167,120 results
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pAV-U6+27-Tornado-RhoBAST-MS2
Plasmid#196342PurposeOverexpression of circular RhoBAST-MS2DepositorInsertTornado-RhoBAST-MS2
UseTagsExpressionMammalianMutationPromoterU6+27Available SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 HA-Nprl2
Plasmid#99709PurposeMammalian expression of HA-Nprl2DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/6xHis mCherry F30 2xdBroccoli
Plasmid#169233PurposeConstitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.DepositorInsert6xHis mCherry F30-2xdBroccoli
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable SinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-GT
Plasmid#37814DepositorInsertPromotorless gfp reporter gene
UseTagsExpressionMutationPromoterAvailable SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lyn-R-GECO1
Plasmid#120410PurposeExpresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.DepositorInsertLyn-R-GECO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-Giantin
Plasmid#186575PurposeExpression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)DepositorAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEM-GECO1
Plasmid#32442PurposeMammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEM-GECO1
UseTagsExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/DEST/hTfR-HA
Plasmid#69610Purposehuman transferrin receptor with an HA tag fused at the C-terminusDepositorInserthuman transferrin receptor (TFRC Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[21-881]-C-int
Plasmid#199202PurposeGateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expressionDepositorInsertsplit-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-BLM
Plasmid#110299PurposeMammalian expression of a EGFP-tagged full length Bloom's syndrome helicaseDepositorInsertBLM (Bloom's Syndrome) helicase (BLM Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
UseTagsExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23A
Plasmid#91138PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-mtkeima
Plasmid#210338PurposeOverexpression of mtkeimaDepositorInsertmtkeima
UseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
UseTagsN-terminal His6 tagExpressionBacterialMutationPromoterAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse lipid metabolism knockout library
Pooled Library#206248PurposeThe lipid metabolism library contains guide RNAs against 295 genes targeting mouse genes encoding enzymes that directly impact lipid metabolism.DepositorUseCRISPR and LentiviralAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-M2
Plasmid#44170DepositorInsertInfluenza A virus M2 matrix gene
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Puro-iNgn3
Plasmid#75337Purposedoxycycline inducible Ngn3 over-expressionDepositorAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-cTNT-Cre
Plasmid#209785PurposeExpresses Cre by the specific cTNT promoterDepositorInsertCre
UseAAVTagsExpressionMutationPromotercTNTAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro PPAR delta
Plasmid#8891DepositorAvailable SinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
UseTagshuman H2BExpressionInsectMutationPromoter10xUASAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorAvailable SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF1A
Plasmid#172206PurposeExpresses KIF1A tagged with eGFP in mammalian cellsDepositorAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-dead Tet1CD-P2A-BFP
Plasmid#108246PurposeLentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFPDepositorInsertdCas9-dead Tet1CD-HA-P2A-BFP
UseLentiviralTagsHAExpressionMammalianMutationdCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…PromoterUbcAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRE YIC
Plasmid#18784DepositorInsertH-ferritin iron response element
UseTagsExpressionMammalianMutationPromoterAvailable SinceAug. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-HaloTag (w876-1)
Plasmid#29644DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseEntry vectorTagsHaloTagExpressionMutationPromoterAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12D
Plasmid#81651PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC476 - pAAV c-fos eYFP
Plasmid#47907PurposeAn AAV vector that expresses eYFP under the c-fos promoter.DepositorInsertenhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMutationPromoterc-fosAvailable SinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
hROSA26 CRISPR-pX330
Plasmid#105927PurposehROSA26 targeting CRISPR plasmidDepositorInserthROSA26 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.ArcLightD.WPRE.SV40
Plasmid#100037PurposeAAV expression of ArcLightD driven by hSynap promoterDepositorInsertArcLightD
UseAAVTagsExpressionMammalianMutationPromoterhSynapAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-NGN2
Plasmid#197090PurposePiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiationDepositorAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHimar6
Plasmid#137081Purposebacterial Himar1 mini-transposon donor plasmidDepositorInsertHimar1 mini-transposon with chloramphenicol resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVone-AAV2-CMV-eGFP
Plasmid#230930PurposepAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.DepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-mBaoJin-oPRE
Plasmid#218683PurposeAAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInsertmBaoJin
UseAAVTagsExpressionMammalianMutationPromoterHuman synapsin promoterAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP
Plasmid#154139PurposeExpresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.DepositorInsertdCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
UseTags3x FLAG and tagBFPExpressionMammalianMutationPromoterCMVAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
NT-CRISPR plasmid collection
Plasmid Kit#1000000189PurposeThe NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.DepositorAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV2)
Viral Prep#100054-AAV2PurposeReady-to-use AAV2 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic EKAR (Cerulean-Venus)
Plasmid#18679DepositorInsertERK Activity Reporter
UseTagsmonomeric Cerulean and monomeric VenusExpressionMammalianMutationPromoterAvailable SinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
Flag-Gsdmd-CT
Plasmid#80953PurposeExpresses Gsdmd-CT in mammalian cellsDepositorAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only
