We narrowed to 16,166 results for: GRN

1. pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #200502

   Purpose

   Lentiviral vector expressing gRNA targeting human AAVS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   AAVS1 (AAVS1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

   Plasmid

   #200503

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

   Plasmid

   #200505

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Promoter

   EFS, No promoter, and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

   Plasmid

   #49772

   Purpose

   Level 2 construct

   Depositor

   Sophien Kamoun

   Article

   Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

   Insert

   sgRNA_PDS2-Cas9-sgRNA_PDS1
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

   Plasmid

   #133347

   Purpose

   expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_cpaA and sgRNA_blaA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

   Plasmid

   #133348

   Purpose

   expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA and sgRNA_cpaA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

   Plasmid

   #197028

   Purpose

   AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enOsCas12f1
   

   Use

   AAV

   Mutation

   D52R / T132R

   Promoter

   MHCK7, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Expression

   Bacterial

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. px458_2A_GFP_sgRNA_LTN1

    Plasmid

    #127125

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA LTN1 (LTN1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCgsgRNA_crtYf

    Plasmid

    #153338

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    crRNA targeting crtYf
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSPneoHHgRNAH

    Plasmid

    #63557

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6>sgRNA(F+E)

    Plasmid

    #59986

    Purpose

    Empty vector for sgRNA cloning (F+E modified backbone)

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    Ciinte.U6

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. p102_gRNA_Sa_mClover

    Plasmid

    #213778

    Purpose

    Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sa gRNA
    

    Use

    CRISPR and Lentiviral

    Promoter

    mU6

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p103_gRNA_Sp_mClover

    Plasmid

    #213779

    Purpose

    Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sp gRNA
    

    Use

    CRISPR and Lentiviral

    Promoter

    mU6

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Expression

    Bacterial

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPEgRNA

    Plasmid

    #172716

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    gRNA
    

    Expression

    Bacterial

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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