We narrowed to 5,933 results for: chia
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Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
ExpressionBacterialMutationNahR: I25V P72H V118A R248CAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
plYJP022-int7
Plasmid#202287PurposePlasmid containing integrase for LPattB7 siteDepositorInsertint7
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro
Plasmid#99636PurposeEmpty backbone, 3rd generation lentiviral vectorDepositorTypeEmpty backboneUseLentiviralTagsnoneExpressionMammalianPromoterSFFVpAvailable SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Nkx2-1/Puro
Plasmid#31271DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
ExpressionBacterialMutationAraC: L133F E165L E169G C280LAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCNA
Plasmid#135191PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-CreI.DepositorInsertI-CreI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBDK
Plasmid#135190PurposeIntegration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selectionDepositorInsertKanR
UseSynthetic BiologyExpressionBacterialPromotercatAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMON-HsNMT1
Plasmid#67475PurposeExpression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteriaDepositorInsertHsNMT1 (NMT1 Human)
ExpressionBacterialAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB2_AB
Plasmid#98534Purposeempty BB2 for transcription unit cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.474
Plasmid#108526PurposeLuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.DepositorInsertLuxR + PLuxB-YFP
ExpressionBacterialMutationVanR: T49I A111V P179SAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 100
Plasmid#52989PurposepLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfpDepositorInsertsOxyS sRNA
fhlA
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutation−309 to +96 bp relative to start codonPromoterpLlac0-1 and pLtetO-1Available SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJM.847
Plasmid#108524PurposePhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.DepositorInsertPhlFAM + PPhlF-YFP
ExpressionBacterialMutationPhlF: S50G S73I R95H A121V M137I N144D T155I R200CAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
SupD v2
Plasmid#198598PurposePlasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.DepositorInsertSupD
ExpressionBacterialAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJA179
Plasmid#128537PurposeExpresses anti-Prunus dulcis scFv AbDepositorInsertscFv Ab clone PD1F6
ExpressionYeastPromoterAOX1Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRXG
Plasmid#113643PurposeThis construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.DepositorInsertmRFP1-linker-sfGFP
UseSynthetic BiologyAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-Rab29
Plasmid#186021Purpose2xHis-Rab29DepositorInsert2xHis-Rab29 (RAB29 Human)
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
ExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG021 (AVAST type 5)
Plasmid#157899PurposeExpresses the AVAST type 5 defense systemDepositorInsertAVAST type 5 (STAND ATPase with N-terminal SIR2)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046768
Plasmid#145763PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
ExpressionBacterialMutationPcaU: K14N A165T N201HAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-ZoAl-ENT-3xF-ORF
Plasmid#238484PurposeFor production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINTDepositorInsertR2 retroelement mRNA
UseIvt templateTags3x-FlagMutationR1103AAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-IodoY
Plasmid#113645PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.DepositorInsertIodoYRS and tRNA gene
UseSynthetic BiologyAvailable SinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPM1003
Plasmid#167550PurposeEncodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12DepositorInsertVibrio cholerae O antigens genes of the Ogawa serotype
UseCosmidAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
ExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
EcNusGFL
Plasmid#113122PurposeExpresses full-length E. coli NusGDepositorAvailable SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
ExpressionBacterialAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046959
Plasmid#145722PurposeBacterial Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAJT194
Plasmid#202293PurposeType IIS cloning plasmid for LPattB5 integrations w/ OriTDepositorInsertsfGFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJM.884
Plasmid#108536PurposeAcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.DepositorInsertAcuRAM + PAcu-YFP
ExpressionBacterialMutationAcuR: E80K R154H L202MAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW068
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
ExpressionBacterialPromotercLac143Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM251 (miniCTX1-araC-ParaBAD)
Plasmid#110567PurposeIntegration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
ExpressionBacterialPromoteraraC-ParaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Prha-base
Plasmid#169244PurposeSuicide vector for integrating expression cassette in P. putida (L-rhamnose induction)DepositorInsertrhaRS
ExpressionBacterialPromoterrhaSAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
Tags8xhistidineExpressionYeastPromoterTDH3Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL004
Plasmid#98920PurposeConstitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.DepositorInsertGCaMP6f tethered to mRuby
TagsHis and mRuby3ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkH
Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
TagsmVenusNB internal fusion to MrkHExpressionBacterialPromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsA
Plasmid#107558PurposePlasmid for titrating synthesis of FtsA with doxycyclineDepositorInsertftsA
UseSynthetic BiologyPromoterpLtetOAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJT195
Plasmid#202294PurposeType IIS cloning plasmid for LPattB7 integrations w/ OriTDepositorInsertsfGFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
BB1_23_dCAS9(3xFLAG_SV40NLS)
Plasmid#161478PurposeBB1 encoding a FLAG tagged dCAS9 with a SV40 NLSDepositorInsertdCAS9
UseCRISPR and Synthetic BiologyTagsFLAG Tag, SV40 NLSAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsZ
Plasmid#89656PurposePlasmid for titrating synthesis of FtsZ with doxycyclineDepositorInsertGTP-binding tubulin-like cell division protein
UseSynthetic BiologyAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -