23956

    Purpose

    Mammalian expression of human Park2 fused to mCherry

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Park2 (PRKN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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30. ZsGreen1-N1

    Plasmid

    #54702

    Purpose

    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    ZsGreen1

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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