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Addgene

We narrowed to 2,450 results for: LRE

Showing: 2101 - 2150 of 2450 results
  1. pCSIIpuro-AREG-ScNeo

    Plasmid
    #209897
    Purpose
    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Insert
    AREG-ScNeo (AREG Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pCSIIpuro-EPGN-ScNeo

    Plasmid
    #209899
    Purpose
    To monitor the status of Epigen, the plasmid encodes a recombinant Epigen fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Insert
    EPGN-ScNeo (EPGN Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pCSIIpuro-EREG-ScNeo

    Plasmid
    #209896
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Insert
    EREG-ScNeo (EREG Human)
    Use
    Lentiviral
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Promoter
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pMXs-E55A-ATPIF1

    Plasmid
    #85404
    Purpose
    Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase
    Depositor
    Insert
    ATPIF1 (ATP5IF1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    E55A point mutation abrogating the ability of ATP…
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid
    #206114
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid
    #50834
    Purpose
    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.
    Depositor
    Insert
    hChR2 (H134R)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    EF1a
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

    Plasmid
    #171952
    Purpose
    All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3
    Insert
    serine and arginine rich splicing factor 3 (SRSF3 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized, truncated at amino acid 114 with…
    Promoter
    TRE
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pTRE-SOX17-P2A-Venus-rpEF1a-Zeo

    Plasmid
    #169174
    Purpose
    piggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175
    Depositor
    Insert
    SOX17-P2A-Venus (SOX17 Synthetic, Human)
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTREtight (tet-inducible promoter)
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid
    #176149
    Purpose
    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette
    Depositor
    Insert
    X-ray repair cross complementing 1 (XRCC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…
    Promoter
    CMV
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. Tom20-mChF-AIP(TD)

    Plasmid
    #61521
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutation
    Depositor
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Use
    Tags
    AIP with TD mutation (see comments), Tom20, and m…
    Expression
    Mammalian
    Mutation
    TD mutation (see comments)
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2101 - 2150 of 2450 results