167,318 results

1. Frt-hspB3

   Plasmid

   #63094

   Purpose

   Expression of HSPB3 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb3 (HSPB3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA7CR_2.0

   Plasmid

   #73950

   Purpose

   pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Inserts

   cas9
   lambda beta
   dam
   recX
   

   Expression

   Bacterial

   Promoter

   pAra and pTet

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSyn.iGluSnFR3.v857.PDGFR (AAV1)

   Viral Prep

   #178329-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.PDGFR (#178329). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.PDGFR plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

6. pDONR221_SLC25A25

   Plasmid

   #131992

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A25 (SLC25A25 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMaCTag-P19

   Plasmid

   #124789

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry-mNeonGreen
   

   Use

   Pcr template

   Tags

   mCherry-mNeonGreen

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TMPRSS2

   Plasmid

   #53887

   Purpose

   Expression of TMPRSS2

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Has Service

   Cloning Grade DNA

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Tet-off Full length Tau (0N4R, P301L/V337M)

   Plasmid

   #188573

   Purpose

   Dox-repressible expression of the 0N4R Tau isoform

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   0N4R Tau (MAPT Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TUBB5-Halo

    Plasmid

    #64691

    Purpose

    Expresses beta-tubulin labeled with Halo-tag

    Depositor

    Yasushi Okada

    Article

    Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

    Insert

    TUBB
    

    Tags

    Halo-tag

    Expression

    Mammalian

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDB364

    Plasmid

    #110275

    Purpose

    N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    10-His, TEV cleavage peptide, and mature DsbC (i.…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1683

    Plasmid

    #142826

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TWIST1 (TWIST1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

    Plasmid

    #184632

    Purpose

    Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad67 (Gad1 Mouse)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ilux pGEX(-)

    Plasmid

    #107879

    Purpose

    Bacterial expression of ilux for bioluminescence imaging

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

    Insert

    ilux
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIVT-Cas9-TMV

    Plasmid

    #238016

    Purpose

    for DNA-free gene knockout editing in rice and wheat or other plants

    Depositor

    Caixia Gao

    Article

    Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

    Insert

    SpCas9
    

    Expression

    Plant

    Promoter

    T7

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIVT-Cas9-DEN2

    Plasmid

    #238017

    Purpose

    for DNA-free gene knockout editing in rice and wheat or other plants

    Depositor

    Caixia Gao

    Article

    Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

    Insert

    SpCas9
    

    Expression

    Plant

    Promoter

    T7

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRL_SFFVp-EGFP

    Plasmid

    #244971

    Purpose

    Express EGFP under SFFV promoter. Xho I-SFFVp-AgeI-EGFP.

    Depositor

    Xinnan Wang

    Article

    Kwak et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    SFFVp-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDK9-HiBiT Vector

    Plasmid

    #238588

    Purpose

    Express CDK9-HiBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CDK9 (CDK9 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

    Plasmid

    #187079

    Purpose

    Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL
    

    Expression

    Mammalian

    Mutation

    dHaloTag7 = HaloTag7-D106A

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pPBpuro-ERK-KTR-mCherry

    Plasmid

    #214829

    Purpose

    Encoding a fluorescent reporter for ERK activity

    Depositor

    Michiyuki Matsuda

    Article

    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

    Insert

    ERK KTR-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

    Plasmid

    #236368

    Purpose

    Overexpression of HyperdLbCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdLbCas12a
    

    Use

    CRISPR and Lentiviral

    Mutation

    D156R, D235R, E292R, D350R

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. hVPS4B-HaloTag Vector

    Plasmid

    #236975

    Purpose

    Express hVPS4B-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    hVPS4B (VPS4B Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. hVPS4A-HaloTag Vector

    Plasmid

    #236977

    Purpose

    Express hVPS4A-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    hVPS4A (VPS4A Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pcDNA3 HA Cyclin F

    Plasmid

    #236466

    Purpose

    transient overexpression of Cyclin F in mammalian cells

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

    Insert

    Cyclin F (CCNF Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3 2xSTREP Cyclin F(LP35/36AA)

    Plasmid

    #236467

    Purpose

    transient overexpression of Cyclin F in mammalian cells

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

    Insert

    Cyclin F (CCNF Human)
    

    Tags

    2xSTREP

    Expression

    Mammalian

    Mutation

    (LP35/36AA) first two amino acids of the F-box do…

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLenti-0.5Syn-D2GFP-WPRE

    Plasmid

    #233060

    Purpose

    To express D2GFP from a 0.5 bp synapsin promoter.

    Depositor

    Jonathan Ploski

    Insert

    D2GFP
    

    Use

    Lentiviral

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

    Plasmid

    #231721

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    POLR2A (POLR2A Human)
    

    Tags

    Flag-GST-3C

    Expression

    Insect

    Mutation

    RPB1:1586-1971

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pNX24-RFP-MS2SL

    Plasmid

    #227819

    Purpose

    Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    3xHA-RFP-24xMS2SL
    Neo resistance
    

    Expression

    Mammalian

    Promoter

    CMV and SV40

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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