We narrowed to 10,946 results for: UTY

1. JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)

   Plasmid

   #233542

   Purpose

   A CMV promoter driving expression of a murine dominant negative myc tagged TCF4.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF4 (TCF4 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   lacks aa 1-56

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

   Plasmid

   #232128

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA2 (GATA2 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

   Plasmid

   #232123

   Purpose

   A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

   Plasmid

   #232129

   Purpose

   A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   MAFB (MAFB Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

   Plasmid

   #232131

   Purpose

   A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF2 (FOXF2 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

   Plasmid

   #232130

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA-DAB1

   Plasmid

   #162691

   Purpose

   Expresses DabAB1 inorganic carbon transport complex

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   DabAB1 inorganic carbon transport complex
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEAST_J23101-vdh

   Plasmid

   #128134

   Purpose

   The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   vdh
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAB051e

   Plasmid

   #79195

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PExt-10cons lambda op-62 gIII, luxAB; Ptet lambda cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PExt-10cons lambda op-62; Ptet

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAB078d7

    Plasmid

    #79205

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (off-target) luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (off-target); Ppro1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAB085e

    Plasmid

    #79212

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ptet

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBbi 41BBL-CD86

    Plasmid

    #183244

    Purpose

    Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    CD86, 41BBL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdCASclos

    Plasmid

    #73640

    Purpose

    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    dCas9
    sgRNA to spo0A
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    Pj23119 and ptb

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCCM’

    Plasmid

    #162709

    Purpose

    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    p15A origin replaced with high-copy colE1 origin

    Promoter

    PLtet0-1 promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCB’

    Plasmid

    #162703

    Purpose

    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5513T>G (tetR E37A); 7758G>T (second tet op…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSH624-ssr

    Plasmid

    #199241

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    single-stranded DNA recombineering
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptrc

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RSV-Flag-Brd2

    Plasmid

    #86614

    Purpose

    Flag tagged mouse Brd2 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd2 (Brd2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-LUC2CP/ARE

    Plasmid

    #62857

    Purpose

    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    destabilizing sequences (DS) added to the C termi…

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RSV-Flag-Brd4

    Plasmid

    #86616

    Purpose

    Flag tagged mouse Brd4 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd4 (Brd4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RSV-Flag-Brd3

    Plasmid

    #86615

    Purpose

    Flag tagged mouse Brd3 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd3 (Brd3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMIA4.721-Pansio

    Plasmid

    #214441

    Purpose

    BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVAX1-BMP2

    Plasmid

    #137909

    Purpose

    Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

    Depositor

    Georg Feichtinger

    Article

    Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

    Insert

    BMP2 (BMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183606

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Pgk1

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC

    Plasmid

    #135465

    Purpose

    Membrane-anchored TF used for yeast directed evolution of TEV protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
    

    Use

    Tags

    BFP, flag

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

    Plasmid

    #233549

    Purpose

    A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMIA4.721-Keppel

    Plasmid

    #214444

    Purpose

    BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMIA4.721-Olônne

    Plasmid

    #214443

    Purpose

    BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJBEI-6411

    Plasmid

    #47050

    Purpose

    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDFDuet-MKK6-EE

    Plasmid

    #82718

    Purpose

    Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

31. pCS2+ Zf GFP-vclb

    Plasmid

    #185411

    Purpose

    GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin b (vclb Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

    Plasmid

    #183609

    Purpose

    Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    Neo
    mKate2-3xNLS
    

    Use

    Mouse Targeting

    Tags

    3xNLS

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCS2+ Zf GFP-vcla

    Plasmid

    #185410

    Purpose

    GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin a (vcla Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFE-Selo-FI-prk

    Plasmid

    #162698

    Purpose

    Expresses S. elongatus Form IB rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    S. elongatus Form IB rubisco and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFE-Rrub-FII-prk

    Plasmid

    #162695

    Purpose

    Expresses R. rubrum Form II rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Inserts

    Rubrum Form II rubisco
    S. elongatus PRK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter and under same promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-AKAP79-Dronpa

    Plasmid

    #182332

    Purpose

    AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    AKAP79-Dronpa (AKAP5 Human)
    

    Use

    Tags

    Full-length AKAP79

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCCM

    Plasmid

    #162708

    Purpose

    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG668 lifeAct-pFAST

    Plasmid

    #172866

    Purpose

    Expresses LifeAct-pFAST in mammalian cells (actin labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    LifeAct-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-H2B-μDpTT

    Plasmid

    #182328

    Purpose

    Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    H2B-μDronpa-TagRFP-T (H2BC3 Human)
    

    Use

    Tags

    Full-length human histone H2B

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSNV2 CHER NLS cop1at(335-675)

    Plasmid

    #44972

    Depositor

    Thanos Halazonetis

    Article

    Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

    Insert

    mCherry-NLS-COP1 (COP1 Mustard Weed)
    

    Use

    Tags

    NLS and mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-334

    Promoter

    pCMV IE

    Available since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCS2+ Zf cdh1-deltaCyot-GFP

    Plasmid

    #185412

    Purpose

    GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    cdh1 (cdh1 Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain deleted

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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42. pAG671 mito-pFAST

    Plasmid

    #172867

    Purpose

    Expresses mitochondrial targeting domain-pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    mito-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAG657 H2B-pFAST

    Plasmid

    #172862

    Purpose

    Expresses pFAST fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    H2B-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pENTR1A DHFR.cHSF1

    Plasmid

    #134737

    Purpose

    Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    DHFR

    Expression

    Mutation

    Deletion of amino acids 186-202

    Promoter

    None

    Available since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAG665 MAP4-pFAST

    Plasmid

    #172865

    Purpose

    Expresses MAP4-pFAST in mammalian cells (microtubule labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    MAP4-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

    Plasmid

    #233546

    Purpose

    A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    FOXF1 (FOXF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

    Plasmid

    #232132

    Purpose

    A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    FOXF1 (FOXF1 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET21a(+)_co_L-HicDH

    Plasmid

    #185453

    Purpose

    CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

    Depositor

    Wolfgang Kroutil

    Article

    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

    Insert

    L-HicDH from Lactobacillus confusus DSM 20196
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. attB53-Pac-tetO-mCherry-attB53

    Plasmid

    #183612

    Purpose

    RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    tetO-mCherry
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    tetO

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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