165,545 results

1. pRN12

   Plasmid

   #84456

   Purpose

   shuttle plasmid for sarA P1-mAmetrine expression

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   mAmetrine
   

   Use

   Shuttle vector e.coli-s.aureus, expression of sgf…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-ConVERGD-eGFP-WPRE

   Plasmid

   #218749

   Purpose

   Provides AND intersectional (Cre+Flp) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCGN-HCF-1 fl

   Plasmid

   #53309

   Purpose

   plasmid contains human HCFC1 full length gene with HA and c-myc tags

   Depositor

   Winship Herr

   Article

   Wilson et al Cell. 1993 Jul 16;74(1):115-25.

   Insert

   HCFC1 (HCFC1 Human)
   

   Use

   Tags

   HA and c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pv6_CBX7_Chromo_TAF3_Phd

   Plasmid

   #179400

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo - TAF3_Phd
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-Hb9-GFP

   Plasmid

   #37080

   Purpose

   3rd generation lentiviral transfer plasmid for Hb9 GFP

   Depositor

   Fred Gage

   Article

   Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

   Insert

   Hb9 GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Hb9

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_hEF1a_rtTA3

   Plasmid

   #61472

   Purpose

   3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Insert

   rtTA3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hEF1a

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-EGFR

   Plasmid

   #23935

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-EF1a-CD90.2-Rluc_miR

   Plasmid

   #163360

   Purpose

   Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD90.2 (Thy1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p15A-PB

   Plasmid

   #133886

   Purpose

   piggyBac ITR's in low copy number p15A backbone plasmid

   Depositor

   Mario Capecchi

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEND-184_Cacnes_mCherry

    Plasmid

    #225610

    Purpose

    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AB65_pCAG.Cas9-D10A.rBGpA

    Plasmid

    #199256

    Purpose

    Expression construct encoding the SpCas9-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SpCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_ADAR2DD_E488Q_Ago2

    Plasmid

    #205599

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    hAgo2 (AGO2 Human)
    

    Use

    Tags

    ADAR2DD_E488Q (hyperactive mutant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_Pro-IL-1β

    Plasmid

    #214340

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1B (IL1B Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCpf1b-sp

    Plasmid

    #122188

    Purpose

    Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-mGCN2-FLAG-IP

    Plasmid

    #101794

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Gcn2 (Eif2ak4 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_XPR2_hrGFP

    Plasmid

    #84614

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Activin A (S2-2)

    Plasmid

    #184621

    Purpose

    for transfection into S2 cells and expression of Activin A protein

    Depositor

    Timothy Springer

    Article

    Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

    Insert

    INHBA (INHBA Human)
    

    Use

    Tags

    8x His - streptavidin binding peptide

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-FLEX-EGFP (AAV1)

    Viral Prep

    #59331-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEX-EGFP (#59331). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-EGFP plasmid DNA. CAG-driven, Cre-dependent expression of EGFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ian Wickersham

    Promoter

    CAG

    Tags

    EGFP

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

21. TFORF1287

    Plasmid

    #142778

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SALL2 (SALL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PGK EGFP

    Plasmid

    #216110

    Purpose

    PGK EGFP

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    PGK promoter
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-28c S2

    Plasmid

    #171920

    Purpose

    Express RNA-based (p)ppGpp sensor

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Insert

    RNA-based (p)ppGpp sensor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNH-TrxT

    Plasmid

    #26106

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis, TEV cleavage site, and TrxA

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLL3.7m-psMEK1

    Plasmid

    #89361

    Purpose

    A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psMEK1 (MAP2K1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

    Plasmid

    #124362

    Purpose

    Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-aptNFkB#5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RFP-PASS

    Plasmid

    #193971

    Purpose

    Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

    Depositor

    Guangwei Du

    Article

    Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

    Insert

    PA biosenor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCR1326

    Plasmid

    #111131

    Purpose

    Expresses UBE2T.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge