163,819 results

1. pLX304-ERBB2

   Plasmid

   #175846

   Purpose

   Expresses ERBB2

   Depositor

   Evan Braunstein

   Article

   Braunstein et al Leukemia. 2016 Nov;30(11):2242-2245. doi

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti_sgRNA(MS2)_zsGreen1

   Plasmid

   #138463

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   zsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLIK-Hygro

   Plasmid

   #25737

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5-myc-Miro1 E208K/E328K

   Plasmid

   #47894

   Purpose

   Expresses myc tagged Miro1 E208K/E328K mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro1 E208K/E328K (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   E208K/E328K, abolishes calcium binding

   Promoter

   CMV

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. G13-CASE

   Plasmid

   #168127

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at F125/D126 within GNA13

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. DD-DamV133A-tdTomato-LMNB1

   Plasmid

   #159604

   Purpose

   Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   DD-DamV133A-tdTomato-LMNB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dam V133A mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FAT FAK biosensor

   Plasmid

   #78303

   Purpose

   FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

   Insert

   FAT FAK biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-FLEX-P301L Tau

   Plasmid

   #176267

   Purpose

   This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

   Depositor

   Tsuneya Ikezu

   Article

   You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

   Has Service

   AAV1

   Insert

   Flex-P301L tau (MAPT )
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458-3xHA-en1FnCas9

   Plasmid

   #201947

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en1FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1369R on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. epB_UbC_CymRV5-nls-GFP_DEx2

    Plasmid

    #119906

    Purpose

    ePiggyBac mammalian expression plasmid for CymR-GFP fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    CymRV5-nls-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pScPPX1

    Plasmid

    #233713

    Purpose

    Bacterial expression of the yeast PPX1 gene

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    ScPpx1 (PPX1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCAT077

    Plasmid

    #180511

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1-puro U6 sgRNA BfuAI stuffer

    Plasmid

    #50920

    Purpose

    U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-FLAG-Claspin

    Plasmid

    #12659

    Depositor

    Michele Pagano

    Article

    Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

    Plasmid

    #84480

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLF3FShC

    Plasmid

    #153083

    Purpose

    RMCE integration vector with self excising cassette

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-VHL-pRc/CMV

    Plasmid

    #19999

    Depositor

    William Kaelin

    Article

    Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7-LacO-LgBiT

    Plasmid

    #216779

    Purpose

    Expresses LgBiT in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    LgBiT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-TRIF-CFP

    Plasmid

    #13644

    Depositor

    Doug Golenbock

    Insert

    TRIF (TICAM1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Halo-Sec31

    Plasmid

    #166895

    Purpose

    mammalian expression of Sec31 tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    Sec31
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS513 EF1alpha-cre

    Plasmid

    #11918

    Purpose

    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYPQ150

    Plasmid

    #69301

    Purpose

    Gateway entry vector with pcoCas9 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9 (plant codon-optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Flag-FOXI1

    Plasmid

    #153126

    Purpose

    Expression of Flag tagged human FOXI1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXI1 (FOXI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCnCre

    Plasmid

    #232907

    Purpose

    Electroporation vector for expression of Cre recombinase in Cuprividus necator

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    Cre recombinase (cre Synthetic)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ptac-cymO

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pI3_EGFP

    Plasmid

    #239335

    Purpose

    Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmScarlet3_I3

    Plasmid

    #239342

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. fabp10a:ER-CreER; cryaa:mCerulean

    Plasmid

    #230077

    Purpose

    Expression of ERCreER in zebrafish hepatocytes.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    ERT2-Cre-ERT2
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available since

    May 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pAAV hSyn INTRON mCherry-Jph3 WPRE

    Plasmid

    #236237

    Purpose

    AAV expression of human Junctophilin3 N-terminally tagged with mCherry

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    Junctophilin 3 (JPH3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMY.PRC2

    Plasmid

    #234582

    Purpose

    Expression of ProtA-EZH2, SUZ12, EED and RBBP4

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pColdI-DDX3_helicase_core

    Plasmid

    #233618

    Purpose

    Expression of His-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core WT (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-S5E6-dTom-nlsdTom (AAV1)

    Viral Prep

    #135641-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-S5E6-dTom-nlsdTom (#135641). In addition to the viral particles, you will also receive purified pAAV-S5E6-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato in VIP cortical interneurons under the control of the E6 regulatory element. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Promoter

    Tags

    dTomato

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

33. R2pH-LAMPx3ALFA

    Plasmid

    #225136

    Purpose

    R2pH-LAMP1-labelled lysosomes with 3xLFA

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRDB_161

    Plasmid

    #216091

    Purpose

    Cas9 [Sp] CRISPRi targeting CD81, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD81 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EC67892

    Plasmid

    #209499

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TFORF3460

    Plasmid

    #144936

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PSMB1 (PSMB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

    Plasmid

    #177849

    Purpose

    Bacterial Expression of PNR

    Depositor

    Karsten Melcher

    Article

    Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

    Insert

    photoreceptor cell-specific nuclear receptor (NR2E3 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

    Plasmid

    #125430

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCR1319

    Plasmid

    #111116

    Purpose

    Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T gRNA (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge