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pMM2-hPMCA2x/b

Plasmid

#47582

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2x/b (ATP2B2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hH1

Plasmid

#102841

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_hH1

Use

Pcr template for stagr inserts

Promoter

hH1

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH_blast_MCS_Nard_GFP_D50

Plasmid

#167339

Purpose

Expresses GFP fused to progerin (d50) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Progerin

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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NET29A.Hs pEGFP-N2 (1181)

Plasmid

#62040

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET29A (TMEM120A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Splice variant

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mCherry (JDW 1240)

Plasmid

#224499

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mCherry

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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opto-Cdc42

Plasmid

#174510

Purpose

Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Cdc42-mCherry

Tags

3xFLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDTnLAPP2A frame 0

Plasmid

#28226

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCI-OR2J2

Plasmid

#22321

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

OR2J2 (OR2J2 Human)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pMS29: pDonor(PmcCAST)

Plasmid

#168162

Purpose

PmcCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST right end (RE)
PmcCAST left end (LE)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Giantin

Plasmid

#186575

Purpose

Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

Giantin (GOLGB1 Human)

Tags

FRB-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-MCS

Plasmid

#122558

Purpose

Modified block 1, with deletion in E1

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Mutation

Adenovirus sequences 481-3530 replaced with multi…

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG

Plasmid

#124930

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

Plasmid

#113889

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U1-70K-fSNAPc

Plasmid

#106987

Purpose

open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

U1-70K (SNRNP70 Human)

Tags

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcU6_1 sgRNA

Plasmid

#92395

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.1 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.1

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(E17K)

Plasmid

#86563

Purpose

Expresses human full-length Akt1(E17K) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

E17K mutation in the PH domain

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNAKv10.1mVenus

Plasmid

#85705

Purpose

Kv10.1 fused mVenus

Depositor

Luis Pardo

Article

Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

Insert

KCNH1 (KCNH1 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTK50_GFP-NuMA-N

Plasmid

#46348

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA N-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

N-terminal fragment from 1-213

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut9

Plasmid

#18088

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut9 (SLC2A9 Human)

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag DLC2

Plasmid

#24269

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pOPS0750

Plasmid

#115511

Purpose

Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

gusA pOGG083, T-pharma pOGG003 assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Constitutive promoter pNeo pOGG001

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A10

Plasmid

#132275

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A10 (SLC39A10 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAM4941 (pCV0053)

Plasmid

#120083

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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ACE2(-202)-luc

Plasmid

#31112

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -202 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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3495 pcDNA3 Bid p15

Plasmid

#8780

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p15 (Bid Mouse)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
SPLICa:ER-MT (High Affinity)

Plasmid

#247995

Purpose

Expresses the SPLICa reporter for ER-Mitochondria contacts and Calcium transients at the contact

Depositor

Tito Calì

Article

Yamaguchi et al Anal Chem. 2025 Sep 16;97(36):19848-1986

Insert

SPLICa ER-MT (h)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-LivePARBackbone

Plasmid

#176526

Purpose

Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-MPP7

Plasmid

#23411

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MPP7 (MPP7 Human)

Use

Gateway Cloning

Mutation

2MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Vamp3-pHluorin

Plasmid

#190152

Purpose

Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp3 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-EGFP-SOS1

Plasmid

#178860

Purpose

Mammalian expression of SOS1 fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

SOS1 (SOS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p3s-ABE7.10

Plasmid

#113128

Purpose

expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE 7.10

Tags

HA epitope and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-ThymosinBeta4(KK18,19EE)

Plasmid

#96807

Purpose

non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)

Tags

YFP

Expression

Mammalian

Mutation

KK18,19EE prevents G-actin binding

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIP SECFP-Stx17TM

Plasmid

#86777

Purpose

Expresses transmembrane domains of syntaxin17 tagged with SECFP in mammalian cells

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

transmembrane domains of human Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

SECFP

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1E3D1

Plasmid

#199772

Purpose

Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

Insert

cMSP1E3D1

Tags

His6-Tag

Expression

Bacterial

Mutation

Extended cMSP1D1; external His-Tags flanking the …

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 8

Plasmid

#36106

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 8 (GNG8 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
YFP Gamma 1

Plasmid

#36101

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 1 (GNGT1 Bovine)

Tags

YFP

Expression

Mammalian

Promoter

cmv

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pCDH-EF1a-HygroR-T2A-TetOn3G

Plasmid

#179887

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

Hygromycin resistance

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-TMCC1

Plasmid

#120932

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
3xDBE_RE:NLuc

Plasmid

#178318

Purpose

FOXO NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

FOXO NLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTM-BPL

Plasmid

#173766

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Article

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

PDGF receptor TM domain, Biotin protein ligase

Tags

FLAG and Signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FKBP-INPP4BC842A

Plasmid

#116865

Purpose

Inactive recruitable PI(3,4)P2 4-phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

INPP4B (INPP4B Human)

Tags

FKBP12 and mCherry

Expression

Mammalian

Mutation

catalytic cysteine 842 changed to alanine

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS-H2B-citrine

Plasmid

#53752

Purpose

Contains fusion of human Histone H2B to citrine.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

citrine

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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