We narrowed to 2,450 results for: LRE
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Plasmid#132716PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphinDepositorInsertGFP-shDyn (Pdyn Rat)
UseAAVTagsExpressionMutationPromoterSynapsin 1Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-AREG-ScNeo
Plasmid#209897PurposeTo monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertAREG-ScNeo (AREG Human)
UseLentiviralTagsmNeonGreen and mScarletExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-DNTGFBR2-HA
Plasmid#130888PurposeExpresses dominant negative mutant human TGFBR2DepositorInsertTGFBR2 (TGFBR2 Human)
UseTagsHA-tagExpressionMammalianMutationDominant Negative receptor lacking cytosolic cata…PromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-EPGN-ScNeo
Plasmid#209899PurposeTo monitor the status of Epigen, the plasmid encodes a recombinant Epigen fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertEPGN-ScNeo (EPGN Human)
UseLentiviralTagsmNeonGreen and mScarletExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-EREG-ScNeo
Plasmid#209896PurposeTo monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertEREG-ScNeo (EREG Human)
UseLentiviralTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)
Plasmid#115788PurposeExpresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.DepositorInsertLRP5 (LRP5 Human)
UseTags3xflagExpressionMammalianMutationcontains silent mutation that creates an internal…PromoterCMVAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-CD79B-YFP
Plasmid#187006PurposeExpression of human CD79B as YFP fusion proteinDepositorInsertCD79B (CD79B Human)
UseLentiviralTagsYFPExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNGVL4a-hCRTmE6mE7mL2
Plasmid#104153PurposeExpresses human calreticulin fused to MmuPV1 E6, MmuPV1 E7 and MmuPV1 L2 residues 11-210 in mammalian cellsDepositorUseTagsExpressionMammalianMutationresidues 11 to 200 of the late protein L2PromoterCMVAvailable sinceDec. 18, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFL-EGFP-3xFlag-HLTF
Plasmid#193055PurposeBaculovirus expression of human HLTF for recombinant protein productionDepositorInsertHLTF (HLTF Human)
UseTags3xFlagExpressionInsectMutationPromoterpolyhedronAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS-WT
Plasmid#44978DepositorInsertFUS (Full length) (FUS Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-GIGYF1
Plasmid#141188PurposeExpresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell lineDepositorInsertGIGYF1 (GIGYF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-STAT3mts
Plasmid#195577PurposeExpresses STAT3 with mitochondrial targeting sequenceDepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAAVTagsCox8 presequenceExpressionMammalianMutationPromoterCMV enhancer and promoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_huMcl-1.1
Plasmid#85530PurposeInducible expression of guide RNA (huMcl-1.1) with fluorescent GFP reporterDepositorInserthu Mcl-1.1 (MCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDK9 gRNA (BRDN0001162576)
Plasmid#77170Purpose3rd generation lentiviral gRNA plasmid targeting human CDK9DepositorInsertCDK9 (CDK9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIA3
Plasmid#109399PurposeExpresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSBDepositorTypeEmpty backboneUseCRISPRTagsmRuby2 and mtp53dnExpressionMammalianMutationPromoterEF1aAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
Plasmid#122085PurposeExpress M.EcoGII fused to CENPC with destabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
UseRetroviralTagsV5 tag on Nter of CENPCExpressionBacterial and MammalianMutationL341F, R930L (see comments below)PromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SBECN1W
Plasmid#107937PurposeAdeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neuronsDepositorInsertmouse Beclin-1 (BECN1) (Becn1 Mouse)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL WT
Plasmid#107159PurposeFor mammalian expression of Flag-hPKL WTDepositorInsertFlag-hPKL WT (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse Ezh2 Y637S-IRES-GFP
Plasmid#91887PurposeRetroviral expression of mouse Ezh2 with Y637SDepositorInsertEzh2 (Ezh2 Mouse)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationY637SPromoterMMLV gagAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-E55A-ATPIF1
Plasmid#85404PurposeRetroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthaseDepositorInsertATPIF1 (ATP5IF1 Human)
UseRetroviralTagsExpressionMammalianMutationE55A point mutation abrogating the ability of ATP…PromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE
Plasmid#139093PurposeExpresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdxCas9-NG-VPR
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…PromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse Ezh2 delta SETdomain-IRES-GFP
Plasmid#91885PurposeRetroviral expression of mouse Ezh2 with a SET domain deletionDepositorInsertEzh2 (Ezh2 Mouse)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationSET domain deletedPromoterMMLV gagAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP
Plasmid#91879PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2DepositorInsertgRNA targeting Ezh2 (Ezh2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDK9 gRNA (BRDN0001146478)
Plasmid#77169Purpose3rd generation lentiviral gRNA plasmid targeting human CDK9DepositorInsertCDK9 (CDK9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-STAT3
Plasmid#195575PurposeExpresses STAT3DepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterCMV enhancer and promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgHcn1#2/Cre
Plasmid#193218PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 geneDepositorInsertsgHcn1#2 (Hcn1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE
Plasmid#139097PurposeExpresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dxCas9-NG
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…PromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D122A pcDNA3
Plasmid#206120Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA E1658A (EIVA) pcDNA3
Plasmid#206114Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 EIVA (E1707A) pcDNA3
Plasmid#206125Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE1707APromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EIA (E320A) pcDNA3
Plasmid#206124Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320APromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP
Plasmid#91880PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2DepositorInsertgRNA targeting Ezh2 (Ezh2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
Plasmid#50834PurposeAn AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.DepositorInserthChR2 (H134R)
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationH134RPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSN25
Plasmid#100110Purposeexpresses a chimeric SOX2-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast
Plasmid#171952PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(721-1176aa)
Plasmid#139690PurposeExpresses N-teminal Flag tagged aa 721-1176 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1
Plasmid#206006PurposeExpression of SomArchon-mTagBFP2 and CoChR under Syn promoterDepositorInsertSomArchon-mTagBFP2-P2A-CoChR-Kv2.1
UseAAVTagsmTagBFP2ExpressionMammalianMutationPromoterhSynAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSico_U6-Pan-PGC1a sgRNA
Plasmid#165426PurposeExpression of gRNA against human Total PGC-1a variantsDepositorInsertgRNA against human Total PGC-1a variants (PPARGC1A Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE-SOX17-P2A-Venus-rpEF1a-Zeo
Plasmid#169174PurposepiggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175DepositorInsertSOX17-P2A-Venus (SOX17 Synthetic, Human)
UsePiggybac transposonTagsExpressionMammalianMutationPromoterpTREtight (tet-inducible promoter)Available sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS-DELTA-UNE-L
Plasmid#139691PurposeExpresses N-teminal Flag tagged aa 1- 1064 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-XRCC1-gRNA res-Neo
Plasmid#176149PurposeXRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassetteDepositorInsertX-ray repair cross complementing 1 (XRCC1 Human)
UseLentiviralTagsExpressionMammalianMutationDual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…PromoterCMVAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
Plasmid#51249PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 980,1006 mutated to AlaPromoterEF1aAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE
Plasmid#139092PurposeExpresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdxCas9 3.7-VPR
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…PromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-deltaExon2
Plasmid#105671PurposeMammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)DepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
Plasmid#51247PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSN33
Plasmid#100118Purposeexpresses a chimeric KLF4-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TD)
Plasmid#61521PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TD mutation (see comments), Tom20, and m…ExpressionMammalianMutationTD mutation (see comments)PromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
CDK10 gRNA (BRDN0001145808)
Plasmid#77578Purpose3rd generation lentiviral gRNA plasmid targeting human CDK10DepositorInsertCDK10 (CDK10 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK10 gRNA (BRDN0001149446)
Plasmid#77579Purpose3rd generation lentiviral gRNA plasmid targeting human CDK10DepositorInsertCDK10 (CDK10 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only